Abstract
Exogenous methyl jasmonate (MeJA) application has shown promising effects on plant defense under diverse abiotic stresses. However, the mechanisms underlying MeJA-induced stress resistance in bananas are unclear. Therefore, in this study, we treated banana plants with 100 μM MeJA before inducing osmotic stress using mannitol. Plant phenotype and antioxidant enzyme activity results demonstrated that MeJA improved osmotic stress resistance in banana plants. Thereafter, to explore the molecular mechanisms underlying MeJA-induced osmotic stress resistance in banana seedlings, we conducted high-throughput RNA sequencing (RNA-seq) using leaf and root samples of “Brazilian” banana seedlings treated with MeJA for 0 h and 8 h. RNA-seq analysis showed that MeJA treatment upregulated 1506 (leaf) and 3341 (root) genes and downregulated 1768 (leaf) and 4625 (root) genes. Then, we performed gene ontology and Kyoto Encyclopedia of Genes and Genomes analyses on the differentially expressed genes. We noted that linoleic acid metabolism was enriched in both root and leaf samples, and the genes of this pathway exhibited different expression patterns; 9S-LOX genes were highly induced by MeJA in the leaves, whereas 13S-LOX genes were highly induced in the roots. We also identified the promoters of these genes, as the differences in response elements may contribute to tissue-specific gene expression in response to MeJA application in banana seedlings. Overall, the findings of this study provide insights into the mechanisms underlying abiotic stress resistance in banana that may aid in the improvement of banana varieties relying on molecular breeding.
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Claims 9
Claim · model-written summary
MeJA improved banana osmotic-stress resistance.
- Taxon
- banana plantse0
- Treatment
- 100 μM MeJAe1
- Outcome
- osmotic stress resistancee0
- Direction
- improvede0
- Qualifier
- osmotic stress using mannitole1
Not reportedComparator
Verbatim
e0“MeJA improved osmotic stress resistance in banana plants”
e1“treated banana plants with 100 μM MeJA before inducing osmotic stress using mannitol”
Claim · model-written summary
MeJA upregulated 1506 leaf genes.
Verbatim
e0“MeJA treatment upregulated 1506 (leaf) and 3341 (root) genes”
e1“banana seedlings treated with MeJA for 0 h and 8 h”
Claim · model-written summary
MeJA upregulated 3341 root genes.
Verbatim
e0“MeJA treatment upregulated 1506 (leaf) and 3341 (root) genes”
e1“banana seedlings treated with MeJA for 0 h and 8 h”
Claim · model-written summary
MeJA downregulated 1768 leaf genes.
Verbatim
e0“downregulated 1768 (leaf) and 4625 (root) genes”
e1“MeJA treatment”
e2“banana seedlings treated with MeJA for 0 h and 8 h”
Claim · model-written summary
MeJA downregulated 4625 root genes.
Verbatim
e0“downregulated 1768 (leaf) and 4625 (root) genes”
e1“MeJA treatment”
e2“banana seedlings treated with MeJA for 0 h and 8 h”
Claim · model-written summary
Linoleic acid metabolism was enriched in banana roots and leaves.
Verbatim
e0“linoleic acid metabolism was enriched in both root and leaf samples”
e1““Brazilian” banana seedlings treated with MeJA for 0 h and 8 h”
Claim · model-written summary
MeJA strongly induced 9S-LOX genes in banana leaves.
- Taxon
- banana seedlingse1
- Organ / stage
- leavese0
- Treatment
- MeJAe0
- Gene
- 9S-LOX genese0
- Outcome
- 9S-LOX genese0
- Direction
- highly inducede0
Not reportedComparator
Verbatim
e0“9S-LOX genes were highly induced by MeJA in the leaves”
e1““Brazilian” banana seedlings treated with MeJA for 0 h and 8 h”
Claim · model-written summary
MeJA strongly induced 13S-LOX genes in banana roots.
- Taxon
- banana seedlingse2
- Organ / stage
- rootse0
- Treatment
- MeJAe1
- Gene
- 13S-LOX genese0
- Outcome
- 13S-LOX genese0
- Direction
- highly inducede0
Not reportedComparator
Verbatim
e0“13S-LOX genes were highly induced in the roots”
e1“by MeJA in the leaves, whereas 13S-LOX genes were highly induced in the roots”
e2““Brazilian” banana seedlings treated with MeJA for 0 h and 8 h”
Claim · model-written summary
The authors identified promoters whose response-element differences may contribute to tissue-specific MeJA responses.
Verbatim
e0“We also identified the promoters of these genes, as the differences in response elements may contribute to tissue-specific gene expression in response to MeJA application in banana seedlings”
The same questions in other papers
(-)-methyl jasmonate · tolerance: abiotic · MusaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.