Paper · 2023 · Lesnoy Zhurnal (Forestry Journal)

Use of Plasma-treated Water to Prepare Seeds of Coniferous Species for Sowing

Olga I. Gavrilova; Kirill V. Gostev

14
claims
29
verbatim quotes
0
citations
4
research areas
✓
Open access · cc-by

Abstract

The article discusses the accelerated cultivation of quality planting material using the method of seed preparation by soaking in water treated in low-temperature plasma discharge. The most promising planting material are seedlings with closed root system. They are grown from improved seed grain in optimal conditions of greenhouses. Among various methods of stimulating germination of the seed germ, it is of interest to influence it with “cold” plasma. With the help of a specially designed device, which allows to treat water with lowtemperature plasma discharge, plasma-treated water for soaking seeds was obtained. The time of water treatment varied from 1 to 4 min. Water activated by plasma had acidity indicators other than those of ordinary water. The time of acidity level restoration was from 2 to 4 days. Germination rates and germination energy were obtained for seeds of Pinus silvestrys and Picea abies soaked in water with different duration of plasma treatment. When seeds were soaked in water treated with plasma for 1, 2 and 4 min, germination energy increased by 20–31 % for pine and by 6–18 % for spruce compared to the control (soaking in distilled water). Technical germination of pine seeds increased by 11–21 %, for spruce by 3–16 %. The results of research show changes in the structure and composition of the nutrient substance of the seed, in the structure of the germ and the degree of its development. Analysis of the chemical composition of seed endosperm a day after soaking relative to dry seeds showed an increase in its carbon content by 2–3 % and a decrease in oxygen by 1 %; potassium – by 0.5 %. When seeds were soaked in distilled water, the content of phosphorus in the endosperm decreased by 0.15 %, and the content of sodium – by 0.2 %. For seeds soaked in plasma treated water, phosphorus content decreased by 0.21–0.22 % and sodium content was not observed. Soaking seeds in plasma-treated water stimulates the processes of their preparation for germination and differentiation of germ tissues (formation of seed pods), compared to seeds soaked in water. For citation: Gavrilova O.I., Gostev K.V. Use of Plasma-treated Water to Prepare Seeds of Coniferous Species for Sowing. Lesnoy Zhurnal = Russian Forestry Journal, 2023, no. 5, pp. 204–211. (In Russ.). https://doi.org/10.37482/0536-1036-2023-5-204-211

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Claims 14

Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Soaking pine seed in plasma-treated water for 1, 2 and 4 min raised germination energy by 20–31%, relative to distilled water.

Taxon
Pinus silvestryse0
Treatment
water treated with plasma for 1, 2 and 4 mine1
Outcome
germination energye1
Direction
increasede1
Value
20–31 %e1
Comparator
soaking in distilled watere1

Verbatim

e0

“seeds of Pinus silvestrys and Picea abies”

✓ located verbatim in the source · abstract, characters 877–918 · show in text ↑
e1

“When seeds were soaked in water treated with plasma for 1, 2 and 4 min, germination energy increased by 20–31 % for pine and by 6–18 % for spruce compared to the control (soaking in distilled water)”

✓ located verbatim in the source · abstract, characters 980–1178 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Soaking spruce seed in plasma-treated water for 1, 2 and 4 min raised germination energy by 6–18%, relative to distilled water.

Taxon
Picea abiesPicea abiese0
Treatment
water treated with plasma for 1, 2 and 4 mine1
Outcome
germination energye1
Direction
increasede1
Value
6–18 %e1
Comparator
soaking in distilled watere1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Picea abies
Picea abies · TaxonIvar Leidus · CC BY-SA 3.0 ee · Wikimedia Commons ↗

Verbatim

e0

“seeds of Pinus silvestrys and Picea abies”

✓ located verbatim in the source · abstract, characters 877–918 · show in text ↑
e1

“When seeds were soaked in water treated with plasma for 1, 2 and 4 min, germination energy increased by 20–31 % for pine and by 6–18 % for spruce compared to the control (soaking in distilled water)”

✓ located verbatim in the source · abstract, characters 980–1178 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Soaking pine seed in plasma-treated water raised technical germination by 11–21%.

