Abstract
This study was investigate the effect of components media and silver nanoparticles (Ag-NPs) synthesized in the laboratory on the induction of biomass and secondary metabolites of calli cultures induced from the cotyledons of Trigonella foenum-graecum L. The culture media LS and B5 were tested with the growth regulators 2,4-Dichlorophenoxyacetic acid (2,4-D) at a concentration of 1.0, 2.0 and 3.0 mg l-1 and 6-benzyladenine (BA) at a concentration of 0.0, 0.5, 1.0, 1.5 and 2.0 mg l-1, with the aim of studying its effect on callus induction. In comparison, Ag-NPs were used at a concentration of 0.0, 0.5, 1.0, 1.5 and 2.0 mg l-1 to study its effect on the production of some secondary compounds. The results showed that the mixture consisting of the culture media LS with 3.0 mg l-1 of 2,4-D and 1.0 mg L-1 of BA the highest percentage of callus induction and the minimum days to complete callus formation. While, the same media with 2.0 mg l-1 2,4-D and 1.0 mg l-1 of BA recorded the highest dry weight (DW) and relative water content (RWC). The combination B5×2.0 mg l-1 2,4-D×1.5 or 2.0 mg l-1 showed the highest fresh weight (FW) and the lowest time required to start callus induction, respectively. As for the production of SC, the B5 media with 2.0 mg l-1 of Ag-NPs achieved the highest concentration of alkaloid compounds represented by trigonelline and diosgenin, and flavonoids expressed by qurcetine, vitxein, rutin, apigenin, isovitexin, and kaemferol.
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Claims 7
Claim · model-written summary
LS with 3.0 mg l-1 2,4-D and 1.0 mg L-1 BA gave the highest callus induction.
- Taxon
- Trigonella foenum-graecum L.
Trigonella foenum-graecumt0 - Treatment
- culture media LS with 3.0 mg l-1 of 2,4-D and 1.0 mg L-1 of BAe0
- Outcome
- percentage of callus inductione0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“culture media LS with 3.0 mg l-1 of 2,4-D and 1.0 mg L-1 of BA the highest percentage of callus induction”
t0“Trigonella foenum-graecum L.”
Claim · model-written summary
The same LS combination minimized days to complete callus formation.
- Taxon
- Trigonella foenum-graecum L.Trigonella foenum-graecumt0
- Treatment
- culture media LS with 3.0 mg l-1 of 2,4-D and 1.0 mg L-1 of BAe0
- Outcome
- days to complete callus formatione0
- Direction
- minimume0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“culture media LS with 3.0 mg l-1 of 2,4-D and 1.0 mg L-1 of BA the highest percentage of callus induction and the minimum days to complete callus formation”
t0“Trigonella foenum-graecum L.”
Claim · model-written summary
LS with 2.0 mg l-1 2,4-D and 1.0 mg l-1 BA gave the highest dry weight and relative water content.
- Taxon
- Trigonella foenum-graecum L.Trigonella foenum-graecumt0
- Treatment
- 2.0 mg l-1 2,4-D and 1.0 mg l-1 of BAe0
- Outcome
- dry weight (DW) and relative water content (RWC)e0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“same media with 2.0 mg l-1 2,4-D and 1.0 mg l-1 of BA recorded the highest dry weight (DW) and relative water content (RWC)”
t0“Trigonella foenum-graecum L.”
Claim · model-written summary
B5 with 2.0 mg l-1 2,4-D and 1.5 mg l-1 gave the highest fresh weight.
- Taxon
- Trigonella foenum-graecum L.Trigonella foenum-graecumt0
- Treatment
- B5×2.0 mg l-1 2,4-D×1.5e0
- Outcome
- fresh weight (FW)e0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B5×2.0 mg l-1 2,4-D×1.5 or 2.0 mg l-1 showed the highest fresh weight (FW) and the lowest time required to start callus induction, respectively”
t0“Trigonella foenum-graecum L.”
Claim · model-written summary
B5 with 2.0 mg l-1 2,4-D and 2.0 mg l-1 minimized time to callus induction.
- Taxon
- Trigonella foenum-graecum L.Trigonella foenum-graecumt0
- Treatment
- 2.0 mg l-1e0
- Outcome
- time required to start callus inductione0
- Direction
- loweste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B5×2.0 mg l-1 2,4-D×1.5 or 2.0 mg l-1 showed the highest fresh weight (FW) and the lowest time required to start callus induction, respectively”
t0“Trigonella foenum-graecum L.”
Claim · model-written summary
B5 with 2.0 mg l-1 silver nanoparticles maximized trigonelline and diosgenin concentrations.
- Taxon
- Trigonella foenum-graecum L.Trigonella foenum-graecumt0
- Treatment
- B5 media with 2.0 mg l-1 of Ag-NPse0
- Outcome
- concentration of alkaloid compounds represented by trigonelline and diosgenine0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B5 media with 2.0 mg l-1 of Ag-NPs achieved the highest concentration of alkaloid compounds represented by trigonelline and diosgenin”
t0“Trigonella foenum-graecum L.”
Claim · model-written summary
B5 with 2.0 mg l-1 silver nanoparticles maximized six named flavonoids.
- Taxon
- Trigonella foenum-graecum L.Trigonella foenum-graecumt0
- Treatment
- B5 media with 2.0 mg l-1 of Ag-NPse0
- Outcome
- flavonoids expressed by qurcetine, vitxein, rutin, apigenin, isovitexin, and kaemferole0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“B5 media with 2.0 mg l-1 of Ag-NPs achieved the highest concentration of alkaloid compounds represented by trigonelline and diosgenin, and flavonoids expressed by qurcetine, vitxein, rutin, apigenin, isovitexin, and kaemferol”
t0“Trigonella foenum-graecum L.”
The same questions in other papers
2,4-D · growth · Trigonella foenum-graecum (fenugreek)Results agree
2 papers in the same direction as this paper
- positive
- positive
N-benzyladenine · growth · Trigonella foenum-graecum (fenugreek)Results agree
4 papers in the same direction as this paper
- positive
- positive
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1 paper more in this group
See all 5 papers →in vitro · growth · Trigonella foenum-graecum (fenugreek)Results agree
1 paper in the same direction as this paper
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nanoparticle · quality: phytochemicals · Trigonella foenum-graecum (fenugreek)Results agree
1 paper in the same direction as this paper
- positive
nanoparticle · molecular: expression · Trigonella foenum-graecum (fenugreek)Results agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.