Abstract
A-galactosidases (AGALs), the oligosaccharide (RFO) catabolic genes of the raffinose family, play crucial roles in plant growth and development and in adversity stress. They can break down the non-reducing terminal galactose residues of glycolipids and sugar chains. In this study, the whole genome of AGALs was analyzed. Bioinformatics analysis was conducted to analyze members of the AGAL family in Gossypium hirsutum , Gossypium arboreum , Gossypium barbadense , and Gossypium raimondii. Meanwhile, RT-qPCR was carried out to analyze the expression patterns of AGAL family members in different tissues of terrestrial cotton. It was found that a series of environmental factors stimulated the expression of the GhAGAL3 gene. The function of GhAGAL3 was verified through virus-induced gene silencing (VIGS). As a result, GhAGAL3 gene silencing resulted in milder wilting of seedlings than the controls, and a significant increase in the raffinose content in cotton, indicating that GhAGAL3 responded to NaCl stress. The increase in raffinose content improved the tolerance of cotton. Findings in this study lay an important foundation for further research on the role of the GhAGAL3 gene family in the molecular mechanism of abiotic stress resistance in cotton.
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Claims 4
Claim · model-written summary
Environmental factors stimulated GhAGAL3 expression.
- Taxon
- cotton
Gossypium hirsutumt0 - Treatment
- a series of environmental factorse0
- Gene
- GhAGAL3e0
- Outcome
- expression of the GhAGAL3 genee0
- Direction
- stimulatede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a series of environmental factors stimulated the expression of the GhAGAL3 gene”
t0“terrestrial cotton”
Claim · model-written summary
GhAGAL3 silencing caused milder seedling wilting than controls.
- Taxon
- cottonGossypium hirsutumt0
- Treatment
- GhAGAL3 gene silencinge0
- Gene
- GhAGAL3e0
- Outcome
- wilting of seedlingse0
- Direction
- mildere0
- Comparator
- the controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhAGAL3 gene silencing resulted in milder wilting of seedlings than the controls”
t0“terrestrial cotton”
Claim · model-written summary
GhAGAL3 silencing significantly increased cotton raffinose content.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- GhAGAL3 gene silencinge0
- Gene
- GhAGAL3e0
- Outcome
- raffinose contente0
- Direction
- increasee0
- Comparator
- the controlse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“GhAGAL3 gene silencing resulted in milder wilting of seedlings than the controls, and a significant increase in the raffinose content in cotton”
Claim · model-written summary
The authors attribute improved cotton tolerance to increased raffinose content.
- Taxon
- cottonGossypium hirsutume0
- Compound
- raffinosee0
- Outcome
- tolerance of cottone0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase in raffinose content improved the tolerance of cotton”
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