Abstract
As the only evergreen relict species in the desert environment of western China, Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE), but the potential of DSE to alleviate the adverse effects of drought on seedling roots remains uncertain. This study examined the effects of DSE on root growth, physiology and transcriptome of A . mongolicus under drought stress. Drought drastically reduced root biomass by 47.7%, while all DSE strains established positive symbiosis with A.mongolicus , with G.hyphopodioides having the most pronounced promoting effect. Inoculation with G. hyphopodioides alleviated drought stress injury by increasing CAT activity, AsA content and soluble sugar content in the roots, with a significant reduction in MDA accumulation by 97.7%. G. hyphopodioides also significantly increased zeatin and brassinosteroid contents, which in turn regulated the root structure and increased root activity, resulting in a 208.6% increase in root biomass. Transcriptome analysis screened 1246 differentially expressed genes (542 up-regulated and 704 down-regulated) between G. hyphopodioides inoculation under drought treatment, mainly associated with phenylpropanoid biosynthesis, ascorbic acid and aldehyde metabolism, hormone synthesis and signalling, sucrose and starch metabolism, and vitamin B6 metabolism, and further investigated and identified key potential genes and transcription factors ( DREB , ERF, NAC , MYB , C2H2 ). These findings reveal the physiological and molecular mechanisms by which DSE symbiosis improves the drought resistance of A. mongolicus seedlings, providing valuable guidance on the use of DSE resources to promote ecological construction and production of desert plants. • DSE reduced the oxidative stress damage of A.mongolicus root under drought stress. • DSE regulated hormone synthesis and root architecture under drought stress. • DSE increased the root activity and biomass of A.mongolicus under drought stress. • DSE activates the expression of transcription factors under drought stress. • DSE inoculation can be used for A.mongolicus cultivation in dryland.
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Claims 16
Claim · model-written summary
Drought reduced Ammopiptanthus mongolicus root biomass by 47.7%.
- Taxon
- Ammopiptanthus mongolicus
Ammopiptanthus mongolicuse0 - Treatment
- Droughte1
- Outcome
- root biomasse1
- Direction
- reducede1
- Value
- 47.7%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“Drought drastically reduced root biomass by 47.7%”
Claim · model-written summary
All DSE strains established positive symbiosis with A. mongolicus.
- Taxon
- A.mongolicusAmmopiptanthus mongolicuse1
- Interactor
- DSE strainse1symbiont
- Outcome
- positive symbiosise1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“all DSE strains established positive symbiosis with A.mongolicus , with G.hyphopodioides having the most pronounced promoting effect”
Claim · model-written summary
G. hyphopodioides had the most pronounced promoting effect among the DSE strains on A. mongolicus.
- Taxon
- A.mongolicusAmmopiptanthus mongolicuse1
- Interactor
- G.hyphopodioidese1symbiont
- Outcome
- promoting effecte1
- Qualifier
- most pronouncede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“all DSE strains established positive symbiosis with A.mongolicus , with G.hyphopodioides having the most pronounced promoting effect”
Claim · model-written summary
Inoculation with G. hyphopodioides under drought increased CAT activity, AsA content and soluble sugar content in the roots.
- Taxon
- Ammopiptanthus mongolicusAmmopiptanthus mongolicuse0
- Organ / stage
- rootse1
- Treatment
- Inoculation with G. hyphopodioidese1
- Interactor
- G. hyphopodioidese1symbiont
- Outcome
- CAT activity, AsA content and soluble sugar contente1
- Direction
- increasinge1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“Inoculation with G. hyphopodioides alleviated drought stress injury by increasing CAT activity, AsA content and soluble sugar content in the roots”
Claim · model-written summary
Inoculation with G. hyphopodioides under drought significantly reduced MDA accumulation by 97.7%.
- Taxon
- Ammopiptanthus mongolicusAmmopiptanthus mongolicuse0
- Treatment
- Inoculation with G. hyphopodioidese1
- Interactor
- G. hyphopodioidese1symbiont
- Outcome
- MDA accumulatione2
- Direction
- reductione2
- Value
- 97.7%e2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“Inoculation with G. hyphopodioides alleviated drought stress injury by increasing CAT activity, AsA content and soluble sugar content in the roots”
e2“with a significant reduction in MDA accumulation by 97.7%”
Claim · model-written summary
G. hyphopodioides significantly increased zeatin and brassinosteroid contents in A. mongolicus roots.
- Taxon
- Ammopiptanthus mongolicusAmmopiptanthus mongolicuse0
- Treatment
- Inoculation with G. hyphopodioidese2
- Interactor
- G. hyphopodioidese1symbiont
- Outcome
- zeatin and brassinosteroid contentse1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“G. hyphopodioides also significantly increased zeatin and brassinosteroid contents, which in turn regulated the root structure and increased root activity, resulting in a 208.6% increase in root biomass”
e2“Inoculation with G. hyphopodioides alleviated drought stress injury”
Claim · model-written summary
G. hyphopodioides inoculation increased Ammopiptanthus mongolicus root biomass by 208.6%.
- Taxon
- Ammopiptanthus mongolicusAmmopiptanthus mongolicuse0
- Treatment
- Inoculation with G. hyphopodioidese2
- Interactor
- G. hyphopodioidese1symbiont
- Outcome
- root biomasse1
- Direction
- increasee1
- Value
- 208.6%e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“G. hyphopodioides also significantly increased zeatin and brassinosteroid contents, which in turn regulated the root structure and increased root activity, resulting in a 208.6% increase in root biomass”
e2“Inoculation with G. hyphopodioides alleviated drought stress injury”
Claim · model-written summary
G. hyphopodioides inoculation increased root activity of A. mongolicus.
- Taxon
- Ammopiptanthus mongolicusAmmopiptanthus mongolicuse0
- Treatment
- Inoculation with G. hyphopodioidese2
- Interactor
- G. hyphopodioidese1symbiont
- Outcome
- root activitye1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“G. hyphopodioides also significantly increased zeatin and brassinosteroid contents, which in turn regulated the root structure and increased root activity, resulting in a 208.6% increase in root biomass”
e2“Inoculation with G. hyphopodioides alleviated drought stress injury”
Claim · model-written summary
Zeatin and brassinosteroid contents are proposed to regulate root structure in A. mongolicus.
