Abstract
Abstract Fungal diseases are crucial factors in reducing chickpea production. Ascochyta Blight is caused by the necrotrophic fungi Didymella rabiei and is one of the most destructive diseases in most world areas. Therefore, a completely randomized factorial design with five replications was applied to evaluate the effects of Ascochyta Blight disease fungi on the chickpea plant. The chlorophyll index, the activities of superoxide dismutase isoforms, and evolutionary analyses were performed to get further insights. Also, 2D electrophoresis of chickpea leaf proteins, gene ontology, and protein-protein interactions analysis was performed. The results did not show any significant effect of A. rabiei infection on the wet weight of chickpea seedlings. Chlorophyll index levels significantly decreased with A. rabiei infection in both chickpea lines. Electrophoretic analysis of superoxide dismutase on 8% polyacrylamide gel revealed three isoforms. The activities of superoxide dismutase isoforms significantly increased with A. rabiei disease. Identification of proteins was performed according to their isoelectric points and approximate molecular weights. Leaf proteome analysis of chickpea lines showed that the expression of eight reproducible spots changed significantly under A. rabiei disease condition. Candidate proteins were components of defense and regulation systems. High expression of Dual specificity protein, Peptide methionine sulfoxide reductase B2, and chloroplastic phosphatase 1B (the proteins involved in the defense system) reveals their essential functions under A. rabiei infection. Increased activities of superoxide dismutase enzymes and proteins involved in the defense system can reduce A. rabiei infection effects on chickpea seedlings.
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Claims 6
Claim · model-written summary
A. rabiei infection did not significantly affect chickpea seedling wet weight.
- Taxon
- chickpea
Cicer arietinume0 - Treatment
- A. rabiei infectione0
- Interactor
- A. rabiei
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Ascochyta rabieie0pathogen - Outcome
- wet weight of chickpea seedlingse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“The results did not show any significant effect of A. rabiei infection on the wet weight of chickpea seedlings”
Claim · model-written summary
A. rabiei infection significantly decreased the chlorophyll index in both lines.
- Taxon
- chickpeaCicer arietinume0
- Treatment
- A. rabiei infectione0
- Interactor
- A. rabieiAscochyta rabieie0pathogen
- Outcome
- Chlorophyll index levelse0
- Direction
- decreasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Chlorophyll index levels significantly decreased with A. rabiei infection in both chickpea lines”
Claim · model-written summary
Three superoxide dismutase isoforms were detected.
- Taxon
- chickpeaCicer arietinumt0
- Gene
- superoxide dismutasee0
- Outcome
- three isoformse0
- Value
- three isoformse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Electrophoretic analysis of superoxide dismutase on 8% polyacrylamide gel revealed three isoforms”
t0“Evaluation of Superoxide Dismutase Isoforms Activity and Defense System-Related Proteins’ Expression in Ascochyta Blight-Infected Chickpea Using 2D Electrophoresis Technique”
Claim · model-written summary
Superoxide dismutase isoform activities significantly increased with A. rabiei disease.
- Taxon
- chickpeaCicer arietinumt0
- Treatment
- A. rabiei diseasee0
- Interactor
- A. rabieiAscochyta rabieie0pathogen
- Gene
- superoxide dismutasee0
- Outcome
- activities of superoxide dismutase isoformse0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The activities of superoxide dismutase isoforms significantly increased with A. rabiei disease”
t0“Evaluation of Superoxide Dismutase Isoforms Activity and Defense System-Related Proteins’ Expression in Ascochyta Blight-Infected Chickpea Using 2D Electrophoresis Technique”
Claim · model-written summary
Eight reproducible leaf proteome spots changed significantly during disease.
- Taxon
- chickpeaCicer arietinume0
- Organ / stage
- Leafe0
- Treatment
- A. rabiei disease conditione0
- Interactor
- A. rabieiAscochyta rabieie0pathogen
- Outcome
- expression of eight reproducible spotse0
- Value
- eight reproducible spotse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Leaf proteome analysis of chickpea lines showed that the expression of eight reproducible spots changed significantly under A. rabiei disease condition”
Claim · model-written summary
Defense proteins had high expression under infection.
- Taxon
- chickpeaCicer arietinumt0
- Interactor
- A. rabieiAscochyta rabieie0pathogen
- Gene
- Dual specificity protein, Peptide methionine sulfoxide reductase B2, and chloroplastic phosphatase 1Be0
- Outcome
- High expressione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“High expression of Dual specificity protein, Peptide methionine sulfoxide reductase B2, and chloroplastic phosphatase 1B (the proteins involved in the defense system) reveals their essential functions under A. rabiei infection”
t0“Evaluation of Superoxide Dismutase Isoforms Activity and Defense System-Related Proteins’ Expression in Ascochyta Blight-Infected Chickpea Using 2D Electrophoresis Technique”
The same questions in other papers
Ascochyta rabiei · physiology: antioxidant enzymes · Cicer arietinum (chickpea)Results agree
1 paper in the same direction as this paper
- positive
infection · physiology: pigments · Cicer arietinum (chickpea)Positive and negative results
1 in the opposite direction
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.