Paper · 2023 · The Journal Agriculture and Forestry

EXTRACTION AND SPECTROPHOTOMETRIC QUANTIFICATION OF TOTAL POLYPHENOLIC CONTENT IN ONION

Mirjana S. JANKULOVSKA; Viktorija SOKOLOVSKA; Lenche VELKOSKA-MARKOVSKA; et al.

3
claims
5
verbatim quotes
1
citations
1
research areas
✓
Open access · cc-by

Abstract

Consumption of food rich in polyphenols such as fruits and vegetables is of great importance, because it contributes to the prevention of various diseases.Polyphenols are a group of bioactive compounds which can play significant role in preventing various health related problems.One of the richest sources of polyphenols in the human diet is the onion (Allium cepa L.).The main objective of the present investigation is quantification of total polyphenolic content (TPC) in the Macedonian local population of onion.A various organic solvents were tested (methanol, ethanol, ethyl acetate and acetone) and their different concentrations (20, 40, 60 and 80%) in order to establish optimum solvent concentration for extraction of polyphenols from onion.Time for extraction was another important parameter which was examined as well.TPC was determined by UV-Vis spectroscopy in accordance with the Folin-Ciocâlteu assay, using gallic acid as a reference standard.The results were expressed as mg gallic acid equivalents GAE/100 g fresh onion.The highest polyphenolic level was determined in 60% methanol (38.81±0.39mg GAE/100 g), while lower total polyphenolic content was found in the ethyl acetate extracts (27.10±0.35mg GAE/100 g).Regarding the time for extraction, the highest content of total polyphenols in onion was obtained during extraction of 120 minutes (37.95±0.63mg GAE/100 g).

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Claims 3

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The highest onion polyphenolic level was obtained with 60% methanol.

Taxon
onionAllium cepat0
Treatment
60% methanole0
Outcome
polyphenolic levele0
Direction
higheste0
Value
38.81±0.39mg GAE/100 ge0
Comparator
ethyl acetate extractse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest polyphenolic level was determined in 60% methanol (38.81±0.39mg GAE/100 g), while lower total polyphenolic content was found in the ethyl acetate extracts (27.10±0.35mg GAE/100 g).”

✓ located verbatim in the source · abstract, characters 1039–1231 · show in text ↑
t0

“ONION”

✓ located verbatim in the source · title, characters 82–87 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Ethyl acetate extracts had lower onion total polyphenolic content than 60% methanol.

Taxon
onionAllium cepat0
Treatment
ethyl acetate extractse0
Outcome
total polyphenolic contente0
Direction
lowere0
Value
27.10±0.35mg GAE/100 ge0
Comparator
60% methanole0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“The highest polyphenolic level was determined in 60% methanol (38.81±0.39mg GAE/100 g), while lower total polyphenolic content was found in the ethyl acetate extracts (27.10±0.35mg GAE/100 g).”

✓ located verbatim in the source · abstract, characters 1039–1231 · show in text ↑
t0

“ONION”

✓ located verbatim in the source · title, characters 82–87 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Extraction for 120 minutes yielded the highest total polyphenol content in onion.

Taxon
onionAllium cepae0
Treatment
extraction of 120 minutese0
Outcome
content of total polyphenolse0
Direction
higheste0
Value
37.95±0.63mg GAE/100 ge0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Allium cepa
Allium cepa · TaxonMehdi · CC BY-SA 3.0 · Wikimedia Commons ↗

Verbatim

e0

“Regarding the time for extraction, the highest content of total polyphenols in onion was obtained during extraction of 120 minutes (37.95±0.63mg GAE/100 g).”

✓ located verbatim in the source · abstract, characters 1231–1387 · show in text ↑

botanical · molecular: metabolites · Allium cepa (onion)Positive and negative results

13 papers: 6 positive, 7 negative or no effect

6 papers in the same direction as this paper · 6 in the opposite direction

  1. positive
  2. positive
  3. positive

9 papers more in this group

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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.