Abstract
A reliable and stable hydrogen gas (H2) supply will benefit agricultural laboratory and field trials. Here, we assessed ammonia borane (AB), an efficient hydrogen storage material used in the energy industry, and determined its effect on plant physiology and the corresponding mechanism. Through hydroponics and pot experiments, we discovered that AB increases tomato (Solanum lycopersicum) lateral root (LR) branching and this function depended on the increased endogenous H2 level caused by the sustainable H2 supply. In particular, AB might trigger LR primordia initiation. Transgenic tomato and Arabidopsis (Arabidopsis thaliana) expressing hydrogenase1 (CrHYD1) from Chlamydomonas reinhardtii not only accumulated higher endogenous H2 and phytomelatonin levels but also displayed pronounced LR branching. These endogenous H2 responses achieved by AB or genetic manipulation were sensitive to the pharmacological removal of phytomelatonin, indicating the downstream role of phytomelatonin in endogenous H2 control of LR formation. Consistently, extra H2 supply failed to influence the LR defective phenotypes in phytomelatonin synthetic mutants. Molecular evidence showed that the phytomelatonin-regulated auxin signaling network and cell-cycle regulation were associated with the AB/H2 control of LR branching. Also, AB and melatonin had little effect on LR branching in the presence of auxin synthetic inhibitors. Collectively, our integrated approaches show that supplying H2 via AB increases LR branching via phytomelatonin signaling. This finding might open the way for applying hydrogen storage materials to horticultural production.
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Claims 10
Claim · model-written summary
Ammonia borane (AB) increased lateral root branching in tomato in hydroponic and pot experiments.
- Taxon
- tomato (Solanum lycopersicum)
Solanum lycopersicume0 - Treatment
- ABe0
- Outcome
- lateral root (LR) branchinge0
- Direction
- increasese0
- Setting
- hydroponics and pot experimentse1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we discovered that AB increases tomato (Solanum lycopersicum) lateral root (LR) branching”
e1“Through hydroponics and pot experiments”
Claim · model-written summary
AB might trigger lateral root primordia initiation in tomato.
- Taxon
- tomato (Solanum lycopersicum)Solanum lycopersicume0
- Treatment
- ABe2
- Outcome
- LR primordia initiatione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we discovered that AB increases tomato (Solanum lycopersicum) lateral root (LR) branching”
e2“In particular, AB might trigger LR primordia initiation”
Claim · model-written summary
Transgenic tomato expressing CrHYD1 accumulated higher endogenous H2 and phytomelatonin levels.
- Taxon
- tomatoSolanum lycopersicume3
- Treatment
- expressing hydrogenase1 (CrHYD1) from Chlamydomonas reinhardtiie3
- Gene
- hydrogenase1 (CrHYD1)e3
- Outcome
- endogenous H2 and phytomelatonin levelse3
- Direction
- highere3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Transgenic tomato and Arabidopsis (Arabidopsis thaliana) expressing hydrogenase1 (CrHYD1) from Chlamydomonas reinhardtii not only accumulated higher endogenous H2 and phytomelatonin levels but also displayed pronounced LR branching”
Claim · model-written summary
Transgenic tomato expressing CrHYD1 displayed pronounced lateral root branching.
- Taxon
- tomatoSolanum lycopersicume3
- Treatment
- expressing hydrogenase1 (CrHYD1) from Chlamydomonas reinhardtiie3
- Gene
- hydrogenase1 (CrHYD1)e3
- Outcome
- LR branchinge3
- Direction
- pronouncede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Transgenic tomato and Arabidopsis (Arabidopsis thaliana) expressing hydrogenase1 (CrHYD1) from Chlamydomonas reinhardtii not only accumulated higher endogenous H2 and phytomelatonin levels but also displayed pronounced LR branching”
Claim · model-written summary
Transgenic Arabidopsis expressing CrHYD1 accumulated higher endogenous H2 and phytomelatonin levels.