Taxon
Pinus silvestryse0
Treatment
water treated with plasma for 1, 2 and 4 mine1
Outcome
Technical germinatione2
Direction
increasede2
Value
11–21 %e2
Comparator
soaking in distilled watere1

Verbatim

e0

“seeds of Pinus silvestrys and Picea abies”

✓ located verbatim in the source · abstract, characters 877–918 · show in text ↑
e1

“When seeds were soaked in water treated with plasma for 1, 2 and 4 min, germination energy increased by 20–31 % for pine and by 6–18 % for spruce compared to the control (soaking in distilled water)”

✓ located verbatim in the source · abstract, characters 980–1178 · show in text ↑
e2

“Technical germination of pine seeds increased by 11–21 %, for spruce by 3–16 %”

✓ located verbatim in the source · abstract, characters 1180–1258 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Soaking spruce seed in plasma-treated water raised technical germination by 3–16%.

Taxon
Picea abiesPicea abiese0
Treatment
water treated with plasma for 1, 2 and 4 mine1
Outcome
Technical germinatione2
Direction
increasede2
Value
3–16 %e2
Comparator
soaking in distilled watere1

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Picea abies
Picea abies · TaxonIvar Leidus · CC BY-SA 3.0 ee · Wikimedia Commons ↗

Verbatim

e0

“seeds of Pinus silvestrys and Picea abies”

✓ located verbatim in the source · abstract, characters 877–918 · show in text ↑
e1

“When seeds were soaked in water treated with plasma for 1, 2 and 4 min, germination energy increased by 20–31 % for pine and by 6–18 % for spruce compared to the control (soaking in distilled water)”

✓ located verbatim in the source · abstract, characters 980–1178 · show in text ↑
e2

“Technical germination of pine seeds increased by 11–21 %, for spruce by 3–16 %”

✓ located verbatim in the source · abstract, characters 1180–1258 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Endosperm carbon content of seed a day after soaking increased by 2–3% relative to dry seed.

Taxon
Pinus silvestryse0
Organ / stage
seed endosperme3
Treatment
soakinge3
Outcome
carbon contente3
Direction
increasee3
Value
2–3 %e3
Comparator
dry seedse3

Verbatim

e0

“seeds of Pinus silvestrys and Picea abies”

✓ located verbatim in the source · abstract, characters 877–918 · show in text ↑
e3

“Analysis of the chemical composition of seed endosperm a day after soaking relative to dry seeds showed an increase in its carbon content by 2–3 % and a decrease in oxygen by 1 %; potassium – by 0.5 %”

✓ located verbatim in the source · abstract, characters 1433–1633 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Endosperm oxygen content of seed a day after soaking decreased by 1% relative to dry seed.

Taxon
Pinus silvestryse0
Organ / stage
seed endosperme3
Treatment
soakinge3
Outcome
oxygene3
Direction
decreasee3
Value
1 %e3
Comparator
dry seedse3

Verbatim

e0

“seeds of Pinus silvestrys and Picea abies”

✓ located verbatim in the source · abstract, characters 877–918 · show in text ↑
e3

“Analysis of the chemical composition of seed endosperm a day after soaking relative to dry seeds showed an increase in its carbon content by 2–3 % and a decrease in oxygen by 1 %; potassium – by 0.5 %”

✓ located verbatim in the source · abstract, characters 1433–1633 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Endosperm potassium content of seed a day after soaking decreased by 0.5% relative to dry seed.

Taxon
Pinus silvestryse0
Organ / stage
seed endosperme3
Treatment
soakinge3
Outcome
potassiume3
Direction
decreaseinferrede3
Value
0.5 %e3
Comparator
dry seedse3

Verbatim

e0

“seeds of Pinus silvestrys and Picea abies”

✓ located verbatim in the source · abstract, characters 877–918 · show in text ↑
e3

“Analysis of the chemical composition of seed endosperm a day after soaking relative to dry seeds showed an increase in its carbon content by 2–3 % and a decrease in oxygen by 1 %; potassium – by 0.5 %”

✓ located verbatim in the source · abstract, characters 1433–1633 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Soaking seed in distilled water decreased endosperm phosphorus content by 0.15%.

