Abstract
The efficiency of regeneration media, based on the morpho-cytochemical response and embryogenesis gene expression of widely cultivated cotton in Indonesia (Gossypium hirsutum L.), needs to be studied further. This study's objectives are to identify the effect of 2,4-D and IBA hormones on the morphology and induction of cotton callus; to determine the effect of regeneration media on the morphology, cytochemical, and gene expression of cotton plant during embryogenesis. This study was conducted by inducing cotton callus on MS0 medium, MS Basal + 0.1 mg/L 2,4-D and MS Basal + 0.1 mg/L IBA. The best callus induction results were regenerated on the regeneration media, namely M1 (MS Vitamin + 0.01 ppm 2,4-D + 0.3 ppm IBA + 0.5 ppm Kinetin), M2 (MS Vitamin + 0.01 ppm 2,4-D + 0.5 ppm IBA + 0.5 ppm Kinetin), M3 (MS Vitamin + 0.00 ppm 2,4-D + 0.3 ppm IBA + 0.5 ppm Kinetin), and M4 (MS Vitamin + 0, 00 ppm 2,4- D + 0.5 ppm IBA + 0.5 ppm Kinetin). The embryogenesis gene expression was observed. The best morphological results for callus induction were obtained on MS Basal + 0.1 mg/L 2,4-D medium with a compact and white callus, indicates high embryogenic and regeneration ability. The highest percentage of regenerated callus parameters was found in M2 media and further confirmed by cytochemical characterization. Embryogenesis genes expressed were GhSERK1, GhSERK2, LEC, GhWUS, and GhWOX11. Other fiber-related genes GhMYB25-like, GhHD-1, and F3H were also expressed during the embryogenesis.
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Claims 5
Claim · model-written summary
MS Basal with 0.1 mg/L 2,4-D gave the best callus induction morphology, with compact white callus.
- Taxon
- cotton
Gossypium hirsutume1 - Treatment
- MS Basal + 0.1 mg/L 2,4-D mediume0
- Outcome
- compact and white calluse0
- Qualifier
- high embryogenic and regeneration abilitye0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The best morphological results for callus induction were obtained on MS Basal + 0.1 mg/L 2,4-D medium with a compact and white callus, indicates high embryogenic and regeneration ability.”
e1“induction of cotton callus”
Claim · model-written summary
M2 medium had the highest percentage of regenerated cotton callus, supported by cytochemical characterization.
- Taxon
- cottonGossypium hirsutume1
- Treatment
- M2 mediae0
- Outcome
- percentage of regenerated callus parameterse0
- Direction
- higheste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The highest percentage of regenerated callus parameters was found in M2 media and further confirmed by cytochemical characterization.”
e1“induction of cotton callus”
Claim · model-written summary
Cotton embryogenesis expressed GhSERK1, GhSERK2 and LEC.
- Taxon
- cottonGossypium hirsutume1
- Organ / stage
- embryogenesise1
- Gene
- GhSERK1, GhSERK2, LECe0
- Outcome
- Embryogenesis genes expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryogenesis genes expressed were GhSERK1, GhSERK2, LEC, GhWUS, and GhWOX11.”
e1“cotton plant during embryogenesis”
Claim · model-written summary
Cotton embryogenesis expressed GhWUS and GhWOX11.
- Taxon
- cottonGossypium hirsutume1
- Organ / stage
- embryogenesise1
- Gene
- GhWUS, and GhWOX11e0
- Outcome
- Embryogenesis genes expressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Embryogenesis genes expressed were GhSERK1, GhSERK2, LEC, GhWUS, and GhWOX11.”
e1“cotton plant during embryogenesis”
Claim · model-written summary
The fiber-related genes GhMYB25-like, GhHD-1 and F3H were expressed during cotton embryogenesis.
- Taxon
- cottonGossypium hirsutume1
- Organ / stage
- embryogenesise0
- Gene
- GhMYB25-like, GhHD-1, and F3He0
- Outcome
- were also expressed during the embryogenesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Other fiber-related genes GhMYB25-like, GhHD-1, and F3H were also expressed during the embryogenesis.”
e1“cotton plant during embryogenesis”
The same questions in other papers
2,4-D · growth · Gossypium hirsutum (cotton)Positive and negative results
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- negative
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- negative
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See all 39 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.