Abstract
OBJECTIVE: Grape hybrids are characterized by different chemical compositions; often with high hybrids are characterized by different chemical compositions, often with a high phenolic content and a specific profile of anthocyanins. The aim of study was to characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts and their influence on apoptosis induction and antiproliferative effects on human prostate cancer cells. METHODS: We used the MTT assay to evaluate the cytotoxic effect of extracts of SA and SU, on the prostate adenocarcinoma cell lines PC-3 and DU-145. To analyze the inhibiting impact by flow cytometry, used 24 and 48 hours. Anthocyanins were quantified by liquid chromatography and analysed by their absorption rate, hepatotoxicity, blood concentration, blood-brain barrier passage ability and maximum recommended dose by in silico approaches. RESULTS: Our results showed that malvidin derivatives present the highest content in both cultivars. We identified 14.46mg/100g malvidin-3-O-glycoside in SA and 2.76 mg/100 g in SU. A reduction in cell viability of DU-145 (45 and 65%) and PC-3 (63 and 67%) cells after 48h treatment with SA and SU, respectively, was found via MTT assay. Flow cytometry showed that the treatment with extracts from SA and SU had an inhibitory impact on cell development due to G2/M arrest and caused a rise in apoptotic cells compared to control group. None of the anthocyanin presented hepatotoxicity as well as blood-brain barrier passage ability. Peonidin 3-O-glucoside had the lower maximum recommended dose as well as the highest intestinal absorption rate. However, delphinidin 3-O-glucoside had the highest blood concentration values. CONCLUSION: The findings of this study highlight the potential of hybrid Vitis vinifera L. varieties as an important source of natural antioxidants and their protective effect against prostate cancer cells as well as elucidate in part their anthocyanin's metabolism.
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Claims 13
Claim · model-written summary
Malvidin derivatives had the highest content in both grape cultivars.
- Taxon
- Vitis vinifera
Vitis viniferae0 - Outcome
- malvidin derivativese1
- Direction
- higheste1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts”
e1“malvidin derivatives present the highest content in both cultivars”
Claim · model-written summary
Sweet Sapphire contained 14.46 mg/100 g malvidin-3-O-glycoside.
- Taxon
- Vitis viniferaVitis viniferae0
- Material
- SAe2
- Outcome
- malvidin-3-O-glycosidee2
- Value
- 14.46mg/100ge2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts”
e2“We identified 14.46mg/100g malvidin-3-O-glycoside in SA and 2.76 mg/100 g in SU”
Claim · model-written summary
Sweet Surprise contained 2.76 mg/100 g malvidin-3-O-glycoside.
- Taxon
- Vitis viniferaVitis viniferae0
- Material
- SUe2
- Outcome
- malvidin-3-O-glycosidee2
- Value
- 2.76 mg/100 ge2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts”
e2“We identified 14.46mg/100g malvidin-3-O-glycoside in SA and 2.76 mg/100 g in SU”
Claim · model-written summary
SA extract reduced DU-145 prostate cancer cell viability by 45%.
- Taxon
- Vitis viniferaVitis viniferae0
- Material
- DU-145e3
- Treatment
- treatment with SA and SUe3
- Outcome
- cell viabilitye3
- Direction
- reductione3
- Value
- 45e3
- Qualifier
- SAe3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts”
e3“A reduction in cell viability of DU-145 (45 and 65%) and PC-3 (63 and 67%) cells after 48h treatment with SA and SU, respectively, was found via MTT assay”
Claim · model-written summary
SU extract reduced DU-145 prostate cancer cell viability by 65%.
- Taxon
- Vitis viniferaVitis viniferae0
- Material
- DU-145e3
- Treatment
- treatment with SA and SUe3
- Outcome
- cell viabilitye3
- Direction
- reductione3
- Value
- 65e3
- Qualifier
- SUe3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts”
e3“A reduction in cell viability of DU-145 (45 and 65%) and PC-3 (63 and 67%) cells after 48h treatment with SA and SU, respectively, was found via MTT assay”
Claim · model-written summary
SA extract reduced PC-3 prostate cancer cell viability by 63%.
