Abstract
Under a water deficit, the protective proteins known as dehydrins (DHNs) prevent nonspecific interactions in protein and membrane structures and their damage, in addition to playing an antioxidant role. The DHNs of a widespread xerophytic species Scots pine (Pinus sylvestris L.) have been poorly studied, and their role in resistance to water deficits has not been revealed. In this paper, we have expanded the list of DHNs that accumulate in the cells of Scots pine under the conditions of water deficits and revealed their relationship with the effects of water deficits. In this investigation, callus cultures of branches and buds of Scots pine were used. A weak water deficit was created by adding polyethylene glycol to the culture medium. Under the conditions of a water deficit, the activity of catalase and peroxidase enzymes increased in the callus cultures. A moderate decrease in the total water content was correlated with a decrease in the growth rate of the callus cultures, as well as with an increase in the activity of lipid peroxidation. The accumulation of Mr 72, 38, and 27 kDa DHNs occurred in the callus cultures of buds, and the accumulation of Mr 72 and 27 kDa DHNs positively correlated with the lipid peroxidation activity. An increase in the content of DHNs was observed in cultures that differed in origin, growth indicators, and biochemical parameters, indicating the universality of this reaction. Thus, previously undescribed DHNs were identified, the accumulation of which is caused by water deficiency and is associated with manifestations of oxidative stress in the kidney cells of Scots pine.
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Claims 8
Claim · model-written summary
Water deficit increased catalase and peroxidase enzyme activity in Scots pine callus cultures.
- Taxon
- Scots pine
Pinus sylvestrise0 - Material
- callus culturese2
- Treatment
- water deficite2
- Gene
- catalase and peroxidasee2
- Outcome
- activity of catalase and peroxidase enzymese2
- Direction
- increasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The DHNs of a widespread xerophytic species Scots pine (Pinus sylvestris L.) have been poorly studied”
e2“Under the conditions of a water deficit, the activity of catalase and peroxidase enzymes increased in the callus cultures”
Claim · model-written summary
A moderate decrease in total water content was associated with a decrease in growth rate of the callus cultures.
- Taxon
- Scots pinePinus sylvestrise0
- Material
- callus culturese3
- Treatment
- moderate decrease in the total water contente3
- Outcome
- growth rate of the callus culturese3
- Direction
- decreasee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The DHNs of a widespread xerophytic species Scots pine (Pinus sylvestris L.) have been poorly studied”
e3“A moderate decrease in the total water content was correlated with a decrease in the growth rate of the callus cultures, as well as with an increase in the activity of lipid peroxidation”
Claim · model-written summary
A moderate decrease in total water content was associated with an increase in lipid peroxidation activity.
- Taxon
- Scots pinePinus sylvestrise0
- Treatment
- moderate decrease in the total water contente3
- Outcome
- activity of lipid peroxidatione3
- Direction
- increasee3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The DHNs of a widespread xerophytic species Scots pine (Pinus sylvestris L.) have been poorly studied”
e3“A moderate decrease in the total water content was correlated with a decrease in the growth rate of the callus cultures, as well as with an increase in the activity of lipid peroxidation”
Claim · model-written summary
Mr 72, 38 and 27 kDa dehydrins accumulated in callus cultures of buds.
- Taxon
- Scots pinePinus sylvestrise0
- Material
- callus culturese4
- Organ / stage
- budse4
- Gene
- DHNse4
- Outcome
- accumulation of Mr 72, 38, and 27 kDa DHNse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The DHNs of a widespread xerophytic species Scots pine (Pinus sylvestris L.) have been poorly studied”
e4“The accumulation of Mr 72, 38, and 27 kDa DHNs occurred in the callus cultures of buds, and the accumulation of Mr 72 and 27 kDa DHNs positively correlated with the lipid peroxidation activity”
Claim · model-written summary
Accumulation of Mr 72 and 27 kDa dehydrins was positively correlated with lipid peroxidation activity.
- Taxon
- Scots pinePinus sylvestrise0
- Gene
- DHNse4
- Outcome
- accumulation of Mr 72 and 27 kDa DHNse4
- Direction
- positively correlatede4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The DHNs of a widespread xerophytic species Scots pine (Pinus sylvestris L.) have been poorly studied”
e4“The accumulation of Mr 72, 38, and 27 kDa DHNs occurred in the callus cultures of buds, and the accumulation of Mr 72 and 27 kDa DHNs positively correlated with the lipid peroxidation activity”
Claim · model-written summary
The content of dehydrins increased in cultures that differed in origin, growth indicators and biochemical parameters.
- Taxon
- Scots pinePinus sylvestrise0
- Gene
- DHNse5
- Outcome
- content of DHNse5
- Direction
- increasee5
- Qualifier
- universality of this reactione5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The DHNs of a widespread xerophytic species Scots pine (Pinus sylvestris L.) have been poorly studied”
e5“An increase in the content of DHNs was observed in cultures that differed in origin, growth indicators, and biochemical parameters, indicating the universality of this reaction”
Claim · model-written summary
Previously undescribed dehydrins were identified in Scots pine callus cultures.
- Taxon
- Scots pinePinus sylvestrise6
- Gene
- DHNse6
- Outcome
- previously undescribed DHNs were identifiede6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Thus, previously undescribed DHNs were identified, the accumulation of which is caused by water deficiency and is associated with manifestations of oxidative stress in the kidney cells of Scots pine”
Claim · model-written summary
Dehydrin accumulation is caused by water deficiency and is associated with oxidative stress in Scots pine cells.
- Taxon
- Scots pinePinus sylvestrise6
- Treatment
- water deficiencye6
- Gene
- DHNse6
- Outcome
- accumulatione6
- Direction
- increaseinferrede6
- Qualifier
- associated with manifestations of oxidative stresse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e6“Thus, previously undescribed DHNs were identified, the accumulation of which is caused by water deficiency and is associated with manifestations of oxidative stress in the kidney cells of Scots pine”