Abstract
Fusarium head blight (FHB), caused by the hemibiotrophic fungus Fusarium graminearum, is one of the major threats to global wheat productivity. A wheat pore-forming toxin-like (PFT) protein was previously reported to underlie Fhb1, the most widely used quantitative trait locus in FHB breeding programs worldwide. In the present work, wheat PFT was ectopically expressed in the model dicot plant Arabidopsis. Heterologous expression of wheat PFT in Arabidopsis provided a broad-spectrum quantitative resistance to fungal pathogens including F. graminearum, Colletotrichum higginsianum, Sclerotinia sclerotiorum, and Botrytis cinerea. However, there was no resistance to bacterial or oomycete pathogens Pseudomonas syringae and Phytophthora capsici, respectively in the transgenic Arabidopsis plants. To explore the reason for the resistance response to, exclusively, the fungal pathogens, purified PFT protein was hybridized to a glycan microarray having 300 different types of carbohydrate monomers and oligomers. It was found that PFT specifically hybridized with chitin monomer, N-acetyl glucosamine (GlcNAc), which is present in fungal cell walls but not in bacteria or oomycete species. This exclusive recognition of chitin may be responsible for the specificity of PFT-mediated resistance to fungal pathogens. Transfer of the atypical quantitative resistance of wheat PFT to a dicot system highlights its potential utility in designing broad-spectrum resistance in diverse host plants. [Formula: see text] Copyright © 2023 The Author(s). This is an open access article distributed under the CC BY-NC-ND 4.0 International license .
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Claims 4
Claim · model-written summary
Heterologous expression of wheat PFT in Arabidopsis provided broad-spectrum quantitative resistance to fungal pathogens.
- Taxon
- Arabidopsis
Arabidopsise1 - Treatment
- Heterologous expression of wheat PFTe1
- Interactor
- F. graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume1pathogen - Second interactor
- Colletotrichum higginsianumColletotrichum higginsianume1pathogen
- Gene
- wheat PFTe1
- Outcome
- broad-spectrum quantitative resistancee1
- Direction
- increaseinferrede1
- Qualifier
- Sclerotinia sclerotiorum, and Botrytis cinereae1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e1“Heterologous expression of wheat PFT in Arabidopsis provided a broad-spectrum quantitative resistance to fungal pathogens including F. graminearum, Colletotrichum higginsianum, Sclerotinia sclerotiorum, and Botrytis cinerea”
Claim · model-written summary
Expression of wheat PFT in Arabidopsis gave no resistance to the bacterial pathogen Pseudomonas syringae or the oomycete Phytophthora capsici.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- Heterologous expression of wheat PFTe1
- Interactor
- Pseudomonas syringae
Pseudomonas syringaee2pathogen - Second interactor
- Phytophthora capsici
.jpg?width=60)
Phytophthora capsicie2pathogen - Outcome
- resistancee2
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e1“Heterologous expression of wheat PFT in Arabidopsis provided a broad-spectrum quantitative resistance to fungal pathogens including F. graminearum, Colletotrichum higginsianum, Sclerotinia sclerotiorum, and Botrytis cinerea”
e2“there was no resistance to bacterial or oomycete pathogens Pseudomonas syringae and Phytophthora capsici, respectively in the transgenic Arabidopsis plants”
Claim · model-written summary
Wheat PFT specifically hybridized with the chitin monomer N-acetyl glucosamine on a glycan microarray.
- Taxon
- wheat
Triticum aestivume4 - Gene
- PFTe3
- Compound
- N-acetyl glucosamine (GlcNAc)e3
- Outcome
- hybridized with chitin monomere3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“PFT specifically hybridized with chitin monomer, N-acetyl glucosamine (GlcNAc), which is present in fungal cell walls but not in bacteria or oomycete species”
e4“A wheat pore-forming toxin-like (PFT) protein was previously reported to underlie Fhb1”
Claim · model-written summary
The specificity of PFT-mediated resistance to fungal pathogens may be due to recognition of chitin.
- Taxon
- wheatTriticum aestivume4
- Gene
- PFTe5
- Compound
- chitine5
- Outcome
- specificity of PFT-mediated resistance to fungal pathogense5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“A wheat pore-forming toxin-like (PFT) protein was previously reported to underlie Fhb1”
e5“This exclusive recognition of chitin may be responsible for the specificity of PFT-mediated resistance to fungal pathogens”
The same questions in other papers
Triticum aestivum · Fusarium graminearum · ArabidopsisResults agree
1 paper in the same direction as this paper
- positive
overexpression · Pseudomonas syringae · ArabidopsisPositive and negative results
1 paper in the same direction as this paper · 3 in the opposite direction
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →pathogen product · Pseudomonas syringae · ArabidopsisResults agree
2 papers in the same direction as this paper
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.