Abstract
The activation of an immune response in tomato (Solanum lycopersicum) against Pseudomonas syringae relies on the recognition of E3 ligase-deficient forms of AvrPtoB by the host protein kinase, Fen. To investigate the mechanisms by which Fen-mediated immunity is regulated, we characterize in this study a Fen-interacting protein, Fni3, and its cofactor, S. lycoperiscum Uev (Suv). Fni3 encodes a homolog of the Ubc13-type ubiquitin-conjugating enzyme that catalyzes exclusively Lys-63-linked ubiquitination, whereas Suv is a ubiquitin-conjugating enzyme variant. The C-terminal region of Fen was necessary for interaction with Fni3, and this interaction was required for cell death triggered by overexpression of Fen in Nicotiana benthamiana leaves. Fni3 was shown to be an active E2 enzyme, but Suv displayed no ubiquitin-conjugating activity; Fni3 and Suv together directed Lys-63-linked ubiquitination. Decreased expression of Fni3, another tomato Ubc13 homolog, Sl-Ubc13-2, or Suv in N. benthamiana leaves diminished cell death associated with Fen-mediated immunity and cell death elicited by several other resistance (R) proteins and their cognate effectors. We also discovered that coexpression of Fen and other R proteins/effectors with a Fni3 mutant that is compromised for ubiquitin-conjugating activity diminished the cell death. These results suggest that Fni3/Sl-Ubc13-2 and Suv regulate the immune response mediated by Fen and other R proteins through Lys-63-linked ubiquitination.
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Claims 12
Claim · model-written summary
Fni3 encodes a Ubc13-type enzyme catalyzing Lys-63-linked ubiquitination.
- Taxon
- tomato
Solanum lycopersicume1 - Gene
- Fni3e0
- Outcome
- Lys-63-linked ubiquitinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fni3 encodes a homolog of the Ubc13-type ubiquitin-conjugating enzyme that catalyzes exclusively Lys-63-linked ubiquitination”
e1“tomato (Solanum lycopersicum)”
Claim · model-written summary
Suv is a ubiquitin-conjugating enzyme variant.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- Suve0
- Outcome
- ubiquitin-conjugating enzyme variante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suv is a ubiquitin-conjugating enzyme variant”
e1“tomato (Solanum lycopersicum)”
Claim · model-written summary
The Fen C-terminal region was needed for Fni3 interaction.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- Fene0
- Outcome
- interaction with Fni3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The C-terminal region of Fen was necessary for interaction with Fni3”
e1“tomato (Solanum lycopersicum)”
Claim · model-written summary
Fen-Fni3 interaction was required for Fen-overexpression-triggered cell death in Nicotiana leaves.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Gene
- Fene0
- Outcome
- cell deathe0
- Qualifier
- this interactione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“this interaction was required for cell death triggered by overexpression of Fen in Nicotiana benthamiana leaves”
Claim · model-written summary
Fni3 showed active E2 enzyme activity.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- Fni3e0
- Outcome
- active E2 enzymee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fni3 was shown to be an active E2 enzyme”
e1“tomato (Solanum lycopersicum)”
Claim · model-written summary
Suv showed no ubiquitin-conjugating activity.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- Suve0
- Outcome
- no ubiquitin-conjugating activitye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Suv displayed no ubiquitin-conjugating activity”
e1“tomato (Solanum lycopersicum)”
Claim · model-written summary
Fni3 and Suv together directed Lys-63-linked ubiquitination.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- Fni3 and Suve0
- Outcome
- Lys-63-linked ubiquitinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fni3 and Suv together directed Lys-63-linked ubiquitination”
e1“tomato (Solanum lycopersicum)”
Claim · model-written summary
Reduced Fni3 expression diminished Fen-mediated and other R-protein-associated cell death in Nicotiana leaves.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- Decreased expression of Fni3e0
- Gene
- Fni3e0
- Outcome
- cell death associated with Fen-mediated immunity and cell death elicited by several other resistance (R) proteins and their cognate effectorse0
- Direction
- diminishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Decreased expression of Fni3, another tomato Ubc13 homolog, Sl-Ubc13-2, or Suv in N. benthamiana leaves diminished cell death associated with Fen-mediated immunity and cell death elicited by several other resistance (R) proteins and their cognate effectors”
Claim · model-written summary
Reduced Sl-Ubc13-2 expression diminished Fen-mediated and other R-protein-associated cell death in Nicotiana leaves.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- Decreased expression of Fni3, another tomato Ubc13 homolog, Sl-Ubc13-2e0
- Gene
- Sl-Ubc13-2e0
- Outcome
- cell death associated with Fen-mediated immunity and cell death elicited by several other resistance (R) proteins and their cognate effectorse0
- Direction
- diminishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Decreased expression of Fni3, another tomato Ubc13 homolog, Sl-Ubc13-2, or Suv in N. benthamiana leaves diminished cell death associated with Fen-mediated immunity and cell death elicited by several other resistance (R) proteins and their cognate effectors”
Claim · model-written summary
Reduced Suv expression diminished Fen-mediated and other R-protein-associated cell death in Nicotiana leaves.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Treatment
- Suve0
- Gene
- Suve0
- Outcome
- cell death associated with Fen-mediated immunity and cell death elicited by several other resistance (R) proteins and their cognate effectorse0
- Direction
- diminishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Decreased expression of Fni3, another tomato Ubc13 homolog, Sl-Ubc13-2, or Suv in N. benthamiana leaves diminished cell death associated with Fen-mediated immunity and cell death elicited by several other resistance (R) proteins and their cognate effectors”
Claim · model-written summary
Coexpression with conjugation-compromised Fni3 diminished Fen- and other R-protein-associated cell death.
- Taxon
- tomatoSolanum lycopersicume1
- Treatment
- coexpression of Fen and other R proteins/effectors with a Fni3 mutante0
- Gene
- Fni3e0
- Outcome
- cell deathe0
- Direction
- diminishede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“coexpression of Fen and other R proteins/effectors with a Fni3 mutant that is compromised for ubiquitin-conjugating activity diminished the cell death”
e1“tomato (Solanum lycopersicum)”
Claim · model-written summary
Authors infer Fni3, Sl-Ubc13-2, and Suv regulate Fen and other R-protein immunity through Lys-63-linked ubiquitination.
- Taxon
- tomatoSolanum lycopersicume1
- Gene
- Fni3/Sl-Ubc13-2 and Suve0
- Outcome
- immune responsee0
- Qualifier
- through Lys-63-linked ubiquitinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fni3/Sl-Ubc13-2 and Suv regulate the immune response mediated by Fen and other R proteins through Lys-63-linked ubiquitination”
e1“tomato (Solanum lycopersicum)”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.