Abstract
Current study explains effective in vitro protocols for somatic embryogenesis and plantlet regeneration in commercially important date palm cvs. Begum Jungi and Ajwa. Spikelet explants of spathes (avg. 17, 28, 32 cm) excised at different intervals were used as initial explants. Results revealed that sterilization of spathes with 50% sodium hypochlorite (NaOCl) solution resulted in significantly highest survival, lowest mortality and contamination in spikelet explants. The spikelet explants obtained from immature spathes (avg. 17 cm) resulted in significantly highest callogenesis in cvs. Begum Jungi (83.6%) and Ajwa (75.6%). Significantly highest callus induced in floral buds in cvs. Begum Jungi (87.3%) and Ajwa (84.3%) on medium comprising of 2,4-D (2.0 mg L -1 ), 2iP (0.5 mg L -1 ). Medium comprising of 0.05 mg L -1 2,4-D, 2.0 mg L -1 2iP, 3 g L -1 activated charcoal induced significantly highest somatic embryos in cvs. Begum Jungi (83.3%) and Ajwa (82.6%). Somatic embryos induced in calli after nine months of initial culture were categorized into repeated and non-repeated. Medium comprising of NAA 0.1 mg L -1 , Kin 1.0 mg L -1 revealed with significantly highest germination of somatic embryos in cvs. Begum Jungi (55.6%) and Ajwa (52.3%). Findings obtained will support to describe proper spathe size and plant growth regulator (PGR) levels for somatic embryogenesis and plantlet regeneration in commercially important cultivars of date palm worldwide.
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Claims 9
Claim · model-written summary
50% sodium hypochlorite gave the highest explant survival and lowest mortality and contamination.
- Taxon
- Date Palm
Phoenix dactyliferat0 - Treatment
- 50% sodium hypochlorite (NaOCl) solutione0
- Outcome
- survival, lowest mortality and contaminatione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“sterilization of spathes with 50% sodium hypochlorite (NaOCl) solution resulted in significantly highest survival, lowest mortality and contamination in spikelet explants”
t0“Date Palm”
Claim · model-written summary
Immature 17 cm spathes produced 83.6% callogenesis in Begum Jungi.
- Taxon
- Date PalmPhoenix dactyliferat0
- Material
- Begum Jungie0
- Treatment
- immature spathes (avg. 17 cm)e0
- Outcome
- callogenesise0
- Direction
- higheste0
- Value
- 83.6%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“immature spathes (avg. 17 cm) resulted in significantly highest callogenesis in cvs. Begum Jungi (83.6%) and Ajwa (75.6%)”
t0“Date Palm”
Claim · model-written summary
Immature 17 cm spathes produced 75.6% callogenesis in Ajwa.
- Taxon
- Date PalmPhoenix dactyliferat0
- Material
- Ajwae0
- Treatment
- immature spathes (avg. 17 cm)e0
- Outcome
- callogenesise0
- Direction
- higheste0
- Value
- 75.6%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“immature spathes (avg. 17 cm) resulted in significantly highest callogenesis in cvs. Begum Jungi (83.6%) and Ajwa (75.6%)”
t0“Date Palm”
Claim · model-written summary
The 2,4-D and 2iP medium gave 87.3% callus induction in Begum Jungi.
- Taxon
- Date PalmPhoenix dactyliferat0
- Material
- Begum Jungie0
- Treatment
- medium comprising of 2,4-D (2.0 mg L -1 ), 2iP (0.5 mg L -1 )e0
- Outcome
- callus inducede0
- Direction
- higheste0
- Value
- 87.3%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significantly highest callus induced in floral buds in cvs. Begum Jungi (87.3%) and Ajwa (84.3%) on medium comprising of 2,4-D (2.0 mg L -1 ), 2iP (0.5 mg L -1 )”
t0“Date Palm”
Claim · model-written summary
The 2,4-D and 2iP medium gave 84.3% callus induction in Ajwa.
- Taxon
- Date PalmPhoenix dactyliferat0
- Material
- Ajwae0
- Treatment
- medium comprising of 2,4-D (2.0 mg L -1 ), 2iP (0.5 mg L -1 )e0
- Outcome
- callus inducede0
- Direction
- higheste0
- Value
- 84.3%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Significantly highest callus induced in floral buds in cvs. Begum Jungi (87.3%) and Ajwa (84.3%) on medium comprising of 2,4-D (2.0 mg L -1 ), 2iP (0.5 mg L -1 )”
t0“Date Palm”
Claim · model-written summary
The activated charcoal medium gave 83.3% somatic embryos in Begum Jungi.
- Taxon
- Date PalmPhoenix dactyliferat0
- Material
- Begum Jungie0
- Treatment
- 0.05 mg L -1 2,4-D, 2.0 mg L -1 2iP, 3 g L -1 activated charcoale0
- Outcome
- somatic embryose0
- Direction
- higheste0
- Value
- 83.3%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Medium comprising of 0.05 mg L -1 2,4-D, 2.0 mg L -1 2iP, 3 g L -1 activated charcoal induced significantly highest somatic embryos in cvs. Begum Jungi (83.3%) and Ajwa (82.6%)”
t0“Date Palm”
Claim · model-written summary
The activated charcoal medium gave 82.6% somatic embryos in Ajwa.
- Taxon
- Date PalmPhoenix dactyliferat0
- Material
- Ajwae0
- Treatment
- 0.05 mg L -1 2,4-D, 2.0 mg L -1 2iP, 3 g L -1 activated charcoale0
- Outcome
- somatic embryose0
- Direction
- higheste0
- Value
- 82.6%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Medium comprising of 0.05 mg L -1 2,4-D, 2.0 mg L -1 2iP, 3 g L -1 activated charcoal induced significantly highest somatic embryos in cvs. Begum Jungi (83.3%) and Ajwa (82.6%)”
t0“Date Palm”
Claim · model-written summary
NAA and Kin medium gave 55.6% embryo germination in Begum Jungi.
- Taxon
- Date PalmPhoenix dactyliferat0
- Material
- Begum Jungie0
- Treatment
- NAA 0.1 mg L -1 , Kin 1.0 mg L -1e0
- Outcome
- germination of somatic embryose0
- Direction
- higheste0
- Value
- 55.6%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Medium comprising of NAA 0.1 mg L -1 , Kin 1.0 mg L -1 revealed with significantly highest germination of somatic embryos in cvs. Begum Jungi (55.6%) and Ajwa (52.3%)”
t0“Date Palm”
Claim · model-written summary
NAA and Kin medium gave 52.3% embryo germination in Ajwa.
- Taxon
- Date PalmPhoenix dactyliferat0
- Material
- Ajwae0
- Treatment
- NAA 0.1 mg L -1 , Kin 1.0 mg L -1e0
- Outcome
- germination of somatic embryose0
- Direction
- higheste0
- Value
- 52.3%e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Medium comprising of NAA 0.1 mg L -1 , Kin 1.0 mg L -1 revealed with significantly highest germination of somatic embryos in cvs. Begum Jungi (55.6%) and Ajwa (52.3%)”
t0“Date Palm”
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