Abstract
Bax inhibitor-1 (BI-1) is an endoplasmic reticulum (ER)-resident cell death suppressor evolutionarily conserved in eukaryotes. The ability of BI-1 to inhibit the biotic and abiotic stresses have been well studied in Arabidopsis, while the functions of wheat BI-1 are largely unknown. In this study, the wheat BI-1 gene TaBI-1.1 was isolated by an RNA-seq analysis of Fusarium graminearum (Fg)-treated wheat. TaBI-1.1 expression was induced by a salicylic acid (SA) treatment and down-regulated by an abscisic acid (ABA) treatment. Based on β-glucuronidase (GUS) staining, TaBI-1.1 was expressed in mature leaves and roots but not in the hypocotyl or young leaves. Constitutive expression of TaBI-1.1 in Arabidopsis enhanced its resistance to Pseudomonas syringae pv. Tomato (Pst) DC3000 infection and induced SA-related gene expression. Additionally, TaBI-1.1 transgenic Arabidopsis exhibited an alleviation of damage caused by high concentrations of SA and decreased the sensitivity to ABA. Consistent with the phenotype, the RNA-seq analysis of 35S::TaBI-1.1 and Col-0 plants showed that TaBI-1.1 was involved in biotic stresses. These results suggested that TaBI-1.1 positively regulates SA signals and plays important roles in the response to biotic stresses. In addition, TaBI-1.1 interacted with the aquaporin TaPIP1, and both them were localized to ER membrane. Furthermore, we demonstrated that TaPIP1 was up-regulated by SA treatment and TaPIP1 transgenic Arabidopsis enhanced the resistance to Pst DC3000 infection. Thus, the interaction between TaBI-1.1 and TaPIP1 on the ER membrane probably occurs in response to SA signals and defense response.
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Claims 15
Claim · model-written summary
TaBI-1.1 was isolated from Fusarium-treated wheat RNA-seq data.
- Taxon
- wheat
Triticum aestivume0 - Interactor
- Fusarium graminearum
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=60)
Fusarium graminearume0pathogen - Gene
- TaBI-1.1e0
- Outcome
- was isolatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_on_Corn_(Zea_mays)_at_Waimea%2C_Hawaii.jpg?width=500)
Verbatim
e0“the wheat BI-1 gene TaBI-1.1 was isolated by an RNA-seq analysis of Fusarium graminearum (Fg)-treated wheat.”
Claim · model-written summary
Salicylic acid induced TaBI-1.1 expression.
- Treatment
- salicylic acid (SA) treatmente0
- Gene
- TaBI-1.1e0
- Outcome
- TaBI-1.1 expressione0
- Direction
- inducede0
Not reportedTaxonComparator
Verbatim
e0“TaBI-1.1 expression was induced by a salicylic acid (SA) treatment”
Claim · model-written summary
Abscisic acid downregulated TaBI-1.1 expression.
Not reportedTaxonComparatorOutcome
Verbatim
e0“down-regulated by an abscisic acid (ABA) treatment.”
Claim · model-written summary
TaBI-1.1 was expressed in mature leaves and roots but absent from hypocotyl and young leaves.
- Gene
- TaBI-1.1e0
- Outcome
- expressed in mature leaves and roots but not in the hypocotyl or young leavese0
Not reportedTaxon
Verbatim
e0“TaBI-1.1 was expressed in mature leaves and roots but not in the hypocotyl or young leaves.”
Claim · model-written summary
Constitutive TaBI-1.1 expression increased Arabidopsis resistance to Pst DC3000.
- Taxon
- Arabidopsis
Arabidopsise0 - Treatment
- Constitutive expression of TaBI-1.1e0
- Interactor
- Pseudomonas syringae pv. Tomato (Pst) DC3000
Pseudomonas syringaee0pathogen - Gene
- TaBI-1.1e0
- Outcome
- resistance to Pseudomonas syringae pv. Tomato (Pst) DC3000 infectione0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Constitutive expression of TaBI-1.1 in Arabidopsis enhanced its resistance to Pseudomonas syringae pv. Tomato (Pst) DC3000 infection”
Claim · model-written summary
Constitutive TaBI-1.1 expression induced SA-related gene expression.
Not reportedTaxonTreatmentComparator
Verbatim
e0“induced SA-related gene expression.”
Claim · model-written summary
TaBI-1.1 transgenic Arabidopsis showed less damage from high SA concentrations.
- Taxon
- ArabidopsisArabidopsise0
- Material
- TaBI-1.1 transgenic Arabidopsise0
- Treatment
- high concentrations of SAe0
- Outcome
- damagee0
- Direction
- alleviatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaBI-1.1 transgenic Arabidopsis exhibited an alleviation of damage caused by high concentrations of SA”
Claim · model-written summary
TaBI-1.1 transgenic Arabidopsis had lower ABA sensitivity.
Not reportedTaxon
Verbatim
e0“decreased the sensitivity to ABA.”
Claim · model-written summary
RNA-seq analysis implicated TaBI-1.1 in biotic stresses.
Not reportedTaxon
Verbatim
e0“the RNA-seq analysis of 35S::TaBI-1.1 and Col-0 plants showed that TaBI-1.1 was involved in biotic stresses.”
Claim · model-written summary
TaBI-1.1 positively regulates SA signals.
Not reportedTaxon
Verbatim
e0“TaBI-1.1 positively regulates SA signals and plays important roles in the response to biotic stresses.”
Claim · model-written summary
TaBI-1.1 interacted with aquaporin TaPIP1.
Not reportedTaxon
Verbatim
e0“TaBI-1.1 interacted with the aquaporin TaPIP1”
Claim · model-written summary
TaBI-1.1 and TaPIP1 localized to the ER membrane.
- Outcome
- localized to ER membranee0
Not reportedTaxon
Verbatim
e0“both them were localized to ER membrane.”
Claim · model-written summary
SA treatment upregulated TaPIP1.
Not reportedTaxonComparator
Verbatim
e0“TaPIP1 was up-regulated by SA treatment”
Claim · model-written summary
TaPIP1 transgenic Arabidopsis had enhanced resistance to Pst DC3000.
- Taxon
- ArabidopsisArabidopsise0
- Material
- TaPIP1 transgenic Arabidopsise0
- Interactor
- Pst DC3000e0pathogen
- Outcome
- resistance to Pst DC3000 infectione0
- Direction
- enhancede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“TaPIP1 transgenic Arabidopsis enhanced the resistance to Pst DC3000 infection.”
Claim · model-written summary
The TaBI-1.1–TaPIP1 interaction on ER membrane probably responds to SA signals and defense response.
Not reportedTaxon
Verbatim
e0“the interaction between TaBI-1.1 and TaPIP1 on the ER membrane probably occurs in response to SA signals and defense response.”
The same questions in other papers
overexpression · Pseudomonas syringae · ArabidopsisPositive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- negative
- no effect
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.