Abstract
LncRNAs have gained increasing attention owing to their important regulatory roles on growth and stress responses of plants. However, the mechanisms underlying the functions of lncRNAs in fruit-pathogen interaction are still largely unknown. In this study, a total of 273 lncRNAs responding to Botrytis cinerea infection were identified in tomato fruit, among which a higher percentage of antisense lncRNAs were targeted to the genes enriched in hydrolase activity. To ascertain the roles of these lncRNAs, seven hydrolase-related transcripts were transiently knocked-down by virus-induced gene silencing. Silencing of lncRNACXE20 reduced the expression level of a carboxylesterase gene, further enhancing the resistance of tomato to B. cinerea. In contrast, silencing of lncRNACHI, lncRNAMMP, lncRNASBT1.9 and lncRNAPME1.9 impaired the resistance to B. cinerea, respectively. Further RT-qPCR assay and enzymatic activity detection displayed that the attenuated resistance of lncRNAMMP and lncRNASBT1.9-silenced plants was associated with the inhibition on the expression of JA-related genes, while the decreased resistance of lncRNACHI-silenced plants resulted in reduced chitinase activity. Collectively, these results may provide references for deciphering the mechanisms underlying specific lncRNAs to interfere with B. cinerea infection by regulating the expression of defence-related genes or affecting hydrolase activity.
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Claims 11
Claim · model-written summary
The study identified 273 lncRNAs responding to Botrytis cinerea infection in tomato fruit.
- Taxon
- tomato
Solanum lycopersicume0 - Organ / stage
- fruite0
- Interactor
- Botrytis cinerea
Botrytis cinereae0pathogen - Outcome
- lncRNAs responding to Botrytis cinerea infectione0
- Value
- 273e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a total of 273 lncRNAs responding to Botrytis cinerea infection were identified in tomato fruit”
Claim · model-written summary
A higher percentage of antisense lncRNAs targeted genes enriched in hydrolase activity.
- Taxon
- tomatoSolanum lycopersicumt0
- Outcome
- antisense lncRNAs were targeted to the genes enriched in hydrolase activitye0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a higher percentage of antisense lncRNAs were targeted to the genes enriched in hydrolase activity”
t0“tomato”
Claim · model-written summary
Silencing lncRNACXE20 reduced carboxylesterase gene expression.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- Silencing of lncRNACXE20e0
- Gene
- lncRNACXE20e0
- Outcome
- expression level of a carboxylesterase genee0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of lncRNACXE20 reduced the expression level of a carboxylesterase gene”
t0“tomato”
Claim · model-written summary
Silencing lncRNACXE20 enhanced tomato resistance to B. cinerea.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- Silencing of lncRNACXE20e0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- lncRNACXE20e0
- Outcome
- resistance of tomato to B. cinereae0
- Direction
- enhancinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Silencing of lncRNACXE20 reduced the expression level of a carboxylesterase gene, further enhancing the resistance of tomato to B. cinerea”
Claim · model-written summary
Silencing lncRNACHI impaired tomato resistance to B. cinerea.
- Taxon
- tomatoSolanum lycopersicumt0
- Treatment
- silencing of lncRNACHIe0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- lncRNACHIe0
- Outcome
- resistance to B. cinereae0
- Direction
- impairede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of lncRNACHI, lncRNAMMP, lncRNASBT1.9 and lncRNAPME1.9 impaired the resistance to B. cinerea”
t0“tomato”
Claim · model-written summary
Silencing lncRNAMMP impaired tomato resistance to B. cinerea.
- Taxon
- tomatoSolanum lycopersicumt0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- lncRNAMMPe0
- Outcome
- resistance to B. cinereae0
- Direction
- impairede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lncRNAMMP, lncRNASBT1.9 and lncRNAPME1.9 impaired the resistance to B. cinerea”
t0“tomato”
Claim · model-written summary
Silencing lncRNASBT1.9 impaired tomato resistance to B. cinerea.
- Taxon
- tomatoSolanum lycopersicumt0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- lncRNASBT1.9e0
- Outcome
- resistance to B. cinereae0
- Direction
- impairede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lncRNASBT1.9 and lncRNAPME1.9 impaired the resistance to B. cinerea”
t0“tomato”
Claim · model-written summary
Silencing lncRNAPME1.9 impaired tomato resistance to B. cinerea.
- Taxon
- tomatoSolanum lycopersicumt0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- lncRNAPME1.9e0
- Outcome
- resistance to B. cinereae0
- Direction
- impairede0
Not reportedTreatmentComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“lncRNAPME1.9 impaired the resistance to B. cinerea”
t0“tomato”
Claim · model-written summary
Reduced resistance in lncRNAMMP-silenced plants was associated with inhibited JA-related gene expression.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- lncRNAMMPe0
- Outcome
- attenuated resistancee0
- Direction
- attenuatede0
- Qualifier
- inhibition on the expression of JA-related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the attenuated resistance of lncRNAMMP and lncRNASBT1.9-silenced plants was associated with the inhibition on the expression of JA-related genes”
t0“tomato”
Claim · model-written summary
Reduced resistance in lncRNASBT1.9-silenced plants was associated with inhibited JA-related gene expression.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- lncRNASBT1.9e0
- Outcome
- attenuated resistancee0
- Direction
- attenuatede0
- Qualifier
- inhibition on the expression of JA-related genese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the attenuated resistance of lncRNAMMP and lncRNASBT1.9-silenced plants was associated with the inhibition on the expression of JA-related genes”
t0“tomato”
Claim · model-written summary
Reduced resistance in lncRNACHI-silenced plants resulted in reduced chitinase activity.
- Taxon
- tomatoSolanum lycopersicumt0
- Gene
- lncRNACHIe0
- Outcome
- chitinase activitye0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the decreased resistance of lncRNACHI-silenced plants resulted in reduced chitinase activity”
t0“tomato”
The same questions in other papers
loss of function · Botrytis cinerea · Solanum lycopersicum (tomato)Positive and negative results
7 papers in the same direction as this paper · 18 in the opposite direction
- negative
- negative
- negative
22 papers more in this group
See all 26 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.