- Taxon
- Ammopiptanthus mongolicusAmmopiptanthus mongolicuse0
- Compound
- zeatin and brassinosteroide1
- Outcome
- root structuree1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“G. hyphopodioides also significantly increased zeatin and brassinosteroid contents, which in turn regulated the root structure and increased root activity, resulting in a 208.6% increase in root biomass”
Claim · model-written summary
Transcriptome analysis found 1246 differentially expressed genes between G. hyphopodioides inoculation under drought and the comparison.
- Taxon
- Ammopiptanthus mongolicusAmmopiptanthus mongolicuse0
- Treatment
- G. hyphopodioides inoculation under drought treatmente1
- Interactor
- G. hyphopodioidese1symbiont
- Outcome
- differentially expressed genese1
- Value
- 1246e1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“Transcriptome analysis screened 1246 differentially expressed genes (542 up-regulated and 704 down-regulated) between G. hyphopodioides inoculation under drought treatment”
Claim · model-written summary
G. hyphopodioides inoculation under drought up-regulated 542 genes in A. mongolicus.
- Taxon
- Ammopiptanthus mongolicusAmmopiptanthus mongolicuse0
- Treatment
- G. hyphopodioides inoculation under drought treatmente1
- Outcome
- differentially expressed genese1
- Direction
- up-regulatede1
- Value
- 542e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“Transcriptome analysis screened 1246 differentially expressed genes (542 up-regulated and 704 down-regulated) between G. hyphopodioides inoculation under drought treatment”
Claim · model-written summary
G. hyphopodioides inoculation under drought down-regulated 704 genes in A. mongolicus.
- Taxon
- Ammopiptanthus mongolicusAmmopiptanthus mongolicuse0
- Treatment
- G. hyphopodioides inoculation under drought treatmente1
- Outcome
- differentially expressed genese1
- Direction
- down-regulatede1
- Value
- 704e1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“Transcriptome analysis screened 1246 differentially expressed genes (542 up-regulated and 704 down-regulated) between G. hyphopodioides inoculation under drought treatment”
Claim · model-written summary
Differentially expressed genes are associated with phenylpropanoid biosynthesis, ascorbic acid and aldehyde metabolism, and hormone synthesis and signalling.
- Taxon
- Ammopiptanthus mongolicusAmmopiptanthus mongolicuse0
- Treatment
- G. hyphopodioides inoculation under drought treatmente1
- Outcome
- phenylpropanoid biosynthesis, ascorbic acid and aldehyde metabolism, hormone synthesis and signallinge2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“Transcriptome analysis screened 1246 differentially expressed genes (542 up-regulated and 704 down-regulated) between G. hyphopodioides inoculation under drought treatment”
e2“mainly associated with phenylpropanoid biosynthesis, ascorbic acid and aldehyde metabolism, hormone synthesis and signalling, sucrose and starch metabolism, and vitamin B6 metabolism”
Claim · model-written summary
Differentially expressed genes are associated with sucrose and starch metabolism and vitamin B6 metabolism.
- Taxon
- Ammopiptanthus mongolicusAmmopiptanthus mongolicuse0
- Treatment
- G. hyphopodioides inoculation under drought treatmente1
- Outcome
- sucrose and starch metabolism, and vitamin B6 metabolisme2
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“Transcriptome analysis screened 1246 differentially expressed genes (542 up-regulated and 704 down-regulated) between G. hyphopodioides inoculation under drought treatment”
e2“mainly associated with phenylpropanoid biosynthesis, ascorbic acid and aldehyde metabolism, hormone synthesis and signalling, sucrose and starch metabolism, and vitamin B6 metabolism”
Claim · model-written summary
Key potential genes and transcription factors DREB, ERF and NAC were identified in A. mongolicus under drought.
- Taxon
- Ammopiptanthus mongolicusAmmopiptanthus mongolicuse0
- Gene
- DREB , ERF, NACe1
- Outcome
- key potential genes and transcription factorse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“further investigated and identified key potential genes and transcription factors ( DREB , ERF, NAC , MYB , C2H2 )”
Claim · model-written summary
Key potential transcription factors MYB and C2H2 were identified in A. mongolicus under drought.
- Taxon
- Ammopiptanthus mongolicusAmmopiptanthus mongolicuse0
- Gene
- MYB , C2H2e1
- Outcome
- key potential genes and transcription factorse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Ammopiptanthus mongolicus (Leguminosae) roots is colonized with dark septate endophytes (DSE)”
e1“further investigated and identified key potential genes and transcription factors ( DREB , ERF, NAC , MYB , C2H2 )”
The same questions in other papers
drought · growth · Ammopiptanthus mongolicusResults agree
1 paper in the same direction as this paper
- negative
inoculation · growth · Ammopiptanthus mongolicusResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.