Abstract
Plant breeding programs need to be carried out in order to improve the genetics of wheat that is able to adapt to tropical environments through hybridization, mutation induction, tissue culture, and genetic transformation. In vitro culture through somatic embryogenesis pathways plays an important role in genetic improvement and its integration with other breeding programs can positively affect the improvement of wheat quality, quantity, and development in Indonesia. The purpose of this study was to obtain an embryogenic callus induction method from the Dewata variety using five different types of explants, namely mature Seeds, immature embryos, immature seeds, leaf, stem, and to obtain combination of plant growth regulators and putrescine on somatic embryogenesis of wheat. The experimental design was prepared based on a complete randomized design with a combination treatment of embryogenic callus induction media consisting of 9 levels, namely: 1 ppm 2.4- D, 1 ppm 2.4-D + 1 ppm Picloram, ppm 2.4-D + 1 mg / L Picloram, 1 ppm 2.4-D + 10-4 M Putrescine, 1 ppm 2.4-D + 1 ppm Picloram +10-4 M Putrescine, 2 ppm 2.4-D + 1 ppm Picloram + 10-4 M Putrescine, 1 ppm 2.4 D + 10-3 M Putrescine, 1 ppm 2.4 D + 1 ppm Picloram + 10-3 M Putrescine, 2 ppm 2.4 D + 1 ppm Picloram + 10-3 M Putrescine. The results showed that the media used was able to induce embryogenic callus using mature seed and immature embryo, but immature seed and leaf were not able to produce embryogenic callus. The best media that produced the highest percentage of embryogenic callus was 2 ppm 2.4-D + 1 ppm Picloram + 10-4 M Putrescine with as much as 85.9% in young embryo explants.
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Claims 3
Claim · model-written summary
The tested media induced embryogenic callus from mature seed and immature embryo explants of the Dewata wheat variety.
- Taxon
- Wheat
Triticum aestivumt0 - Material
- Dewata varietye3
- Organ / stage
- mature seed and immature embryoe1
- Outcome
- induce embryogenic calluse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat (Triticum Aestivum L.)”
e1“The results showed that the media used was able to induce embryogenic callus using mature seed and immature embryo, but immature seed and leaf were not able to produce embryogenic callus”
e3“obtain an embryogenic callus induction method from the Dewata variety”
Claim · model-written summary
Immature seed and leaf explants of the Dewata wheat variety did not produce embryogenic callus.
- Taxon
- WheatTriticum aestivumt0
- Material
- Dewata varietye3
- Organ / stage
- immature seed and leafe1
- Outcome
- produce embryogenic calluse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat (Triticum Aestivum L.)”
e1“The results showed that the media used was able to induce embryogenic callus using mature seed and immature embryo, but immature seed and leaf were not able to produce embryogenic callus”
e3“obtain an embryogenic callus induction method from the Dewata variety”
Claim · model-written summary
The medium with 2 ppm 2.4-D, 1 ppm Picloram and 10-4 M putrescine gave the highest percentage of embryogenic callus, 85.9%, in young embryo explants.
- Taxon
- WheatTriticum aestivumt0
- Organ / stage
- young embryo explantse4
- Treatment
- 2 ppm 2.4-D + 1 ppm Picloram + 10-4 M Putrescinee4
- Outcome
- percentage of embryogenic calluse4
- Direction
- higheste4
- Value
- 85.9%e4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Wheat (Triticum Aestivum L.)”
e4“The best media that produced the highest percentage of embryogenic callus was 2 ppm 2.4-D + 1 ppm Picloram + 10-4 M Putrescine with as much as 85.9% in young embryo explants”
The same questions in other papers
putrescine · growth · Triticum aestivum (wheat)Results differ
12 papers in the same direction as this paper · 1 reporting no difference from an alternative
- positive
- no effect
- positive
10 papers more in this group
See all 14 papers →picloram · growth · Triticum aestivum (wheat)Positive and negative results
2 papers in the same direction as this paper · 1 in the opposite direction
- negative
- positive
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.