Abstract
Expression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 protein and green fluorescent protein (GFP) fused to the C2 protein (C2-GFP) in Nicotiana benthamiana from a Potato virus X (PVX) vector induced necrotic ringspots on inoculated leaves as well as necrotic vein banding and severe necrosis on systemically infected leaves. The localization of GFP fluorescence in plant cells infected with PVX/C2-GFP and in insect cells transfected with Baculovirus expressing C2-GFP indicates that the TYLCV-C C2 protein is capable of shuttling GFP into plant and insect cell nuclei. Our data demonstrate that the TYLCV-C C2 protein may contribute to viral pathogenicity in planta and is nuclear localized.
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Claims 3
Claim · model-written summary
C2 expression induced necrotic ringspots on inoculated leaves.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Expression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 protein and green fluorescent protein (GFP) fused to the C2 protein (C2-GFP)e0
- Interactor
- Tomato yellow leaf curl virus-Chinae0pathogen
- Gene
- TYLCV-C) C2 proteine0
- Outcome
- necrotic ringspots on inoculated leavese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 protein and green fluorescent protein (GFP) fused to the C2 protein (C2-GFP) in Nicotiana benthamiana from a Potato virus X (PVX) vector induced necrotic ringspots on inoculated leaves”
Claim · model-written summary
C2 expression induced necrotic vein banding and severe necrosis on systemic leaves.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae0
- Treatment
- Expression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 proteine0
- Interactor
- Tomato yellow leaf curl virus-Chinae0pathogen
- Outcome
- necrotic vein banding and severe necrosis on systemically infected leavese0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression of the Tomato yellow leaf curl virus-China (TYLCV-C) C2 protein and green fluorescent protein (GFP) fused to the C2 protein (C2-GFP) in Nicotiana benthamiana from a Potato virus X (PVX) vector induced necrotic ringspots on inoculated leaves as well as necrotic vein banding and severe necrosis on systemically infected leaves”
Claim · model-written summary
C2 protein shuttled GFP into plant cell nuclei.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae1
- Interactor
- PVXPotato virus Xe0pathogen
- Gene
- TYLCV-C C2 proteine0
- Outcome
- shuttling GFP into plant and insect cell nucleie0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“localization of GFP fluorescence in plant cells infected with PVX/C2-GFP and in insect cells transfected with Baculovirus expressing C2-GFP indicates that the TYLCV-C C2 protein is capable of shuttling GFP into plant and insect cell nuclei”
e1“Nicotiana benthamiana”
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