Abstract
Aim. The response of hemp (Cannabis sativa L.) to artificially simulated salt stress in vitro culture was the aim of our research. Methods. The seeds of the Hlyana variety were cultivated under conditions of salt stress, which was artificially simulated by adding a certain salt to the Murashige & Skoog nutrient medium in accordance with the main types of soil salinity: chloride, sulfate, and carbonate salinity. Results. Hemp clearly responded to an increase in the concentration of salts in the nutrient medium, in particular, a decrease in seed germination was observed. The most suitable for cultivation is the nutrient medium with sulfates, and the least – with carbonates (at relatively low concentrations). Conclusions. Test systems (including the concentrations of the studied salts) were developed and tested for salt stress screening. The test systems were selective in their selection. Selective concentrations of salts in the environment are determined as follows: chlorides – 0.25 NaCl, 0.75 % MgCl2 · 6 H2O; sulfates – 0.5 MgSO4 · 7 H2O, 1.0 % Na2SO4; carbonates – 0.15 Na2CO3, 0.30 % NaHCO3. The following straight-line regression equations have been constructed, allowing the use of model data to predict seed germination in practical agriculture, depending on the amount of increase / decrease in salt concentration in the soil.
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Claims 8
Claim · model-written summary
Increasing salt concentration decreased hemp seed germination.
- Taxon
- Cannabis sativa L.
Cannabis sativat0 - Treatment
- increase in the concentration of saltse0
- Outcome
- seed germinatione0
- Direction
- decreasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa L.”
e0“Hemp clearly responded to an increase in the concentration of salts in the nutrient medium, in particular, a decrease in seed germination was observed.”
Claim · model-written summary
Sulfate medium was most suitable for hemp cultivation.
- Taxon
- Cannabis sativa L.Cannabis sativat0
- Treatment
- nutrient medium with sulfatese0
- Outcome
- most suitable for cultivatione0
- Direction
- most suitablee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa L.”
e0“The most suitable for cultivation is the nutrient medium with sulfates, and the least – with carbonates (at relatively low concentrations).”
Claim · model-written summary
Carbonate medium was least suitable at relatively low concentrations.
- Taxon
- Cannabis sativa L.Cannabis sativat0
- Outcome
- least – with carbonatese0
- Direction
- leaste0
- Qualifier
- at relatively low concentrationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa L.”
e0“The most suitable for cultivation is the nutrient medium with sulfates, and the least – with carbonates (at relatively low concentrations).”
Claim · model-written summary
Salt screening test systems were developed and tested and were selective.
- Taxon
- Cannabis sativa L.Cannabis sativat0
- Outcome
- selective in their selectione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa L.”
e0“Test systems (including the concentrations of the studied salts) were developed and tested for salt stress screening. The test systems were selective in their selection.”
Claim · model-written summary
The chloride screening concentrations were 0.25 NaCl and 0.75 % MgCl2 · 6 H2O.
- Taxon
- Cannabis sativa L.Cannabis sativat0
- Outcome
- Selective concentrations of saltse0
- Value
- 0.25 NaCl, 0.75 % MgCl2 · 6 H2Oe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa L.”
e0“Selective concentrations of salts in the environment are determined as follows: chlorides – 0.25 NaCl, 0.75 % MgCl2 · 6 H2O”
Claim · model-written summary
The sulfate screening concentrations were 0.5 MgSO4 · 7 H2O and 1.0 % Na2SO4.
- Taxon
- Cannabis sativa L.Cannabis sativat0
- Outcome
- Selective concentrations of saltse0
- Value
- 0.5 MgSO4 · 7 H2O, 1.0 % Na2SO4e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa L.”
e0“Selective concentrations of salts in the environment are determined as follows: chlorides – 0.25 NaCl, 0.75 % MgCl2 · 6 H2O; sulfates – 0.5 MgSO4 · 7 H2O, 1.0 % Na2SO4”
Claim · model-written summary
The carbonate screening concentrations were 0.15 Na2CO3 and 0.30 % NaHCO3.
- Taxon
- Cannabis sativa L.Cannabis sativat0
- Outcome
- Selective concentrations of saltse0
- Value
- 0.15 Na2CO3, 0.30 % NaHCO3e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa L.”
e0“Selective concentrations of salts in the environment are determined as follows: chlorides – 0.25 NaCl, 0.75 % MgCl2 · 6 H2O; sulfates – 0.5 MgSO4 · 7 H2O, 1.0 % Na2SO4; carbonates – 0.15 Na2CO3, 0.30 % NaHCO3”
Claim · model-written summary
Straight-line regression equations were constructed to predict hemp germination from soil salt concentration change.
- Taxon
- Cannabis sativa L.Cannabis sativat0
- Outcome
- predict seed germinatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Cannabis sativa L.”
e0“The following straight-line regression equations have been constructed, allowing the use of model data to predict seed germination in practical agriculture, depending on the amount of increase / decrease in salt concentration in the soil.”
The same questions in other papers
salinity · growth · Cannabis sativaResults agree
2 papers in the same direction as this paper
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.