Abstract
To investigate the precise and efficient symbiosis between Sinorhizobium meliloti LL2 and different alfalfa varieties, we conducted experiments using eight alfalfa varieties along with the S. meliloti LL2. Our objective was to identify highly effective symbiotic combinations by analyzing differences in nodulation, nitrogen fixation, and biomass accumulation. The results revealed that Gannong NO.9 had higher values for single effective root nodule weight (1.30 mg) and the number of infected cells in root nodules (2795) compared to other varieties (p < 0.05). Additionally, Gannong NO.9 exhibited the highest nitrogenase activity (0.91 μmol·g−1·h−1), nitrogen fixation percentage (67.16%), and amount of nitrogen fixation (18.80 mg/pot). Moreover, there was a significant 26.50% increase in aboveground tissue nitrogen accumulation compared to the control check (CK) (p < 0.05). Furthermore, underground tissue showed excellent values for nitrogen accumulation (35.68 mg/plant) and crude protein content (17.75%) when compared with other treatments. The growth of plants was demonstrated by the combined impact of nodulation and nitrogen fixation. The distribution of biomass after nitrogen fixation was compared to the control group (p < 0.05) to investigate accumulation. The eight combinations of symbiotic nitrogen fixation (SNF) were classified into six distinct types based on their significantly different biomass growth rates compared to CK. ① Aboveground accumulation type: Gannong NO.9 (there was a 24.31% increase in aboveground dry weight); ② aboveground and underground accumulation type: Qingshui (the aboveground dry weight increased by 135.94%, while the underground dry weight grew by 35.26%); ③ aboveground accumulation, underground depletion type: Gannong NO.5 ( ); ④ zero-growth type (there was no significant difference in dry weights, both above and below ground, compared to CK): WL168HQ, WL319HQ and Longzhong; ⑤ aboveground and underground depletion type: WL298HQ (the aboveground dry weight decreased by 29.29%, while the underground dry weight decreased by 20.23%); ⑥ underground depletion type: Gannong NO.3 (the underground dry weight showed a decrease of 34.49%); no type with aboveground consumption and underground accumulation was found. The study clarified the optimal combination of LL2 and Gannong NO.9, finding that biomass accumulation after symbiotic nitrogen fixation is variety-dependent.
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Claims 16
Claim · model-written summary
Gannong NO.9 had higher effective root nodule weight of 1.30 mg than other varieties.
- Taxon
- alfalfa
Medicago sativat0 - Material
- Gannong NO.9e0
- Outcome
- single effective root nodule weighte0
- Direction
- highere0
- Value
- 1.30 mge0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gannong NO.9 had higher values for single effective root nodule weight (1.30 mg)”
t0“Alfalfa”
Claim · model-written summary
Gannong NO.9 had 2795 infected root nodule cells, higher than other varieties.
- Taxon
- alfalfaMedicago sativat0
- Material
- Gannong NO.9e1
- Outcome
- number of infected cells in root nodulese0
- Value
- 2795e0
- Comparator
- other varietiese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the number of infected cells in root nodules (2795) compared to other varieties (p < 0.05)”
t0“Alfalfa”
e1“Gannong NO.9 had higher values for single effective root nodule weight (1.30 mg)”
Claim · model-written summary
Gannong NO.9 had highest nitrogenase activity of 0.91 μmol·g−1·h−1.
- Taxon
- alfalfaMedicago sativat0
- Material
- Gannong NO.9e0
- Outcome
- nitrogenase activitye0
- Direction
- higheste0
- Value
- 0.91 μmol·g−1·h−1e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gannong NO.9 exhibited the highest nitrogenase activity (0.91 μmol·g−1·h−1)”
t0“Alfalfa”
Claim · model-written summary
Gannong NO.9 had highest nitrogen fixation percentage of 67.16%.
- Taxon
- alfalfaMedicago sativat0
- Material
- Gannong NO.9e1
- Outcome
- nitrogen fixation percentagee0
- Value
- 67.16%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nitrogen fixation percentage (67.16%)”
t0“Alfalfa”
e1“Gannong NO.9 had higher values for single effective root nodule weight (1.30 mg)”
Claim · model-written summary
Gannong NO.9 had highest nitrogen fixation amount of 18.80 mg/pot.
- Taxon
- alfalfaMedicago sativat0
- Material
- Gannong NO.9e1
- Outcome
- amount of nitrogen fixatione0
- Value
- 18.80 mg/pote0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“amount of nitrogen fixation (18.80 mg/pot)”
t0“Alfalfa”
e1“Gannong NO.9 had higher values for single effective root nodule weight (1.30 mg)”
Claim · model-written summary
Aboveground nitrogen accumulation increased significantly by 26.50% versus CK.
- Taxon
- alfalfaMedicago sativat0
- Material
- Gannong NO.9e1
- Treatment
- Sinorhizobium meliloti LL2t1
- Outcome
- aboveground tissue nitrogen accumulatione0
- Direction
- increasee0
- Value
- 26.50%e0
- Comparator
- control check (CK)e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“significant 26.50% increase in aboveground tissue nitrogen accumulation compared to the control check (CK)”
t0“Alfalfa”
e1“Gannong NO.9 had higher values for single effective root nodule weight (1.30 mg)”
t1“Sinorhizobium meliloti LL2”
Claim · model-written summary
Underground tissue nitrogen accumulation was 35.68 mg/plant.
