Abstract
Seed quality is crucial for boosting seed production and productivity. However, soybean seeds are inherently short lived, quickly loses viability even under optimal storage conditions. Therefore, maintaining their quality during storage is of prime importance. Keeping these in view a laboratory experiment was conducted to study the effect of modified atmospheric storage on seed quality of soybean (cv. DSb 34). The freshly harvested seeds with initial moisture content of 7.6% were stored in different modified atmospheric gaseous combinations for nine months from November 2023- August 2024. Results indicated that the seeds stored with gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bag maintained better seed quality in terms of germination (86.77%), shoot length (14.49 cm), root length (12.58 cm), dry weight(0.995g/ 10 seedlings), seedling vigour index I (2349) and II (86.34) upto the end of nine months storage period by recording higher total dehydrogenase (0.438 OD value), α- amylase (10.72 µmol of maltose released min-¹ ml-1 of enzyme) and catalase activity (1.364 µmol H₂O₂ decomposed minˉ¹gˉ¹ protein) compared to all other gaseous combinations and control. Hence, this technique can be used to maintain seed longevity with little deterioration, ensuring the quality of seeds even after extended storage period.
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Claims 9
Claim · model-written summary
The 90% CO2, 4% O2, 6% N2 atmosphere in polyethylene bags maintained better germination of soybean seed (86.77%).
- Taxon
- soybean
Glycine maxe1 - Material
- DSb 34e1
- Treatment
- gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bage2
- Outcome
- germinatione5
- Direction
- maintained bettere5
- Value
- 86.77%e5
- Comparator
- all other gaseous combinations and controle3
- Setting
- laboratorye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“seed quality of soybean (cv. DSb 34)”
e2“the seeds stored with gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bag maintained better seed quality”
e3“compared to all other gaseous combinations and control”
e4“a laboratory experiment was conducted”
e5“maintained better seed quality in terms of germination (86.77%)”
Claim · model-written summary
The same atmosphere maintained better shoot length of soybean seedlings (14.49 cm).
- Taxon
- soybean
Glycine maxe1 - Material
- DSb 34e1
- Treatment
- gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bage2
- Outcome
- shoot lengthe5
- Direction
- maintained bettere2
- Value
- 14.49 cme5
- Comparator
- all other gaseous combinations and controle3
- Setting
- laboratorye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“seed quality of soybean (cv. DSb 34)”
e2“the seeds stored with gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bag maintained better seed quality”
e3“compared to all other gaseous combinations and control”
e4“a laboratory experiment was conducted”
e5“shoot length (14.49 cm)”
Claim · model-written summary
The same atmosphere maintained better root length of soybean seedlings (12.58 cm).
- Taxon
- soybean
Glycine maxe1 - Material
- DSb 34e1
- Treatment
- gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bage2
- Outcome
- root lengthe5
- Direction
- maintained bettere2
- Value
- 12.58 cme5
- Comparator
- all other gaseous combinations and controle3
- Setting
- laboratorye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“seed quality of soybean (cv. DSb 34)”
e2“the seeds stored with gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bag maintained better seed quality”
e3“compared to all other gaseous combinations and control”
e4“a laboratory experiment was conducted”
e5“root length (12.58 cm)”
Claim · model-written summary
The same atmosphere maintained better seedling dry weight (0.995 g per 10 seedlings).
- Taxon
- soybean
Glycine maxe1 - Material
- DSb 34e1
- Treatment
- gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bage2
- Outcome
- dry weighte5
- Direction
- maintained bettere2
- Value
- 0.995g/ 10 seedlingse5
- Comparator
- all other gaseous combinations and controle3
- Setting
- laboratorye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“seed quality of soybean (cv. DSb 34)”
e2“the seeds stored with gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bag maintained better seed quality”
e3“compared to all other gaseous combinations and control”
e4“a laboratory experiment was conducted”
e5“dry weight(0.995g/ 10 seedlings)”
Claim · model-written summary
The same atmosphere maintained better seedling vigour index I (2349).