- Taxon
- Vitis viniferaVitis viniferae0
- Material
- PC-3e3
- Treatment
- treatment with SA and SUe3
- Outcome
- cell viabilitye3
- Direction
- reductione3
- Value
- 63e3
- Qualifier
- SAe3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts”
e3“A reduction in cell viability of DU-145 (45 and 65%) and PC-3 (63 and 67%) cells after 48h treatment with SA and SU, respectively, was found via MTT assay”
Claim · model-written summary
SU extract reduced PC-3 prostate cancer cell viability by 67%.
- Taxon
- Vitis viniferaVitis viniferae0
- Material
- PC-3e3
- Treatment
- treatment with SA and SUe3
- Outcome
- cell viabilitye3
- Direction
- reductione3
- Value
- 67e3
- Qualifier
- SUe3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts”
e3“A reduction in cell viability of DU-145 (45 and 65%) and PC-3 (63 and 67%) cells after 48h treatment with SA and SU, respectively, was found via MTT assay”
Claim · model-written summary
Extracts of SA and SU had an inhibitory impact on prostate cancer cell development due to G2/M arrest.
- Taxon
- Vitis viniferaVitis viniferae0
- Treatment
- treatment with extracts from SA and SUe4
- Outcome
- cell developmente4
- Direction
- inhibitory impacte4
- Qualifier
- G2/M arreste4
- Comparator
- control groupe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts”
e4“Flow cytometry showed that the treatment with extracts from SA and SU had an inhibitory impact on cell development due to G2/M arrest and caused a rise in apoptotic cells compared to control group”
Claim · model-written summary
Extracts of SA and SU caused a rise in apoptotic cells compared with the control group.
- Taxon
- Vitis viniferaVitis viniferae0
- Treatment
- treatment with extracts from SA and SUe4
- Outcome
- apoptotic cellse4
- Direction
- risee4
- Comparator
- control groupe4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts”
e4“Flow cytometry showed that the treatment with extracts from SA and SU had an inhibitory impact on cell development due to G2/M arrest and caused a rise in apoptotic cells compared to control group”
Claim · model-written summary
In silico, none of the anthocyanins was predicted to be hepatotoxic or to pass the blood-brain barrier.
- Taxon
- Vitis viniferaVitis viniferae0
- Compound
- anthocyanine5
- Outcome
- hepatotoxicity as well as blood-brain barrier passage abilitye5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts”
e5“None of the anthocyanin presented hepatotoxicity as well as blood-brain barrier passage ability”
Claim · model-written summary
Peonidin 3-O-glucoside had the lower in silico maximum recommended dose.
- Taxon
- Vitis viniferaVitis viniferae0
- Compound
- Peonidin 3-O-glucosidee6
- Outcome
- maximum recommended dosee6
- Direction
- lowere6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts”
e6“Peonidin 3-O-glucoside had the lower maximum recommended dose as well as the highest intestinal absorption rate”
Claim · model-written summary
Peonidin 3-O-glucoside had the highest in silico intestinal absorption rate.
- Taxon
- Vitis viniferaVitis viniferae0
- Compound
- Peonidin 3-O-glucosidee6
- Outcome
- intestinal absorption ratee6
- Direction
- higheste6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts”
e6“Peonidin 3-O-glucoside had the lower maximum recommended dose as well as the highest intestinal absorption rate”
Claim · model-written summary
Delphinidin 3-O-glucoside had the highest in silico blood concentration values.
- Taxon
- Vitis viniferaVitis viniferae0
- Compound
- delphinidin 3-O-glucosidee7
- Outcome
- blood concentration valuese7
- Direction
- higheste7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“characterize the constituents of hybrid Vitis vinifera L. varieties Sweet Sapphire (SA) and Sweet Surprise (SU) extracts”
e7“However, delphinidin 3-O-glucoside had the highest blood concentration values”
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