Abstract
Crop roots are colonized by large numbers of microorganisms, collectively known as the root-microbiome, which modulate plant growth, development and contribute to elemental nutrient uptake. In conditions of nitrogen limitation, the over-expressed Calcineurin B-like interacting protein kinase 2 (OsCIPK2) gene with root-specific promoter (RC) has been shown to enhance growth and nitrogen uptake in rice. Analysis of root-associated bacteria through high-throughput sequencing revealed that OsCIPK2 has a significant impact on the diversity of the root microbial community under low nitrogen stress. The quantification of nifH gene expression demonstrated a significant enhancement in nitrogen-fixing capabilities in the roots of RC transgenetic rice. Synthetic microbial communities (SynCom) consisting of six nitrogen-fixing bacterial strains were observed to be enriched in the roots of RC, leading to a substantial improvement in rice growth and nitrogen uptake in nitrogen-deficient soils. Forty and twenty-three metabolites exhibiting differential abundance were identified in the roots and rhizosphere soils of RC transgenic rice compared to wild-type (WT) rice. These findings suggest that OSCIPK2 plays a role in restructuring the microbial community in the roots through the regulation of metabolite synthesis and secretion. Further experiments involving the exogenous addition of citric acid revealed that an optimal concentration of this compound facilitated the growth of nitrogen-fixing bacteria and substantially augmented their population in the soil, highlighting the importance of citric acid in promoting nitrogen fixation under conditions of low nitrogen availability. These findings suggest that OsCIPK2 plays a role in enhancing nitrogen uptake by rice plants from the soil by influencing the assembly of root microbial communities, thereby offering valuable insights for enhancing nitrogen utilization in rice cultivation.
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Claims 11
Claim · model-written summary
OsCIPK2 overexpression enhanced rice growth and nitrogen uptake under nitrogen limitation.
- Taxon
- rice
Oryza sativae0 - Treatment
- over-expressed Calcineurin B-like interacting protein kinase 2 (OsCIPK2) genee0
- Gene
- OsCIPK2e0
- Outcome
- growth and nitrogen uptakee0
- Direction
- enhancee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“over-expressed Calcineurin B-like interacting protein kinase 2 (OsCIPK2) gene with root-specific promoter (RC) has been shown to enhance growth and nitrogen uptake in rice”
Claim · model-written summary
OsCIPK2 significantly affected root microbial diversity under low nitrogen.
- Taxon
- riceOryza sativat0
- Gene
- OsCIPK2e0
- Outcome
- diversity of the root microbial communitye0
- Qualifier
- under low nitrogen stresse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsCIPK2 has a significant impact on the diversity of the root microbial community under low nitrogen stress”
t0“rice”
Claim · model-written summary
Nitrogen fixing capability was enhanced in RC rice roots.
- Taxon
- riceOryza sativae0
- Material
- RC transgenetic ricee0
- Organ / stage
- rootse0
- Outcome
- nitrogen-fixing capabilitiese0
- Direction
- enhancemente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“a significant enhancement in nitrogen-fixing capabilities in the roots of RC transgenetic rice”
Claim · model-written summary
Six nitrogen fixing bacterial strains were enriched in RC rice roots.
- Taxon
- riceOryza sativat0
- Material
- RCe0
- Interactor
- nitrogen-fixing bacterial strainse0symbiont
- Outcome
- enriched in the rootse0
- Value
- sixe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Synthetic microbial communities (SynCom) consisting of six nitrogen-fixing bacterial strains were observed to be enriched in the roots of RC”
t0“rice”
Claim · model-written summary
The enriched SynCom improved rice growth in nitrogen deficient soil.
- Taxon
- riceOryza sativae0
- Treatment
- Synthetic microbial communities (SynCom)e1
- Outcome
- rice growthe0
- Direction
- improvemente0
- Setting
- nitrogen-deficient soilse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“leading to a substantial improvement in rice growth and nitrogen uptake in nitrogen-deficient soils”
t0“OsCIPK2 mediated rice root microorganisms and metabolites to improve plant nitrogen uptake”
e1“Synthetic microbial communities (SynCom) consisting of six nitrogen-fixing bacterial strains were observed to be enriched in the roots of RC”
Claim · model-written summary
The enriched SynCom improved rice nitrogen uptake in nitrogen deficient soil.
- Taxon
- riceOryza sativae0
- Treatment
- Synthetic microbial communities (SynCom)e1
- Outcome
- nitrogen uptakee0
- Direction
- improvemente0
- Setting
- nitrogen-deficient soilse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“leading to a substantial improvement in rice growth and nitrogen uptake in nitrogen-deficient soils”
t0“OsCIPK2 mediated rice root microorganisms and metabolites to improve plant nitrogen uptake”
e1“Synthetic microbial communities (SynCom) consisting of six nitrogen-fixing bacterial strains were observed to be enriched in the roots of RC”
Claim · model-written summary
Forty root metabolites differed between RC and wild type rice.
- Taxon
- riceOryza sativae0
- Material
- RC transgenic ricee0
- Organ / stage
- rootse0
- Outcome
- metabolites exhibiting differential abundancee0
- Value
- Fortye0
- Comparator
- wild-type (WT) ricee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Forty and twenty-three metabolites exhibiting differential abundance were identified in the roots and rhizosphere soils of RC transgenic rice compared to wild-type (WT) rice”
Claim · model-written summary
Twenty-three rhizosphere soil metabolites differed between RC and wild type rice.
- Taxon
- riceOryza sativae0
- Material
- RC transgenic ricee0
- Outcome
- metabolites exhibiting differential abundancee0
- Value
- twenty-threee0
- Comparator
- wild-type (WT) ricee0
- Setting
- rhizosphere soilse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Forty and twenty-three metabolites exhibiting differential abundance were identified in the roots and rhizosphere soils of RC transgenic rice compared to wild-type (WT) rice”
Claim · model-written summary
Optimal citric acid promoted nitrogen fixing bacterial growth.
- Taxon
- riceOryza sativat0
- Treatment
- citric acide1
- Interactor
- nitrogen-fixing bacteriae0symbiont
- Outcome
- growth of nitrogen-fixing bacteriae0
- Direction
- facilitatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“an optimal concentration of this compound facilitated the growth of nitrogen-fixing bacteria”
t0“rice”
e1“Further experiments involving the exogenous addition of citric acid revealed that an optimal concentration of this compound facilitated the growth of nitrogen-fixing bacteria”
Claim · model-written summary
Citric acid augmented the nitrogen fixing bacterial population in soil.
- Taxon
- riceOryza sativat0
- Treatment
- citric acide1
- Outcome
- population in the soile0
- Direction
- augmentede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“substantially augmented their population in the soil”
t0“rice”
e1“Further experiments involving the exogenous addition of citric acid revealed that an optimal concentration of this compound facilitated the growth of nitrogen-fixing bacteria”
Claim · model-written summary
The authors propose OsCIPK2 influences microbial assembly through metabolite synthesis and secretion.
- Taxon
- riceOryza sativat0
- Gene
- OSCIPK2e0
- Outcome
- restructuring the microbial community in the rootse0
- Qualifier
- regulation of metabolite synthesis and secretione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OSCIPK2 plays a role in restructuring the microbial community in the roots through the regulation of metabolite synthesis and secretion”
t0“rice”
The same questions in other papers
citric acid · growth · Oryza sativa (rice)Positive and negative results
4 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- negative
- positive
3 papers more in this group
See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.