Abstract
Pepper plants (Capsicum annuum) with severe leaf curl symptoms were collected in 2013 from Bangalore, Karnataka, India. The detection results showed a co-infection between the tomato leaf curl Joydebpur virus (ToLCJoV) and tomato leaf curl Bangladesh betasatellite (ToLCBDB) through the sequencing analysis of PCR amplicons. To pinpoint the molecular mechanism of this uncommon combination, infectious clones of ToLCJoV and two different betasatellites—ToLCBDB and tomato leaf curl Joydebpur betasatellite (ToLCJoB)—were constructed and tested for their infectivity in Nicotiana benthamiana. Together, we conducted various combined agroinoculation studies to compare the interaction of ToLCJoV with non-cognate and cognate betasatellites. The natural non-cognate interaction between ToLCJoV and ToLCBDB showed severe symptoms compared to the mild symptoms of a cognate combination (ToLCJoV × ToLCJoB) in infected plants. A sequence comparison among betasatellites and their helper virus wasperformed and the iteron resemblances in ToLCBDB as well as ToLCJoB clones were processed. Mutant betasatellites that comprised iteron modifications revealed that changes in iteron sequences could disturb the transreplication process between betasatellites and their helper virus. Our study might provide an important consideration for determining the efficiency of transreplication activity between betasatellites and their helper virus.
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Claims 2
Claim · model-written summary
The non-cognate interaction between ToLCJoV and ToLCBDB caused more severe symptoms than the cognate combination.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae1 - Treatment
- non-cognate interaction between ToLCJoV and ToLCBDBe2
- Interactor
- ToLCJoVe2pathogen
- Second interactor
- ToLCBDBe2other
- Outcome
- symptomse2
- Direction
- severee2
- Comparator
- cognate combination (ToLCJoV × ToLCJoB)e2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“tested for their infectivity in Nicotiana benthamiana”
e2“The natural non-cognate interaction between ToLCJoV and ToLCBDB showed severe symptoms compared to the mild symptoms of a cognate combination (ToLCJoV × ToLCJoB) in infected plants”
Claim · model-written summary
Iteron modifications in mutant betasatellites disturb transreplication between betasatellites and their helper virus.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Treatment
- iteron modificationse3
- Interactor
- helper viruse3pathogen
- Second interactor
- betasatellitesBetasatellitee3other
- Outcome
- transreplication processe3
- Direction
- disturbe3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“tested for their infectivity in Nicotiana benthamiana”
e3“Mutant betasatellites that comprised iteron modifications revealed that changes in iteron sequences could disturb the transreplication process between betasatellites and their helper virus”
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Solanum lycopersicum · disease · Nicotiana benthamianaResults agree
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.