Abstract
The article discusses the accelerated cultivation of quality planting material using the method of seed preparation by soaking in water treated in low-temperature plasma discharge. The most promising planting material are seedlings with closed root system. They are grown from improved seed grain in optimal conditions of greenhouses. Among various methods of stimulating germination of the seed germ, it is of interest to influence it with “cold” plasma. With the help of a specially designed device, which allows to treat water with lowtemperature plasma discharge, plasma-treated water for soaking seeds was obtained. The time of water treatment varied from 1 to 4 min. Water activated by plasma had acidity indicators other than those of ordinary water. The time of acidity level restoration was from 2 to 4 days. Germination rates and germination energy were obtained for seeds of Pinus silvestrys and Picea abies soaked in water with different duration of plasma treatment. When seeds were soaked in water treated with plasma for 1, 2 and 4 min, germination energy increased by 20–31 % for pine and by 6–18 % for spruce compared to the control (soaking in distilled water). Technical germination of pine seeds increased by 11–21 %, for spruce by 3–16 %. The results of research show changes in the structure and composition of the nutrient substance of the seed, in the structure of the germ and the degree of its development. Analysis of the chemical composition of seed endosperm a day after soaking relative to dry seeds showed an increase in its carbon content by 2–3 % and a decrease in oxygen by 1 %; potassium – by 0.5 %. When seeds were soaked in distilled water, the content of phosphorus in the endosperm decreased by 0.15 %, and the content of sodium – by 0.2 %. For seeds soaked in plasma treated water, phosphorus content decreased by 0.21–0.22 % and sodium content was not observed. Soaking seeds in plasma-treated water stimulates the processes of their preparation for germination and differentiation of germ tissues (formation of seed pods), compared to seeds soaked in water. For citation: Gavrilova O.I., Gostev K.V. Use of Plasma-treated Water to Prepare Seeds of Coniferous Species for Sowing. Lesnoy Zhurnal = Russian Forestry Journal, 2023, no. 5, pp. 204–211. (In Russ.). https://doi.org/10.37482/0536-1036-2023-5-204-211
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Claims 14
Claim · model-written summary
Soaking pine seed in plasma-treated water for 1, 2 and 4 min raised germination energy by 20–31%, relative to distilled water.
Verbatim
e0“seeds of Pinus silvestrys and Picea abies”
e1“When seeds were soaked in water treated with plasma for 1, 2 and 4 min, germination energy increased by 20–31 % for pine and by 6–18 % for spruce compared to the control (soaking in distilled water)”
Claim · model-written summary
Soaking spruce seed in plasma-treated water for 1, 2 and 4 min raised germination energy by 6–18%, relative to distilled water.
- Taxon
- Picea abies
Picea abiese0 - Treatment
- water treated with plasma for 1, 2 and 4 mine1
- Outcome
- germination energye1
- Direction
- increasede1
- Value
- 6–18 %e1
- Comparator
- soaking in distilled watere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“seeds of Pinus silvestrys and Picea abies”
e1“When seeds were soaked in water treated with plasma for 1, 2 and 4 min, germination energy increased by 20–31 % for pine and by 6–18 % for spruce compared to the control (soaking in distilled water)”
Claim · model-written summary
Soaking pine seed in plasma-treated water raised technical germination by 11–21%.
Verbatim
e0“seeds of Pinus silvestrys and Picea abies”
e1“When seeds were soaked in water treated with plasma for 1, 2 and 4 min, germination energy increased by 20–31 % for pine and by 6–18 % for spruce compared to the control (soaking in distilled water)”
e2“Technical germination of pine seeds increased by 11–21 %, for spruce by 3–16 %”
Claim · model-written summary
Soaking spruce seed in plasma-treated water raised technical germination by 3–16%.
- Taxon
- Picea abies
Picea abiese0 - Treatment
- water treated with plasma for 1, 2 and 4 mine1
- Outcome
- Technical germinatione2
- Direction
- increasede2
- Value
- 3–16 %e2
- Comparator
- soaking in distilled watere1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e0“seeds of Pinus silvestrys and Picea abies”
e1“When seeds were soaked in water treated with plasma for 1, 2 and 4 min, germination energy increased by 20–31 % for pine and by 6–18 % for spruce compared to the control (soaking in distilled water)”
e2“Technical germination of pine seeds increased by 11–21 %, for spruce by 3–16 %”
Claim · model-written summary
Endosperm carbon content of seed a day after soaking increased by 2–3% relative to dry seed.
