Abstract
We have recently demonstrated that the cellulose breakdown product cellotriose is a damage-associated molecular pattern (DAMP) which induces responses related to the integrity of the cell wall. Activation of downstream responses requires the Arabidopsis malectin domain-containing CELLOOLIGOMER RECEPTOR KINASE1 (CORK1)Citation1. The cellotriose/CORK1 pathway induces immune responses, including NADPH oxidase-mediated reactive oxygen species production, mitogen-activated protein kinase 3/6 phosphorylation-dependent defense gene activation, and the biosynthesis of defense hormones. However, apoplastic accumulation of cell wall breakdown products should also activate cell wall repair mechanisms. We demonstrate that the phosphorylation pattern of numerous proteins involved in the accumulation of an active cellulose synthase complex in the plasma membrane and those for protein trafficking to and within the trans-Golgi network (TGN) are altered within minutes after cellotriose application to Arabidopsis roots. The phosphorylation pattern of enzymes involved in hemicellulose or pectin biosynthesis and the transcript levels for polysaccharide-synthesizing enzymes responded barely to cellotriose treatments. Our data show that the phosphorylation pattern of proteins involved in cellulose biosynthesis and trans-Golgi trafficking is an early target of the cellotriose/CORK1 pathway.
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Claims 4
Claim · model-written summary
Cellotriose application altered within minutes the phosphorylation pattern of numerous proteins for cellulose synthase accumulation and TGN trafficking in Arabidopsis roots.
- Taxon
- Arabidopsis
Arabidopsise1 - Organ / stage
- rootse1
- Treatment
- cellotriose applicatione1
- Outcome
- phosphorylation pattern of numerous proteinse1
- Direction
- alterede1
- Qualifier
- within minutese1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“We demonstrate that the phosphorylation pattern of numerous proteins involved in the accumulation of an active cellulose synthase complex in the plasma membrane and those for protein trafficking to and within the trans-Golgi network (TGN) are altered within minutes after cellotriose application to Arabidopsis roots”
Claim · model-written summary
Phosphorylation pattern of enzymes for hemicellulose or pectin biosynthesis responded barely to cellotriose.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- cellotriose treatmentse1
- Outcome
- phosphorylation pattern of enzymes involved in hemicellulose or pectin biosynthesise1
- Direction
- responded barelye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The phosphorylation pattern of enzymes involved in hemicellulose or pectin biosynthesis and the transcript levels for polysaccharide-synthesizing enzymes responded barely to cellotriose treatments.”
e2“We demonstrate that the phosphorylation pattern of numerous proteins involved in the accumulation of an active cellulose synthase complex in the plasma membrane and those for protein trafficking to and within the trans-Golgi network (TGN) are altered within minutes after cellotriose application to Arabidopsis roots”
Claim · model-written summary
Transcript levels of polysaccharide-synthesizing enzymes responded barely to cellotriose in Arabidopsis.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- cellotriose treatmentse1
- Outcome
- transcript levels for polysaccharide-synthesizing enzymese1
- Direction
- responded barelye1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“The phosphorylation pattern of enzymes involved in hemicellulose or pectin biosynthesis and the transcript levels for polysaccharide-synthesizing enzymes responded barely to cellotriose treatments.”
e2“We demonstrate that the phosphorylation pattern of numerous proteins involved in the accumulation of an active cellulose synthase complex in the plasma membrane and those for protein trafficking to and within the trans-Golgi network (TGN) are altered within minutes after cellotriose application to Arabidopsis roots”
Claim · model-written summary
Phosphorylation of proteins for cellulose biosynthesis and trans-Golgi trafficking is possibly an early target of the cellotriose/CORK1 pathway.
- Taxon
- ArabidopsisArabidopsise2
- Treatment
- cellotriose/CORK1 pathwaye1
- Outcome
- phosphorylation pattern of proteins involved in cellulose biosynthesis and trans-Golgi traffickinge1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“Our data show that the phosphorylation pattern of proteins involved in cellulose biosynthesis and trans-Golgi trafficking is an early target of the cellotriose/CORK1 pathway.”
e2“We demonstrate that the phosphorylation pattern of numerous proteins involved in the accumulation of an active cellulose synthase complex in the plasma membrane and those for protein trafficking to and within the trans-Golgi network (TGN) are altered within minutes after cellotriose application to Arabidopsis roots”