- Taxon
- soybean
Glycine maxe1 - Material
- DSb 34e1
- Treatment
- gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bage2
- Outcome
- seedling vigour indexe5
- Direction
- maintained bettere2
- Value
- 2349e5
- Comparator
- all other gaseous combinations and controle3
- Setting
- laboratorye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“seed quality of soybean (cv. DSb 34)”
e2“the seeds stored with gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bag maintained better seed quality”
e3“compared to all other gaseous combinations and control”
e4“a laboratory experiment was conducted”
e5“seedling vigour index I (2349) and II (86.34)”
Claim · model-written summary
The same atmosphere maintained better seedling vigour index II (86.34).
- Taxon
- soybean
Glycine maxe1 - Material
- DSb 34e1
- Treatment
- gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bage2
- Outcome
- seedling vigour indexe5
- Direction
- maintained bettere2
- Value
- 86.34e5
- Comparator
- all other gaseous combinations and controle3
- Setting
- laboratorye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“seed quality of soybean (cv. DSb 34)”
e2“the seeds stored with gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bag maintained better seed quality”
e3“compared to all other gaseous combinations and control”
e4“a laboratory experiment was conducted”
e5“seedling vigour index I (2349) and II (86.34)”
Claim · model-written summary
The same atmosphere gave higher total dehydrogenase activity (0.438 OD value).
- Taxon
- soybean
Glycine maxe1 - Material
- DSb 34e1
- Treatment
- gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bage2
- Outcome
- total dehydrogenasee5
- Direction
- highere5
- Value
- 0.438 OD valuee5
- Comparator
- all other gaseous combinations and controle3
- Setting
- laboratorye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“seed quality of soybean (cv. DSb 34)”
e2“the seeds stored with gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bag maintained better seed quality”
e3“compared to all other gaseous combinations and control”
e4“a laboratory experiment was conducted”
e5“higher total dehydrogenase (0.438 OD value)”
e6“compared to all other gaseous combinations and control”
Claim · model-written summary
The same atmosphere gave higher α-amylase activity (10.72 µmol of maltose released per min per ml of enzyme).
- Taxon
- soybean
Glycine maxe1 - Material
- DSb 34e1
- Treatment
- gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bage2
- Outcome
- α- amylasee5
- Direction
- highere6
- Value
- 10.72 µmol of maltose released min-¹ ml-1 of enzymee5
- Comparator
- all other gaseous combinations and controle3
- Setting
- laboratorye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“seed quality of soybean (cv. DSb 34)”
e2“the seeds stored with gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bag maintained better seed quality”
e3“compared to all other gaseous combinations and control”
e4“a laboratory experiment was conducted”
e5“α- amylase (10.72 µmol of maltose released min-¹ ml-1 of enzyme)”
e6“higher total dehydrogenase (0.438 OD value)”
Claim · model-written summary
The same atmosphere gave higher catalase activity (1.364 µmol H2O2 decomposed per min per g protein).
- Taxon
- soybean
Glycine maxe1 - Material
- DSb 34e1
- Treatment
- gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bage2
- Outcome
- catalase activitye5
- Direction
- highere6
- Value
- 1.364 µmol H₂O₂ decomposed minˉ¹gˉ¹ proteine5
- Comparator
- all other gaseous combinations and controle3
- Setting
- laboratorye4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Verbatim
e1“seed quality of soybean (cv. DSb 34)”
e2“the seeds stored with gaseous combination of 90 % CO2: 4 % O2: 6 % N2 in 700 gauge polyethylene bag maintained better seed quality”
e3“compared to all other gaseous combinations and control”
e4“a laboratory experiment was conducted”
e5“catalase activity (1.364 µmol H₂O₂ decomposed minˉ¹gˉ¹ protein)”
e6“higher total dehydrogenase (0.438 OD value)”
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