Taxon
Pinus silvestryse0
Organ / stage
endosperme4
Treatment
soaked in distilled watere4
Outcome
content of phosphoruse4
Direction
decreasede4
Value
0.15 %e4

Not reportedComparator

Verbatim

e0

“seeds of Pinus silvestrys and Picea abies”

✓ located verbatim in the source · abstract, characters 877–918 · show in text ↑
e4

“When seeds were soaked in distilled water, the content of phosphorus in the endosperm decreased by 0.15 %”

✓ located verbatim in the source · abstract, characters 1635–1740 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Soaking seed in distilled water decreased endosperm sodium content by 0.2%.

Taxon
Pinus silvestryse0
Organ / stage
endosperme4
Treatment
soaked in distilled watere4
Outcome
content of sodiume5
Direction
decreaseinferrede5
Value
0.2 %e5

Not reportedComparator

Verbatim

e0

“seeds of Pinus silvestrys and Picea abies”

✓ located verbatim in the source · abstract, characters 877–918 · show in text ↑
e4

“When seeds were soaked in distilled water, the content of phosphorus in the endosperm decreased by 0.15 %”

✓ located verbatim in the source · abstract, characters 1635–1740 · show in text ↑
e5

“and the content of sodium – by 0.2 %”

✓ located verbatim in the source · abstract, characters 1742–1778 · show in text ↑
Intervention effectobservedoriginal resultmeasured

Claim · model-written summary

Soaking seed in plasma-treated water decreased endosperm phosphorus content by 0.21–0.22%.

Taxon
Pinus silvestryse0
Treatment
plasma treated watere6
Outcome
phosphorus contente6
Direction
decreasede6
Value
0.21–0.22 %e6

Not reportedComparator

Verbatim

e0

“seeds of Pinus silvestrys and Picea abies”

✓ located verbatim in the source · abstract, characters 877–918 · show in text ↑
e6

“For seeds soaked in plasma treated water, phosphorus content decreased by 0.21–0.22 % and sodium content was not observed”

✓ located verbatim in the source · abstract, characters 1780–1901 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Endosperm sodium content was not observed after soaking seed in plasma-treated water.

Taxon
Pinus silvestryse0
Outcome
sodium contente6
Qualifier
was not observede6

Verbatim

e0

“seeds of Pinus silvestrys and Picea abies”

✓ located verbatim in the source · abstract, characters 877–918 · show in text ↑
e6

“For seeds soaked in plasma treated water, phosphorus content decreased by 0.21–0.22 % and sodium content was not observed”

✓ located verbatim in the source · abstract, characters 1780–1901 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Soaking seed in plasma-treated water stimulated the processes of preparation for germination and germ tissue differentiation, compared with seed soaked in water.

Taxon
Pinus silvestryse0
Organ / stage
germ tissuese7
Treatment
Soaking seeds in plasma-treated watere7
Outcome
processes of their preparation for germinatione7
Direction
stimulatese7
Comparator
seeds soaked in watere7

Verbatim

e0

“seeds of Pinus silvestrys and Picea abies”

✓ located verbatim in the source · abstract, characters 877–918 · show in text ↑
e7

“Soaking seeds in plasma-treated water stimulates the processes of their preparation for germination and differentiation of germ tissues (formation of seed pods), compared to seeds soaked in water”

✓ located verbatim in the source · abstract, characters 1903–2098 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Plasma-activated water had acidity indicators different from ordinary water.

Outcome
acidity indicatorse1
Comparator
ordinary watere1

Not reportedTaxon

Verbatim

e1

“Water activated by plasma had acidity indicators other than those of ordinary water”

✓ located verbatim in the source · abstract, characters 673–756 · show in text ↑

schema checkedfield values found in the cited quotesPermalinkReport an issue

Observationobservedoriginal resultmeasured

Claim · model-written summary

Acidity of plasma-activated water was restored within 2 to 4 days.

Outcome
time of acidity level restoratione1
Value
2 to 4 dayse1

Not reportedTaxon

Verbatim

e1

“The time of acidity level restoration was from 2 to 4 days”

✓ located verbatim in the source · abstract, characters 758–816 · show in text ↑

schema checkedfield values found in the cited quotesPermalinkReport an issue