Abstract
Seed weight is an important target trait in pomegranate breeding and culture. Expansins act by loosening plant cell walls and cellulosic materials, permitting turgor-driven cell enlargement. However, the role of expansin genes (EXPs) in pomegranate seed weight remains elusive. A total of 29 PgrEXPs were identified in the ‘Dabenzi’ genome. These genes were classified into four subfamilies and 14 subgroups, including 22 PgrEXPAs, 5 PgrEXPBs, 1 PgrEXPLA, and 1 PgrEXPLB. Transcriptome analysis of PgrEXPs in different tissues (root, leaf, flower, peel, and seed testa) in ‘Dabenzi’, and the seed testa of the hard-seeded pomegranate cultivar ‘Dabenzi’ and soft-seeded cultivar ‘Tunisia’ at three development stages showed that three PgrEXPs (PgrEXPA11, PgrEXPA22, PgrEXPA6) were highly expressed throughout seed development, especially in the sarcotesta. SNP/Indel markers of these PgrEXPs were developed and used to genotype 101 pomegranate accessions. The association of polymorphic PgrEXPs with seed weight-related traits (100-seed weight, 100-kernel weight, 100-sarcotesta weight, and the percentage of 100-sarcotesta to 100-seed weight) were analyzed. PgrEXP22 was significantly associated with 100-seed weight and 100-sarcotesta weight and is a likely candidate for regulating seed weight and sarcotesta development in particular. This study provides an effective tool for the genetic improvement of seed weight in pomegranate breeding programs.
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Claims 5
Claim · model-written summary
PgrEXPA11, PgrEXPA22 and PgrEXPA6 were highly expressed throughout seed development, especially in the sarcotesta.
- Taxon
- pomegranate
Punica granatume0 - Organ / stage
- sarcotestae1
- Gene
- PgrEXPA11, PgrEXPA22, PgrEXPA6e1
- Outcome
- highly expressed throughout seed developmente1
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seed weight is an important target trait in pomegranate breeding”
e1“three PgrEXPs (PgrEXPA11, PgrEXPA22, PgrEXPA6) were highly expressed throughout seed development, especially in the sarcotesta”
Claim · model-written summary
A total of 29 PgrEXP genes were identified in the Dabenzi genome.
- Taxon
- Punica granatumPunica granatumt0
- Outcome
- 29 PgrEXPs were identifiede2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate (Punica granatum L.)”
e2“A total of 29 PgrEXPs were identified in the”
Claim · model-written summary
The PgrEXP genes were classified into four subfamilies and 14 subgroups.
- Taxon
- Punica granatumPunica granatumt0
- Outcome
- classified into four subfamilies and 14 subgroupse3
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Pomegranate (Punica granatum L.)”
e3“These genes were classified into four subfamilies and 14 subgroups”
Claim · model-written summary
PgrEXP22 was significantly associated with 100-seed weight and 100-sarcotesta weight in pomegranate accessions.
- Taxon
- pomegranatePunica granatume0
- Gene
- PgrEXP22e4
- Outcome
- 100-seed weight and 100-sarcotesta weighte4
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seed weight is an important target trait in pomegranate breeding”
e4“PgrEXP22 was significantly associated with 100-seed weight and 100-sarcotesta weight”
Claim · model-written summary
PgrEXP22 is a likely candidate for regulating seed weight and sarcotesta development in pomegranate.
- Taxon
- pomegranatePunica granatume0
- Gene
- PgrEXP22e4
- Outcome
- seed weight and sarcotesta developmente5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Seed weight is an important target trait in pomegranate breeding”
e4“PgrEXP22 was significantly associated with 100-seed weight and 100-sarcotesta weight”
e5“is a likely candidate for regulating seed weight and sarcotesta development”