Verbatim
e0“seeds of Pinus silvestrys and Picea abies”
e3“Analysis of the chemical composition of seed endosperm a day after soaking relative to dry seeds showed an increase in its carbon content by 2–3 % and a decrease in oxygen by 1 %; potassium – by 0.5 %”
Claim · model-written summary
Endosperm oxygen content of seed a day after soaking decreased by 1% relative to dry seed.
Verbatim
e0“seeds of Pinus silvestrys and Picea abies”
e3“Analysis of the chemical composition of seed endosperm a day after soaking relative to dry seeds showed an increase in its carbon content by 2–3 % and a decrease in oxygen by 1 %; potassium – by 0.5 %”
Claim · model-written summary
Endosperm potassium content of seed a day after soaking decreased by 0.5% relative to dry seed.
Verbatim
e0“seeds of Pinus silvestrys and Picea abies”
e3“Analysis of the chemical composition of seed endosperm a day after soaking relative to dry seeds showed an increase in its carbon content by 2–3 % and a decrease in oxygen by 1 %; potassium – by 0.5 %”
Claim · model-written summary
Soaking seed in distilled water decreased endosperm phosphorus content by 0.15%.
- Taxon
- Pinus silvestryse0
- Organ / stage
- endosperme4
- Treatment
- soaked in distilled watere4
- Outcome
- content of phosphoruse4
- Direction
- decreasede4
- Value
- 0.15 %e4
Not reportedComparator
Verbatim
e0“seeds of Pinus silvestrys and Picea abies”
e4“When seeds were soaked in distilled water, the content of phosphorus in the endosperm decreased by 0.15 %”
Claim · model-written summary
Soaking seed in distilled water decreased endosperm sodium content by 0.2%.
- Taxon
- Pinus silvestryse0
- Organ / stage
- endosperme4
- Treatment
- soaked in distilled watere4
- Outcome
- content of sodiume5
- Direction
- decreaseinferrede5
- Value
- 0.2 %e5
Not reportedComparator
Verbatim
e0“seeds of Pinus silvestrys and Picea abies”
e4“When seeds were soaked in distilled water, the content of phosphorus in the endosperm decreased by 0.15 %”
e5“and the content of sodium – by 0.2 %”
Claim · model-written summary
Soaking seed in plasma-treated water decreased endosperm phosphorus content by 0.21–0.22%.
- Taxon
- Pinus silvestryse0
- Treatment
- plasma treated watere6
- Outcome
- phosphorus contente6
- Direction
- decreasede6
- Value
- 0.21–0.22 %e6
Not reportedComparator
Verbatim
e0“seeds of Pinus silvestrys and Picea abies”
e6“For seeds soaked in plasma treated water, phosphorus content decreased by 0.21–0.22 % and sodium content was not observed”
Claim · model-written summary
Endosperm sodium content was not observed after soaking seed in plasma-treated water.
Verbatim
e0“seeds of Pinus silvestrys and Picea abies”
e6“For seeds soaked in plasma treated water, phosphorus content decreased by 0.21–0.22 % and sodium content was not observed”
Claim · model-written summary
Soaking seed in plasma-treated water stimulated the processes of preparation for germination and germ tissue differentiation, compared with seed soaked in water.
Verbatim
e0“seeds of Pinus silvestrys and Picea abies”
e7“Soaking seeds in plasma-treated water stimulates the processes of their preparation for germination and differentiation of germ tissues (formation of seed pods), compared to seeds soaked in water”
Claim · model-written summary
Plasma-activated water had acidity indicators different from ordinary water.
Not reportedTaxon
Verbatim
e1“Water activated by plasma had acidity indicators other than those of ordinary water”
Claim · model-written summary
Acidity of plasma-activated water was restored within 2 to 4 days.
Not reportedTaxon
Verbatim
e1“The time of acidity level restoration was from 2 to 4 days”