Abstract
Maintenance of ion homeostasis in plant cells is an essential physiological requirement for sustainable growth, development, and yield of crops. Plants respond to high levels of heavy metals such as copper (Cu) and cadmium (Cd) to avoid irreversible damage at the structural, physiological and molecular levels. Our previous study found that rice germin-like proteins (OsGLPs) are a type of Cu-responsive proteins. The deletion of 10 tandem OsGLP genes on chromosome 8 led to more severe heavy metal toxicity in rice. In this study, we show that rice WRKY transcription factor OsWRKY72 negatively regulates OsGLP8-7 transcription. Overexpression of OsWRKY72 weakens the Cu/Cd tolerance of rice when exposed to Cu and Cd. OsWRKY72 suppressed expression of OsGLP8-7 and lignin synthesis genes, resulting in reduced lignin polymerization and consequently lower lignin accumulation in cell walls, thereby increasing the Cu and Cd accumulation. In addition, OsWRKY53 bound to OsWRKY72 to alleviate the transcriptional inhibition of OsGLP8-7 . These results revealed that OsWRKY72-OsGLP8-7 is an important module response of rice to heavy metal stress, and that transcription factor OsWRKY72 acts upstream of OsGLP8-7 to regulate Cu/Cd toxicity.
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Claims 7
Claim · model-written summary
OsWRKY72 negatively regulates OsGLP8-7 transcription.
- Taxon
- rice
Oryza sativae0 - Gene
- OsWRKY72e0
- Outcome
- OsGLP8-7 transcriptione0
- Direction
- negatively regulatese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“rice WRKY transcription factor OsWRKY72 negatively regulates OsGLP8-7 transcription”
Claim · model-written summary
OsWRKY72 overexpression weakens rice Cu/Cd tolerance.
- Taxon
- riceOryza sativae0
- Treatment
- Overexpression of OsWRKY72e0
- Gene
- OsWRKY72e0
- Outcome
- Cu/Cd tolerancee0
- Direction
- weakense0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of OsWRKY72 weakens the Cu/Cd tolerance of rice when exposed to Cu and Cd.”
Claim · model-written summary
OsWRKY72 suppressed OsGLP8-7 expression.
- Taxon
- riceOryza sativae1
- Gene
- OsWRKY72e0
- Outcome
- expression of OsGLP8-7e0
- Direction
- suppressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsWRKY72 suppressed expression of OsGLP8-7 and lignin synthesis genes”
e1“rice WRKY transcription factor OsWRKY72”
Claim · model-written summary
OsWRKY72 suppressed lignin synthesis gene expression.
- Taxon
- riceOryza sativae1
- Gene
- OsWRKY72e0
- Outcome
- lignin synthesis genese0
- Direction
- suppressede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsWRKY72 suppressed expression of OsGLP8-7 and lignin synthesis genes”
e1“rice WRKY transcription factor OsWRKY72”
Claim · model-written summary
OsWRKY72 reduced lignin polymerization and wall lignin accumulation.
- Taxon
- riceOryza sativae1
- Gene
- OsWRKY72e0
- Outcome
- lignin polymerization and consequently lower lignin accumulation in cell wallse0
- Direction
- reducede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsWRKY72 suppressed expression of OsGLP8-7 and lignin synthesis genes, resulting in reduced lignin polymerization and consequently lower lignin accumulation in cell walls”
e1“rice WRKY transcription factor OsWRKY72”
Claim · model-written summary
Suppression of lignin synthesis increased Cu and Cd accumulation.
- Taxon
- riceOryza sativae1
- Outcome
- Cu and Cd accumulatione0
- Direction
- increasinge0
- Qualifier
- lignin synthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsWRKY72 suppressed expression of OsGLP8-7 and lignin synthesis genes, resulting in reduced lignin polymerization and consequently lower lignin accumulation in cell walls, thereby increasing the Cu and Cd accumulation”
e1“rice WRKY transcription factor OsWRKY72”
Claim · model-written summary
OsWRKY53 bound OsWRKY72 and alleviated inhibition of OsGLP8-7.
- Taxon
- riceOryza sativae1
- Gene
- OsWRKY53e0
- Outcome
- transcriptional inhibition of OsGLP8-7e0
- Direction
- alleviatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsWRKY53 bound to OsWRKY72 to alleviate the transcriptional inhibition of OsGLP8-7”
e1“rice WRKY transcription factor OsWRKY72”