- Taxon
- alfalfaMedicago sativat0
- Organ / stage
- underground tissuee1
- Outcome
- nitrogen accumulatione0
- Value
- 35.68 mg/plante0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“nitrogen accumulation (35.68 mg/plant)”
t0“Alfalfa”
e1“underground tissue”
Claim · model-written summary
Underground tissue crude protein content was 17.75%.
- Taxon
- alfalfaMedicago sativat0
- Organ / stage
- underground tissuee1
- Outcome
- crude protein contente0
- Value
- 17.75%e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“crude protein content (17.75%)”
t0“Alfalfa”
e1“underground tissue”
Claim · model-written summary
Gannong NO.9 aboveground dry weight increased 24.31%.
- Taxon
- alfalfaMedicago sativat0
- Material
- Gannong NO.9e0
- Treatment
- Sinorhizobium meliloti LL2t1
- Outcome
- aboveground dry weighte0
- Direction
- increasee0
- Value
- 24.31%e0
- Comparator
- CKe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gannong NO.9 (there was a 24.31% increase in aboveground dry weight)”
t0“Alfalfa”
t1“Sinorhizobium meliloti LL2”
e1“biomass growth rates compared to CK”
Claim · model-written summary
Qingshui aboveground dry weight increased 135.94%.
- Taxon
- alfalfaMedicago sativat0
- Material
- Qingshuie0
- Treatment
- Sinorhizobium meliloti LL2t1
- Outcome
- aboveground dry weighte0
- Direction
- increasede0
- Value
- 135.94%e0
- Comparator
- CKe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Qingshui (the aboveground dry weight increased by 135.94%”
t0“Alfalfa”
t1“Sinorhizobium meliloti LL2”
e1“biomass growth rates compared to CK”
Claim · model-written summary
Qingshui underground dry weight increased 35.26%.
- Taxon
- alfalfaMedicago sativat0
- Material
- Qingshuie1
- Treatment
- Sinorhizobium meliloti LL2t1
- Outcome
- underground dry weighte0
- Direction
- grewe0
- Value
- 35.26%e0
- Comparator
- CKe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“underground dry weight grew by 35.26%”
t0“Alfalfa”
e1“Qingshui (the aboveground dry weight increased by 135.94%”
t1“Sinorhizobium meliloti LL2”
e2“biomass growth rates compared to CK”
Claim · model-written summary
WL168HQ, WL319HQ and Longzhong dry weights did not significantly differ from CK.
- Taxon
- alfalfaMedicago sativat0
- Material
- WL168HQ, WL319HQ and Longzhonge0
- Treatment
- Sinorhizobium meliloti LL2t1
- Outcome
- dry weightse0
- Direction
- no significant differencee0
- Comparator
- CKe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“there was no significant difference in dry weights, both above and below ground, compared to CK): WL168HQ, WL319HQ and Longzhong”
t0“Alfalfa”
t1“Sinorhizobium meliloti LL2”
Claim · model-written summary
WL298HQ aboveground dry weight decreased 29.29%.
- Taxon
- alfalfaMedicago sativat0
- Material
- WL298HQe0
- Treatment
- Sinorhizobium meliloti LL2t1
- Outcome
- aboveground dry weighte0
- Direction
- decreasede0
- Value
- 29.29%e0
- Comparator
- CKe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“WL298HQ (the aboveground dry weight decreased by 29.29%”
t0“Alfalfa”
t1“Sinorhizobium meliloti LL2”
e1“biomass growth rates compared to CK”
Claim · model-written summary
WL298HQ underground dry weight decreased 20.23%.
- Taxon
- alfalfaMedicago sativat0
- Material
- WL298HQe1
- Treatment
- Sinorhizobium meliloti LL2t1
- Outcome
- underground dry weighte0
- Direction
- decreasede0
- Value
- 20.23%e0
- Comparator
- CKe2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“underground dry weight decreased by 20.23%”
t0“Alfalfa”
e1“WL298HQ (the aboveground dry weight decreased by 29.29%”
t1“Sinorhizobium meliloti LL2”
e2“biomass growth rates compared to CK”
Claim · model-written summary
Gannong NO.3 underground dry weight decreased 34.49%.
- Taxon
- alfalfaMedicago sativat0
- Material
- Gannong NO.3e0
- Treatment
- Sinorhizobium meliloti LL2t1
- Outcome
- underground dry weighte0
- Direction
- decreasee0
- Value
- 34.49%e0
- Comparator
- CKe1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gannong NO.3 (the underground dry weight showed a decrease of 34.49%)”
t0“Alfalfa”
t1“Sinorhizobium meliloti LL2”
e1“biomass growth rates compared to CK”
Claim · model-written summary
LL2 and Gannong NO.9 was the optimal symbiotic combination.
- Taxon
- alfalfaMedicago sativat0
- Material
- Gannong NO.9e0
- Outcome
- optimal combinatione0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The study clarified the optimal combination of LL2 and Gannong NO.9”
t0“Alfalfa”
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