Abstract
DNA methylation is one of induced changes under salinity stress causing reduction in the expression of several crucial genes required for normal plant's operation. Potential use of royal jelly (RJ), folic acid (FA) and 5-azacitidine (5-AZA) on two Egyptian faba bean varieties (Sakha-3 and Giza-716) grown under saline conditions was investigated. Salinity stress affects negatively on seeds germination (G %), mitotic index, membrane stability and induced a significant increase in chromosomal abnormalities (CAs). DNA methyltransferases genes ( MT1 and MT2 ) were highly up-regulated (∼23 and 8 folds for MT1 and MT2 in shoots of Giza-716 stressed plants). On the other hand, down regulation of other studied stress related genes: superoxide dismutase ( SOD ), catalase (CAT ), glutathione reductase ( GR ), heat shock protein ( HSP-17.9 ) and proline-rich protein ( GPRP ) were detected in stressed plants of both studied varieties. Treating plants with RJ and FA increase G%, chlorophyll content, improves membrane properties and reduces CAs compared to non-treated stressed plants. Exogenous application of 5-AZA, RJ and FA on salinity stressed plants was associated with a significant reduction in the transcription of MT1 and MT2 which was associated with significant up regulation in the expression of Cu/Zn-SOD , CAT , GR , GPRP and HSP-17.9 encoding genes. The Lowest expression of MT1 and MT2 were induced with 5-AZA treatment in both studied varieties. Exogenous application of the FA, RJ and 5-AZA modified the methylation state of stressed plants by regulation the expression of DNA methyltransferases, subsequently, modulated the expression of studied genes and could be proposed as a promising treatment to ameliorate hazardous effects of salt stress on different plants.
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Claims 16
Claim · model-written summary
Salinity reduced seed germination, mitotic index and membrane stability.
- Taxon
- Vicia faba L.
Vicia fabat0 - Treatment
- Salinity stresse0
- Outcome
- seeds germination (G %), mitotic index, membrane stabilitye0
- Direction
- negativelye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salinity stress affects negatively on seeds germination (G %), mitotic index, membrane stability”
t0“Vicia faba L. plants”
Claim · model-written summary
Salinity significantly increased chromosomal abnormalities.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- Salinity stresse0
- Outcome
- chromosomal abnormalities (CAs)e0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Salinity stress affects negatively on seeds germination (G %), mitotic index, membrane stability and induced a significant increase in chromosomal abnormalities (CAs)”
t0“Vicia faba L. plants”
Claim · model-written summary
MT1 was upregulated about 23 fold in stressed Giza-716 shoots.
- Taxon
- Vicia faba L.Vicia fabat0
- Material
- Giza-716e0
- Organ / stage
- shootse0
- Gene
- MT1e0
- Outcome
- MT1e0
- Direction
- up-regulatede0
- Value
- ∼23e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA methyltransferases genes ( MT1 and MT2 ) were highly up-regulated (∼23 and 8 folds for MT1 and MT2 in shoots of Giza-716 stressed plants)”
t0“Vicia faba L. plants”
Claim · model-written summary
MT2 was upregulated about eight fold in stressed Giza-716 shoots.
- Taxon
- Vicia faba L.Vicia fabat0
- Material
- Giza-716e0
- Organ / stage
- shootse0
- Gene
- MT2e0
- Outcome
- MT2e0
- Direction
- up-regulatede0
- Value
- 8 foldse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“DNA methyltransferases genes ( MT1 and MT2 ) were highly up-regulated (∼23 and 8 folds for MT1 and MT2 in shoots of Giza-716 stressed plants)”
t0“Vicia faba L. plants”
Claim · model-written summary
Stress related SOD, CAT and GR were downregulated in both varieties.
- Taxon
- Vicia faba L.Vicia fabat0
- Gene
- SOD ), catalase (CAT ), glutathione reductase ( GRe0
- Outcome
- stress related genese0
- Direction
- down regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“down regulation of other studied stress related genes: superoxide dismutase ( SOD ), catalase (CAT ), glutathione reductase ( GR ), heat shock protein ( HSP-17.9 ) and proline-rich protein ( GPRP ) were detected in stressed plants of both studied varieties”
t0“Vicia faba L. plants”
Claim · model-written summary
Stress related HSP-17.9 and GPRP were downregulated in both varieties.
