Abstract
The experiment was carried out at the National Commission for Biotechnology during 2019–2021 with the aim of studying the influence of some abiotic stresses on the fresh and dry weight and the content of vincristine and vinblastine in Catharanthus roseus callus. After sterilization, seeds were cultured in MS medium. Plants were transferred to propagation media. Callus was developed from the leaves using 5C01 medium. After 60 days, the callus was moved to the 5C01 medium with NaCl (0, 30, 60, 90, 120 mM) and PEG-6000 (0, -0.2, -0.4, -0.6 MPa) with a higher level every 30 days. The findings revealed that fresh and dry callus weight considerably decreased in the 120 mM NaCl treatment (3.03, 0.24 g respectively) and at the osmotic stress level of -0.6 Mpa (2.34, 0.19 g respectively). The vincristine and vinblastine content were significantly higher at the NaCl induction level of 90 mM NaCl (94.29, 103.6 μg.g-1 DW respectively), while they were considerably lower at the osmotic stress level of -0.6 Mpa (2.61, 3.12 μg.g-1 DW respectively). This research suggests a new medium (5C01) for callus induction which is distinguished by superior levels of vincristine and vinblastine in the callus compared to other studies.
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Claims 9
Claim · model-written summary
At 120 mM NaCl, fresh callus weight decreased to 3.03 g.
- Taxon
- Catharanthus roseus
Catharanthus roseust0 - Organ / stage
- calluse0
- Treatment
- 120 mM NaCle0
- Outcome
- fresh and dry callus weighte0
- Direction
- decreasede0
- Value
- 3.03e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fresh and dry callus weight considerably decreased in the 120 mM NaCl treatment (3.03, 0.24 g respectively)”
t0“Catharanthus roseus callus”
Claim · model-written summary
At 120 mM NaCl, dry callus weight decreased to 0.24 g.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Organ / stage
- calluse0
- Treatment
- 120 mM NaCle0
- Outcome
- dry callus weighte0
- Direction
- decreasede0
- Value
- 0.24 ge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“fresh and dry callus weight considerably decreased in the 120 mM NaCl treatment (3.03, 0.24 g respectively)”
t0“Catharanthus roseus callus”
Claim · model-written summary
At −0.6 MPa osmotic stress, fresh callus weight decreased to 2.34 g.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Organ / stage
- callust0
- Treatment
- osmotic stress level of -0.6 Mpae0
- Outcome
- fresh and dry callus weighte1
- Direction
- decreasede1
- Value
- 2.34e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at the osmotic stress level of -0.6 Mpa (2.34, 0.19 g respectively)”
e1“fresh and dry callus weight considerably decreased”
t0“Catharanthus roseus callus”
Claim · model-written summary
At −0.6 MPa osmotic stress, dry callus weight decreased to 0.19 g.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Organ / stage
- callust0
- Treatment
- osmotic stress level of -0.6 Mpae0
- Outcome
- fresh and dry callus weighte1
- Direction
- decreasede1
- Value
- 0.19 ge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at the osmotic stress level of -0.6 Mpa (2.34, 0.19 g respectively)”
e1“fresh and dry callus weight considerably decreased”
t0“Catharanthus roseus callus”
Claim · model-written summary
At 90 mM NaCl, vincristine content was significantly higher at 94.29 μg.g-1 DW.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Treatment
- 90 mM NaCle0
- Outcome
- vincristinee0
- Direction
- highere0
- Value
- 94.29e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The vincristine and vinblastine content were significantly higher at the NaCl induction level of 90 mM NaCl (94.29, 103.6 μg.g-1 DW respectively)”
t0“Catharanthus roseus callus”
Claim · model-written summary
At 90 mM NaCl, vinblastine content was significantly higher at 103.6 μg.g-1 DW.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Treatment
- 90 mM NaCle0
- Outcome
- vinblastinee0
- Direction
- highere0
- Value
- 103.6 μg.g-1 DWe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The vincristine and vinblastine content were significantly higher at the NaCl induction level of 90 mM NaCl (94.29, 103.6 μg.g-1 DW respectively)”
t0“Catharanthus roseus callus”
Claim · model-written summary
At −0.6 MPa osmotic stress, vincristine content was lower at 2.61 μg.g-1 DW.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Treatment
- osmotic stress level of -0.6 Mpae0
- Outcome
- vincristinee1
- Direction
- lowere0
- Value
- 2.61e0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they were considerably lower at the osmotic stress level of -0.6 Mpa (2.61, 3.12 μg.g-1 DW respectively)”
e1“The vincristine and vinblastine content”
t0“Catharanthus roseus callus”
Claim · model-written summary
At −0.6 MPa osmotic stress, vinblastine content was lower at 3.12 μg.g-1 DW.
- Taxon
- Catharanthus roseusCatharanthus roseust0
- Treatment
- osmotic stress level of -0.6 Mpae0
- Outcome
- vinblastinee1
- Direction
- lowere0
- Value
- 3.12 μg.g-1 DWe0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“they were considerably lower at the osmotic stress level of -0.6 Mpa (2.61, 3.12 μg.g-1 DW respectively)”
e1“The vincristine and vinblastine content”
t0“Catharanthus roseus callus”
Claim · model-written summary
The authors propose 5C01 medium for callus induction because it produced superior vincristine and vinblastine levels relative to other studies.
Verbatim
e0“This research suggests a new medium (5C01) for callus induction which is distinguished by superior levels of vincristine and vinblastine in the callus compared to other studies.”
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See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.