Abstract
Zinc (Zn) is an essential micronutrient for plants. Adequate regulation of Zn uptake, transport and distribution, and adaptation to Zn-deficiency stress or Zn-excess toxicity are crucial for plant growth and development. However, little has been done to understand the molecular responses of plants toward different Zn supply levels. In the present study, we investigated the growth and physiological responses of tobacco seedlings grown under Zn-completely deficient, Zn-limiting, Zn-normal, and Zn-4-fold sufficient conditions, respectively, and demonstrated that Zn deficiency/limitation caused oxidative stress and impaired growth of tobacco plants. Combined transcriptome and proteome analysis revealed up-regulation of genes/proteins associated with Zn uptake and distribution, including ZIPs, NAS3s, and HMA1s, and up-regulation of genes/proteins involved in regulation of oxidative stress, including SODs, APX1s, GPX6, and GSTs in tobacco seedlings in response to Zn deficiency/limitation, suggesting that tobacco possessed mechanisms to regulate Zn homeostasis primarily through up-regulation of the ZIPs-NAS3s module, and to alleviate Zn deficiency/limitation-induced oxidative stress through activation of the antioxidant machinery. Our results provide novel insights into the adaptive mechanisms of tobacco in response to different Zn supplies, and would lay a theoretical foundation for development of varieties of tobacco or its relatives with high tolerance to Zn-deficiency.
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Claims 6
Claim · model-written summary
Zinc deficiency or limitation caused oxidative stress in tobacco plants.
- Taxon
- tobacco plants
Nicotiana tabacume2 - Treatment
- Zn deficiency/limitatione2
- Outcome
- oxidative stresse2
- Direction
- causede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Zn deficiency/limitation caused oxidative stress and impaired growth of tobacco plants”
Claim · model-written summary
Zinc deficiency or limitation impaired growth of tobacco plants.
- Taxon
- tobacco plantsNicotiana tabacume2
- Treatment
- Zn deficiency/limitatione2
- Outcome
- growthe2
- Direction
- impairede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e2“Zn deficiency/limitation caused oxidative stress and impaired growth of tobacco plants”
Claim · model-written summary
Zinc deficiency or limitation up-regulated genes and proteins for zinc uptake and distribution (ZIPs, NAS3s, HMA1s) in tobacco seedlings.
- Taxon
- tobacco seedlingsNicotiana tabacume3
- Treatment
- Zn deficiency/limitatione3
- Gene
- ZIPs, NAS3s, and HMA1se3
- Outcome
- genes/proteins associated with Zn uptake and distributione3
- Direction
- up-regulatione3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Combined transcriptome and proteome analysis revealed up-regulation of genes/proteins associated with Zn uptake and distribution, including ZIPs, NAS3s, and HMA1s, and up-regulation of genes/proteins involved in regulation of oxidative stress, including SODs, APX1s, GPX6, and GSTs in tobacco seedlings in response to Zn deficiency/limitation”
Claim · model-written summary
Zinc deficiency or limitation up-regulated genes and proteins for oxidative stress regulation (SODs, APX1s, GPX6, GSTs) in tobacco seedlings.
- Taxon
- tobacco seedlingsNicotiana tabacume3
- Treatment
- Zn deficiency/limitatione3
- Gene
- SODs, APX1s, GPX6, and GSTse3
- Outcome
- genes/proteins involved in regulation of oxidative stresse3
- Direction
- up-regulatione3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Combined transcriptome and proteome analysis revealed up-regulation of genes/proteins associated with Zn uptake and distribution, including ZIPs, NAS3s, and HMA1s, and up-regulation of genes/proteins involved in regulation of oxidative stress, including SODs, APX1s, GPX6, and GSTs in tobacco seedlings in response to Zn deficiency/limitation”
Claim · model-written summary
Tobacco appears to regulate zinc homeostasis primarily through up-regulation of the ZIPs-NAS3s module.
- Taxon
- tobaccoNicotiana tabacume4
- Gene
- ZIPs-NAS3se4
- Outcome
- regulate Zn homeostasise4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“suggesting that tobacco possessed mechanisms to regulate Zn homeostasis primarily through up-regulation of the ZIPs-NAS3s module, and to alleviate Zn deficiency/limitation-induced oxidative stress through activation of the antioxidant machinery”
Claim · model-written summary
Tobacco appears to alleviate zinc deficiency-induced oxidative stress through activation of the antioxidant machinery.
- Taxon
- tobaccoNicotiana tabacume4
- Outcome
- Zn deficiency/limitation-induced oxidative stresse4
- Qualifier
- through activation of the antioxidant machinerye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“suggesting that tobacco possessed mechanisms to regulate Zn homeostasis primarily through up-regulation of the ZIPs-NAS3s module, and to alleviate Zn deficiency/limitation-induced oxidative stress through activation of the antioxidant machinery”
The same questions in other papers
zinc atom · physiology: oxidative · Nicotiana tabacum (tobacco)Positive and negative results
1 paper in the same direction as this paper · 2 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- negative
- no effect
- positive
zinc atom · growth · Nicotiana tabacum (tobacco)Positive and negative results
8 papers in the same direction as this paper · 1 in the opposite direction
This paper is about lacking the nutrient, so it counts the other way round here.
- negative
- positive
- positive
6 papers more in this group
See all 10 papers →zinc atom · molecular: expression · Nicotiana tabacum (tobacco)Results agree
3 papers in the same direction as this paper
This paper is about lacking the nutrient, so it counts the other way round here.
- no effect
- no effect
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.