- Taxon
- Vicia faba L.Vicia fabat0
- Gene
- HSP-17.9 ) and proline-rich protein ( GPRPe0
- Outcome
- stress related genese0
- Direction
- down regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“down regulation of other studied stress related genes: superoxide dismutase ( SOD ), catalase (CAT ), glutathione reductase ( GR ), heat shock protein ( HSP-17.9 ) and proline-rich protein ( GPRP ) were detected in stressed plants of both studied varieties”
t0“Vicia faba L. plants”
Claim · model-written summary
Royal jelly increased germination and chlorophyll and improved membrane properties under salinity.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- RJe0
- Outcome
- G%, chlorophyll content, improves membrane propertiese0
- Direction
- increasee0
- Comparator
- non-treated stressed plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treating plants with RJ and FA increase G%, chlorophyll content, improves membrane properties and reduces CAs compared to non-treated stressed plants”
t0“Vicia faba L. plants”
Claim · model-written summary
Folic acid increased germination and chlorophyll and improved membrane properties under salinity.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- FAe0
- Outcome
- G%, chlorophyll content, improves membrane propertiese0
- Direction
- increasee0
- Comparator
- non-treated stressed plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treating plants with RJ and FA increase G%, chlorophyll content, improves membrane properties and reduces CAs compared to non-treated stressed plants”
t0“Vicia faba L. plants”
Claim · model-written summary
Royal jelly reduced chromosomal abnormalities under salinity.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- RJe0
- Outcome
- CAse0
- Direction
- reducese0
- Comparator
- non-treated stressed plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treating plants with RJ and FA increase G%, chlorophyll content, improves membrane properties and reduces CAs compared to non-treated stressed plants”
t0“Vicia faba L. plants”
Claim · model-written summary
Folic acid reduced chromosomal abnormalities under salinity.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- FAe0
- Outcome
- CAse0
- Direction
- reducese0
- Comparator
- non-treated stressed plantse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treating plants with RJ and FA increase G%, chlorophyll content, improves membrane properties and reduces CAs compared to non-treated stressed plants”
t0“Vicia faba L. plants”
Claim · model-written summary
5-AZA significantly reduced MT1 and MT2 transcription in salt-stressed plants.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- 5-AZAe0
- Gene
- MT1 and MT2e0
- Outcome
- transcription of MT1 and MT2e0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous application of 5-AZA, RJ and FA on salinity stressed plants was associated with a significant reduction in the transcription of MT1 and MT2”
t0“Vicia faba L. plants”
Claim · model-written summary
RJ significantly reduced MT1 and MT2 transcription in salt-stressed plants.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- RJe0
- Gene
- MT1 and MT2e0
- Outcome
- transcription of MT1 and MT2e0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous application of 5-AZA, RJ and FA on salinity stressed plants was associated with a significant reduction in the transcription of MT1 and MT2”
t0“Vicia faba L. plants”
Claim · model-written summary
FA significantly reduced MT1 and MT2 transcription in salt-stressed plants.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- FAe0
- Gene
- MT1 and MT2e0
- Outcome
- transcription of MT1 and MT2e0
- Direction
- reductione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Exogenous application of 5-AZA, RJ and FA on salinity stressed plants was associated with a significant reduction in the transcription of MT1 and MT2”
t0“Vicia faba L. plants”
Claim · model-written summary
The three applications were associated with upregulation of Cu/Zn-SOD, CAT and GR.
- Taxon
- Vicia faba L.Vicia fabat0
- Gene
- Cu/Zn-SOD , CAT , GRe0
- Outcome
- expressione0
- Direction
- up regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant up regulation in the expression of Cu/Zn-SOD , CAT , GR , GPRP and HSP-17.9 encoding genes”
t0“Vicia faba L. plants”
Claim · model-written summary
The three applications were associated with upregulation of GPRP and HSP-17.9.
- Taxon
- Vicia faba L.Vicia fabat0
- Gene
- GPRP and HSP-17.9e0
- Outcome
- expressione0
- Direction
- up regulatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant up regulation in the expression of Cu/Zn-SOD , CAT , GR , GPRP and HSP-17.9 encoding genes”
t0“Vicia faba L. plants”
Claim · model-written summary
5-AZA produced the lowest MT1 and MT2 expression in both varieties.
- Taxon
- Vicia faba L.Vicia fabat0
- Treatment
- 5-AZA treatmente0
- Gene
- MT1 and MT2e0
- Outcome
- expression of MT1 and MT2e0
- Direction
- Loweste0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The Lowest expression of MT1 and MT2 were induced with 5-AZA treatment in both studied varieties”
t0“Vicia faba L. plants”
The same questions in other papers
folic acid · physiology: pigments · Vicia faba (faba bean)Results agree
1 paper in the same direction as this paper
- positive
salinity · cytology · Vicia faba (faba bean)Results agree
2 papers in the same direction as this paper
- negative
- negative
salinity · physiology: oxidative · Vicia faba (faba bean)Results agree
10 papers in the same direction as this paper
- negative
- negative
- negative
7 papers more in this group
See all 11 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.