Abstract
Hippocratea africana, a medicinal plant, used in ethnomedicine for the treatment of antimalarial, diabetes, poisoning and inflammatory diseases was investigated for cytotoxic and genotoxic effects on the root meristem cells of Allium cepa. The extract and fractions was also screened for total flavonoid and phenol content using standard methods. Onion bulbs were exposed to 2.5 mg/ml, 5mg/ml, and 10 mg/ml concentrations of the leaf extract for macroscopic and microscopic analysis. Tap water was used as a negative control and Methotrexate (0.1 mg/ml) as a positive control. There was statistically significant (P < 0.05) inhibition of root growth depending on concentration by the extract when compared with the negative control group. All the tested concentrations of the extract were observed to have cytotoxic effects on cell division in A. cepa. The extract- induced chromosomal aberrations and micronuclei (MNC) formations in A. cepa root tip cells and were significant (p<0.05) when compared with control group. The extract treatment further induced cell death, ghost cells, cells membrane damage, and binucleated cells. These results suggest that Hippocratea africana root extract possess cytotoxic and genotoxic effects on A. cepa which is as a results of its phytochemical constituents.
Abstract from the paper’s OpenAlex record, shown for indexing and research. Copyright remains with the authors or the publisher. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 5
Claim · model-written summary
Extract inhibited onion root growth with concentration dependence.
- Taxon
- Allium cepa
Allium cepae1 - Treatment
- extracte0
- Outcome
- root growthe0
- Direction
- inhibitione0
- Qualifier
- depending on concentratione0
- Comparator
- negative control groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“There was statistically significant (P < 0.05) inhibition of root growth depending on concentration by the extract when compared with the negative control group.”
e1“root meristem cells of Allium cepa”
Claim · model-written summary
Every tested extract concentration had cytotoxic effects on onion cell division.
- Taxon
- A. cepaAllium cepae0
- Treatment
- extracte0
- Outcome
- cell divisione0
- Direction
- cytotoxic effectse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“All the tested concentrations of the extract were observed to have cytotoxic effects on cell division in A. cepa.”
Claim · model-written summary
Extract induced significant chromosomal aberrations and micronuclei in onion root tips.
- Taxon
- A. cepaAllium cepae0
- Organ / stage
- root tip cellse0
- Treatment
- extracte0
- Outcome
- chromosomal aberrations and micronuclei (MNC) formationse0
- Direction
- inducede0
- Comparator
- control groupe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The extract- induced chromosomal aberrations and micronuclei (MNC) formations in A. cepa root tip cells and were significant (p<0.05) when compared with control group.”
Claim · model-written summary
Extract induced cell death, ghost cells and membrane damage.
- Taxon
- Allium cepaAllium cepae1
- Treatment
- extract treatmente0
- Outcome
- cell death, ghost cells, cells membrane damagee0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The extract treatment further induced cell death, ghost cells, cells membrane damage, and binucleated cells.”
e1“root meristem cells of Allium cepa”
Claim · model-written summary
Extract induced binucleated cells.
- Taxon
- Allium cepaAllium cepae1
- Treatment
- extract treatmente0
- Outcome
- binucleated cellse0
- Direction
- inducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The extract treatment further induced cell death, ghost cells, cells membrane damage, and binucleated cells.”
e1“root meristem cells of Allium cepa”
The same questions in other papers
botanical · physiology: oxidative · Allium cepa (onion)Positive and negative results
5 papers in the same direction as this paper · 10 in the opposite direction
- positive
- positive
- positive
12 papers more in this group
See all 16 papers →botanical · growth · Allium cepa (onion)Positive and negative results
51 papers in the same direction as this paper · 37 in the opposite direction
- positive
- positive
- positive
85 papers more in this group
See all 89 papers →botanical · cytology · Allium cepa (onion)Positive and negative results
89 papers in the same direction as this paper · 26 in the opposite direction
- positive
- positive
- positive
112 papers more in this group
See all 116 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.