Abstract
Sugarcane is an economically important crop plant across the globe as it is the primary source of sugar and biofuel. Its growth and development are greatly influenced by water availability; therefore, in periods of water scarcity, yields are severely compromised. Small Ubiquitin-Like Modifier (SUMO) proteases play an important role in stress responses by regulating the SUMO-related post-translational modification of proteins. In an attempt to enhance drought tolerance in sugarcane, this crop was genetically transformed with a cysteine protease (OVERLY TOLERANT TO SALT-1; OTS1) from Arabidopsis thaliana using particle bombardment. Transgenic plants were analysed in terms of photosynthetic capacity, oxidative damage, antioxidant accumulation and the SUMO-enrich protein profile was assessed. Sugarcane transformed with the AtOTS1 gene displayed enhanced drought tolerance and delayed leaf senescence under water deficit compared to the untransformed wild type (WT). The AtOTS1 transgenic plants maintained a high relative moisture content and higher photosynthesis rate when compared to the WT. In addition, when the transgene was expressed at high levels, the transformed plants were able to maintain higher stomatal conductance and chlorophyl content under moderate stress compared to the WT. Under severe water deficit stress, the transgenic plants accumulated less malondialdehyde and maintained membrane integrity. SUMOylation of total protein and protease activity was lower in the AtOTS1 transformed plants compared to the WT, with several SUMO-enriched proteins exclusively expressed in the transgenics when exposed to water deficit stress. SUMOylation of proteins likely influenced various mechanisms contributing to enhanced drought tolerance in sugarcane.
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Claims 11
Claim · model-written summary
AtOTS1 transformation enhanced drought tolerance of sugarcane under water deficit compared with the wild type.
Verbatim
t0“sugarcane (Saccharum spp. hybrid)”
e1“Sugarcane transformed with the AtOTS1 gene displayed enhanced drought tolerance and delayed leaf senescence under water deficit compared to the untransformed wild type (WT)”
Claim · model-written summary
AtOTS1 transformation delayed leaf senescence of sugarcane under water deficit compared with the wild type.
Verbatim
t0“sugarcane (Saccharum spp. hybrid)”
e1“Sugarcane transformed with the AtOTS1 gene displayed enhanced drought tolerance and delayed leaf senescence under water deficit compared to the untransformed wild type (WT)”
Claim · model-written summary
AtOTS1 transgenic sugarcane maintained a high relative moisture content compared with the wild type.
Verbatim
t0“sugarcane (Saccharum spp. hybrid)”
e1“Sugarcane transformed with the AtOTS1 gene displayed enhanced drought tolerance and delayed leaf senescence under water deficit compared to the untransformed wild type (WT)”
e2“The AtOTS1 transgenic plants maintained a high relative moisture content and higher photosynthesis rate when compared to the WT”
Claim · model-written summary
AtOTS1 transgenic sugarcane had a higher photosynthesis rate than the wild type.
Verbatim
t0“sugarcane (Saccharum spp. hybrid)”
e1“Sugarcane transformed with the AtOTS1 gene displayed enhanced drought tolerance and delayed leaf senescence under water deficit compared to the untransformed wild type (WT)”
e2“The AtOTS1 transgenic plants maintained a high relative moisture content and higher photosynthesis rate when compared to the WT”
Claim · model-written summary
At high transgene expression, AtOTS1 sugarcane had higher stomatal conductance than the wild type under moderate stress.
Verbatim
t0“sugarcane (Saccharum spp. hybrid)”
e3“when the transgene was expressed at high levels, the transformed plants were able to maintain higher stomatal conductance and chlorophyl content under moderate stress compared to the WT”
Claim · model-written summary
At high transgene expression, AtOTS1 sugarcane had higher chlorophyll content than the wild type under moderate stress.
Verbatim
t0“sugarcane (Saccharum spp. hybrid)”
e3“when the transgene was expressed at high levels, the transformed plants were able to maintain higher stomatal conductance and chlorophyl content under moderate stress compared to the WT”
Claim · model-written summary
Under severe water deficit, AtOTS1 sugarcane accumulated less malondialdehyde.
Not reportedComparator
Verbatim
t0“sugarcane (Saccharum spp. hybrid)”
e4“Under severe water deficit stress, the transgenic plants accumulated less malondialdehyde and maintained membrane integrity”
Claim · model-written summary
Under severe water deficit, AtOTS1 sugarcane maintained membrane integrity.
- Taxon
- sugarcanet0
- Treatment
- severe water deficit stresse4
- Outcome
- membrane integritye4
- Direction
- maintainede4
Not reportedComparator
Verbatim
t0“sugarcane (Saccharum spp. hybrid)”
e4“Under severe water deficit stress, the transgenic plants accumulated less malondialdehyde and maintained membrane integrity”
Claim · model-written summary
SUMOylation of total protein and protease activity were lower in AtOTS1 transformed sugarcane than in the wild type.
Verbatim
t0“sugarcane (Saccharum spp. hybrid)”
e5“SUMOylation of total protein and protease activity was lower in the AtOTS1 transformed plants compared to the WT”
Claim · model-written summary
Several SUMO-enriched proteins were expressed exclusively in the AtOTS1 transgenics under water deficit stress.
Verbatim
t0“sugarcane (Saccharum spp. hybrid)”
e6“with several SUMO-enriched proteins exclusively expressed in the transgenics when exposed to water deficit stress”
Claim · model-written summary
SUMOylation of proteins likely influenced mechanisms contributing to enhanced drought tolerance in sugarcane.
Verbatim
t0“sugarcane (Saccharum spp. hybrid)”
e7“SUMOylation of proteins likely influenced various mechanisms contributing to enhanced drought tolerance in sugarcane”
The same questions in other papers
drought · physiology: oxidative · Saccharum officinarum (sugarcane)Positive and negative results
2 papers in the same direction as this paper · 7 in the opposite direction
- negative
- no effect
- negative
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.