- Taxon
- Arabidopsis (Arabidopsis thaliana)
Arabidopsis thalianae3 - Treatment
- expressing hydrogenase1 (CrHYD1) from Chlamydomonas reinhardtiie3
- Gene
- hydrogenase1 (CrHYD1)e3
- Outcome
- endogenous H2 and phytomelatonin levelse3
- Direction
- highere3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Transgenic tomato and Arabidopsis (Arabidopsis thaliana) expressing hydrogenase1 (CrHYD1) from Chlamydomonas reinhardtii not only accumulated higher endogenous H2 and phytomelatonin levels but also displayed pronounced LR branching”
Claim · model-written summary
Transgenic Arabidopsis expressing CrHYD1 displayed pronounced lateral root branching.
- Taxon
- Arabidopsis (Arabidopsis thaliana)Arabidopsis thalianae3
- Treatment
- expressing hydrogenase1 (CrHYD1) from Chlamydomonas reinhardtiie3
- Gene
- hydrogenase1 (CrHYD1)e3
- Outcome
- LR branchinge3
- Direction
- pronouncede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e3“Transgenic tomato and Arabidopsis (Arabidopsis thaliana) expressing hydrogenase1 (CrHYD1) from Chlamydomonas reinhardtii not only accumulated higher endogenous H2 and phytomelatonin levels but also displayed pronounced LR branching”
Claim · model-written summary
Endogenous H2 responses to AB or genetic manipulation are sensitive to removal of phytomelatonin, indicating phytomelatonin acts downstream in H2 control of lateral root formation.
- Taxon
- tomato (Solanum lycopersicum)Solanum lycopersicume0
- Treatment
- pharmacological removal of phytomelatonine4
- Compound
- phytomelatonine4
- Outcome
- endogenous H2 responsese4
- Direction
- sensitivee4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we discovered that AB increases tomato (Solanum lycopersicum) lateral root (LR) branching”
e4“These endogenous H2 responses achieved by AB or genetic manipulation were sensitive to the pharmacological removal of phytomelatonin, indicating the downstream role of phytomelatonin in endogenous H2 control of LR formation”
Claim · model-written summary
Extra H2 supply failed to influence lateral root defective phenotypes in phytomelatonin synthetic mutants of tomato.
- Taxon
- tomato (Solanum lycopersicum)Solanum lycopersicume0
- Material
- phytomelatonin synthetic mutantse5
- Treatment
- extra H2 supplye5
- Outcome
- LR defective phenotypese5
- Direction
- failed to influencee5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we discovered that AB increases tomato (Solanum lycopersicum) lateral root (LR) branching”
e5“Consistently, extra H2 supply failed to influence the LR defective phenotypes in phytomelatonin synthetic mutants”
Claim · model-written summary
Phytomelatonin-regulated auxin signaling and cell-cycle regulation are associated with AB/H2 control of lateral root branching.
- Taxon
- tomato (Solanum lycopersicum)Solanum lycopersicume0
- Treatment
- AB/H2e6
- Compound
- phytomelatonine6
- Outcome
- LR branchinge6
- Direction
- associatede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we discovered that AB increases tomato (Solanum lycopersicum) lateral root (LR) branching”
e6“Molecular evidence showed that the phytomelatonin-regulated auxin signaling network and cell-cycle regulation were associated with the AB/H2 control of LR branching”
Claim · model-written summary
AB and melatonin had little effect on lateral root branching in the presence of auxin synthetic inhibitors.
- Taxon
- tomato (Solanum lycopersicum)Solanum lycopersicume0
- Treatment
- AB and melatonine7
- Outcome
- LR branchinge7
- Direction
- had little effecte7
- Qualifier
- in the presence of auxin synthetic inhibitorse7
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“we discovered that AB increases tomato (Solanum lycopersicum) lateral root (LR) branching”
e7“Also, AB and melatonin had little effect on LR branching in the presence of auxin synthetic inhibitors”
The same questions in other papers
ammonia · growth · Solanum lycopersicum (tomato)Positive and negative results
1 in the opposite direction
- negative
melatonin · growth · Solanum lycopersicum (tomato)Positive and negative results
22 in the opposite direction
- positive
- positive
- positive
19 papers more in this group
See all 23 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.