Abstract
The gaseous signaling molecule nitric oxide (NO) plays an important role in breaking seed dormancy. NO induces a decrease in abscisic acid (ABA) content by transcriptionally activating its catabolic enzyme, the ABA 8'-hydroxylase CYP707A2. However, the underlying mechanism of this process remains unclear. Here, we report that the transcription factor MYB30 plays a critical role in NO-induced seed germination in Arabidopsis (Arabidopsis thaliana). MYB30 loss-of-function attenuates NO-mediated seed dormancy breaking. MYB30 triggers a NO-induced decrease in ABA content during germination by directly promoting CYP707A2 expression. NO induces S-nitrosylation at Cys-49 of MYB30 and enhances its transcriptional activity. Conversely, the ABA receptors PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR) interact with MYB30 and repress its transcriptional activity. ABA promotes the interaction between PYL4 and MYB30, whereas S-nitrosylation releases the PYL4-mediated inhibition of MYB30 by interfering with the PYL4-MYB30 interaction. Genetic analysis showed that MYB30 functions downstream of PYLs during seed dormancy and germination in response to NO. Furthermore, MYB30 mutation significantly represses the reduced dormancy phenotype and the enhanced CYP707A2 expression of the pyr1 pyl1 pyl2 pyl4 quadruple mutant. Our findings reveal that S-nitrosylation of MYB30 precisely regulates the balance of seed dormancy and germination, providing insights into the underlying mechanism of NO-promoted seed germination.
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Claims 13
Claim · model-written summary
MYB30 plays a critical role in NO-induced Arabidopsis seed germination.
- Taxon
- Arabidopsis
Arabidopsise0 - Gene
- MYB30e0
- Outcome
- NO-induced seed germinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“transcription factor MYB30 plays a critical role in NO-induced seed germination in Arabidopsis (Arabidopsis thaliana)”
Claim · model-written summary
MYB30 loss of function attenuates NO-mediated dormancy breaking.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- MYB30 loss-of-functione0
- Gene
- MYB30e0
- Outcome
- NO-mediated seed dormancy breakinge0
- Direction
- attenuatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYB30 loss-of-function attenuates NO-mediated seed dormancy breaking”
t0“Arabidopsis”
Claim · model-written summary
MYB30 promotes CYP707A2 expression directly.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- CYP707A2e0
- Outcome
- CYP707A2 expressione0
- Direction
- promotinge0
- Qualifier
- MYB30 triggerse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYB30 triggers a NO-induced decrease in ABA content during germination by directly promoting CYP707A2 expression”
t0“Arabidopsis”
Claim · model-written summary
MYB30 triggers a NO-induced decrease in ABA during germination.
- Taxon
- ArabidopsisArabidopsist0
- Organ / stage
- germinatione0
- Gene
- MYB30e0
- Outcome
- ABA contente0
- Direction
- decreasee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYB30 triggers a NO-induced decrease in ABA content during germination by directly promoting CYP707A2 expression”
t0“Arabidopsis”
Claim · model-written summary
NO induces MYB30 S-nitrosylation at Cys-49.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- NOe0
- Gene
- MYB30e0
- Outcome
- S-nitrosylation at Cys-49e0
- Direction
- inducese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO induces S-nitrosylation at Cys-49 of MYB30 and enhances its transcriptional activity”
t0“Arabidopsis”
Claim · model-written summary
NO enhances MYB30 transcriptional activity.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- NOe0
- Gene
- MYB30e0
- Outcome
- transcriptional activitye0
- Direction
- enhancese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“NO induces S-nitrosylation at Cys-49 of MYB30 and enhances its transcriptional activity”
t0“Arabidopsis”
Claim · model-written summary
ABA receptors interact with MYB30.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- MYB30e0
- Outcome
- interact with MYB30e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA receptors PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR) interact with MYB30”
t0“Arabidopsis”
Claim · model-written summary
ABA receptors repress MYB30 transcriptional activity.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- MYB30e0
- Outcome
- transcriptional activitye0
- Direction
- represse0
- Qualifier
- ABA receptorse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA receptors PYRABACTIN RESISTANCE1 (PYR1)/PYR1-LIKE (PYL)/REGULATORY COMPONENTS OF ABA RECEPTORS (RCAR) interact with MYB30 and repress its transcriptional activity”
t0“Arabidopsis”
Claim · model-written summary
ABA promotes interaction between PYL4 and MYB30.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- ABAe0
- Gene
- PYL4 and MYB30e0
- Outcome
- interaction between PYL4 and MYB30e0
- Direction
- promotese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ABA promotes the interaction between PYL4 and MYB30”
t0“Arabidopsis”
Claim · model-written summary
S-nitrosylation releases PYL4 inhibition of MYB30 by interfering with their interaction.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- MYB30e0
- Outcome
- PYL4-mediated inhibition of MYB30e0
- Direction
- releasese0
- Qualifier
- interfering with the PYL4-MYB30 interactione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“S-nitrosylation releases the PYL4-mediated inhibition of MYB30 by interfering with the PYL4-MYB30 interaction”
t0“Arabidopsis”
Claim · model-written summary
MYB30 functions downstream of PYLs in NO-responsive dormancy and germination.
- Taxon
- ArabidopsisArabidopsist0
- Gene
- MYB30e0
- Outcome
- seed dormancy and germinatione0
- Qualifier
- downstream of PYLse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYB30 functions downstream of PYLs during seed dormancy and germination in response to NO”
t0“Arabidopsis”
Claim · model-written summary
MYB30 mutation significantly represses reduced dormancy of the pyr1 pyl1 pyl2 pyl4 mutant.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- MYB30 mutatione0
- Outcome
- reduced dormancy phenotypee0
- Direction
- repressese0
- Comparator
- pyr1 pyl1 pyl2 pyl4 quadruple mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYB30 mutation significantly represses the reduced dormancy phenotype and the enhanced CYP707A2 expression of the pyr1 pyl1 pyl2 pyl4 quadruple mutant”
t0“Arabidopsis”
Claim · model-written summary
MYB30 mutation significantly represses enhanced CYP707A2 expression of the pyr1 pyl1 pyl2 pyl4 mutant.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- MYB30 mutatione0
- Gene
- CYP707A2e0
- Outcome
- enhanced CYP707A2 expressione0
- Direction
- repressese0
- Comparator
- pyr1 pyl1 pyl2 pyl4 quadruple mutante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“MYB30 mutation significantly represses the reduced dormancy phenotype and the enhanced CYP707A2 expression of the pyr1 pyl1 pyl2 pyl4 quadruple mutant”
t0“Arabidopsis”
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See all 234 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.