Abstract
Rice is the staple food for more than half of the global population. It is cultivated in over 100 countries, and is predominant in Asia, which accounts for 90% of the world's production. As the most crucial food crop globally, rice is sensitive to both UVB radiation and climate variations. UltraViolet-B radiation (UVB) is recognised as adverse factors for photosynthesis and plant growth. The current study investigates the resilience of Oryza sativa L. (IR20) seedlings to UVB irradiation, focusing on parameters such as photosynthetic pigment, total sugar, phenolic, total free amino acids, malondialdehyde content, hydrogen peroxide, and antioxidative enzymes. The mRNA expression levels of genes involved in UVB perception, signalling, and response (UVR8, HY5, RLCK, PHT2, and URP) were studied using quantitative RT-PCR (RT-qPCR). Additionally, a computational approach was employed to identify UVB-responsive microRNAs (miRNAs) and their corresponding target mRNAs; which were subsequently validated using stem-loop RT-qPCR and RT-qPCR, respectively. Surprisingly, it was discovered that the H2O2 content in the IR20 cultivar increased in response to UVB irradiation, despite no significant changes observed in lipid peroxidation, chlorophyll, and phenolic content. Additionally, UVB irradiation increases the total sugar content and decreases the entire free amino acid content, while not affecting total protein. The RT-qPCR analysis of mRNA expression reveals that UVB exposure augment the expression of genes related to UVB signal detection (UVR8), signalling (HY5, RLCK, and WRKY), and response (PHT2, CA, and URP). Our findings also suggest that at the post-transcriptional stage, miRNAs expressed upon UVB irradiation are responsible for the significant tolerance of IR20 seedlings to UVB radiation. Overall, the results reveal that IR20 exhibits promise candidate for the development of rice varieties that are both highly productive and resilient to UVB induced stress.
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Claims 10
Claim · model-written summary
UVB increased H2O2 content in IR20.
- Taxon
- Oryza sativa L.
Oryza sativat0 - Material
- IR20e0
- Treatment
- UVB irradiatione0
- Outcome
- H2O2 contente0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L.”
e0“H2O2 content in the IR20 cultivar increased in response to UVB irradiation”
Claim · model-written summary
UVB caused no significant change in lipid peroxidation, chlorophyll or phenolic content.
- Taxon
- Oryza sativa L.Oryza sativat0
- Treatment
- UVB irradiatione1
- Outcome
- lipid peroxidation, chlorophyll, and phenolic contente0
- Direction
- no significant changese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L.”
e0“no significant changes observed in lipid peroxidation, chlorophyll, and phenolic content”
e1“in response to UVB irradiation”
Claim · model-written summary
UVB increased total sugar content.
- Taxon
- Oryza sativa L.Oryza sativat0
- Treatment
- UVB irradiatione0
- Outcome
- total sugar contente0
- Direction
- increasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L.”
e0“UVB irradiation increases the total sugar content”
Claim · model-written summary
UVB decreased total free amino acid content.
- Taxon
- Oryza sativa L.Oryza sativat0
- Treatment
- UVB irradiatione0
- Outcome
- entire free amino acid contente0
- Direction
- decreasese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L.”
e0“UVB irradiation increases the total sugar content and decreases the entire free amino acid content”
Claim · model-written summary
UVB did not affect total protein.
- Taxon
- Oryza sativa L.Oryza sativat0
- Treatment
- UVB irradiatione1
- Outcome
- total proteine0
- Direction
- not affectinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L.”
e0“while not affecting total protein”
e1“UVB irradiation increases the total sugar content”
Claim · model-written summary
UVB exposure increased UVR8 expression.
- Taxon
- Oryza sativa L.Oryza sativat0
- Treatment
- UVB exposuree0
- Gene
- UVR8e0
- Outcome
- expression of genes related to UVB signal detectione0
- Direction
- augmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L.”
e0“UVB exposure augment the expression of genes related to UVB signal detection (UVR8)”
Claim · model-written summary
UVB exposure increased expression of HY5, RLCK and WRKY signaling genes.
- Taxon
- Oryza sativa L.Oryza sativat0
- Treatment
- UVB exposuree0
- Gene
- HY5, RLCK, and WRKYe0
- Outcome
- expression of genese0
- Direction
- augmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L.”
e0“UVB exposure augment the expression of genes related to UVB signal detection (UVR8), signalling (HY5, RLCK, and WRKY)”
Claim · model-written summary
UVB exposure increased expression of PHT2, CA and URP response genes.
- Taxon
- Oryza sativa L.Oryza sativat0
- Treatment
- UVB exposuree0
- Gene
- PHT2, CA, and URPe0
- Outcome
- expression of genese0
- Direction
- augmente0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L.”
e0“UVB exposure augment the expression of genes related to UVB signal detection (UVR8), signalling (HY5, RLCK, and WRKY), and response (PHT2, CA, and URP)”
Claim · model-written summary
The authors suggest that UVB-expressed miRNAs contribute to significant UVB tolerance in IR20 seedlings.
- Taxon
- Oryza sativa L.Oryza sativat0
- Material
- IR20e0
- Gene
- miRNAse0
- Outcome
- significant tolerance of IR20 seedlings to UVB radiatione0
- Qualifier
- expressed upon UVB irradiatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L.”
e0“miRNAs expressed upon UVB irradiation are responsible for the significant tolerance of IR20 seedlings to UVB radiation”
Claim · model-written summary
The authors identify IR20 as a promising candidate for productive, UVB-resilient rice varieties.
- Taxon
- Oryza sativa L.Oryza sativat0
- Material
- IR20e0
- Outcome
- candidate for the development of rice varieties that are both highly productive and resilient to UVB induced stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Oryza sativa L.”
e0“IR20 exhibits promise candidate for the development of rice varieties that are both highly productive and resilient to UVB induced stress”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.