Abstract
In this comprehensive study, the synergetic impact of sodium azide and EMS mutagenic treatments on ‘Parbhani Kranti’ okra seeds and plants were rigorously examined. The study employed okra seeds in a CRD, implementing five treatments (T0 to T4) with varied concentrations of Sodium Azide and EMS. The primary goal was to assess genetic purity, covering morphological parameters, DUS tests; Physiological observations included chlorophyll, RW content and molecular analysis by using SSR Marker. Morphological parameters, including shoot, root, seedling length, and leaf area were adversely affected, with T4 showing the most stunted growth.Flowering delay and altered fruit characteristics were also observed, correlating with mutagen concentrations. The findings of this research reveal that seeds subjected to mutagenic treatments exhibited significantly reduced germination rates when compared to the control. The most substantial reduction, at 70%, were observed in seeds treated (T4) and was detrimentally influenced by these mutagenic interventions. The increase chlorophyll (mean 1.47) and RWC (mean 47.06) complex plant responses to mutagenic elements. Potential adaptive or tolerance mechanisms, including increased chlorophyll production and water retention, may be activated to mitigate stress. Genetic purity assessments exposed variations in DNA content and quality across different treatments. Among the two SSR marker differentiated allelic size of 260 and 300 bp. The allelic sizes are specific to the seed and pollen of the hybrid. These findings hold implications for developing novel okra cultivars and ensuring genetic purity in hybrid seed production, contributing to agricultural sustainability.
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Claims 9
Claim · model-written summary
Mutagen treatment adversely affected shoot, root and seedling length.
- Taxon
- Okra
.jpg?width=60)
Abelmoschus esculentust0 - Material
- T4e1
- Treatment
- Sodium Azide and EMSe0
- Outcome
- shoot, root, seedling lengthe1
- Direction
- adversely affectede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
.jpg?width=500)
Verbatim
e0“Sodium Azide and EMS”
e1“Morphological parameters, including shoot, root, seedling length, and leaf area were adversely affected, with T4 showing the most stunted growth”
t0“Okra Seedling”
Claim · model-written summary
Mutagen treatment adversely affected leaf area.
- Taxon
- OkraAbelmoschus esculentust0
- Treatment
- Sodium Azide and EMSe0
- Outcome
- leaf areae1
- Direction
- adversely affectede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Sodium Azide and EMS”
e1“leaf area were adversely affected, with T4 showing the most stunted growth”
t0“Okra Seedling”
Claim · model-written summary
Flowering was delayed with mutagen concentration.
- Taxon
- OkraAbelmoschus esculentust0
- Treatment
- mutagen concentrationse0
- Outcome
- Flowering delaye0
- Direction
- delaye0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Flowering delay and altered fruit characteristics were also observed, correlating with mutagen concentrations”
t0“Okra Seedling”
Claim · model-written summary
Fruit characteristics changed with mutagen concentration.
- Taxon
- OkraAbelmoschus esculentust0
- Treatment
- mutagen concentrationse0
- Outcome
- fruit characteristicse0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“altered fruit characteristics were also observed, correlating with mutagen concentrations”
t0“Okra Seedling”
Claim · model-written summary
Mutagen treated seeds had significantly lower germination, with T4 showing a 70% reduction.
- Taxon
- OkraAbelmoschus esculentust0
- Material
- T4e0
- Treatment
- mutagenic treatmentse0
- Outcome
- germination ratese0
- Direction
- reducede0
- Value
- 70%e0
- Comparator
- the controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“seeds subjected to mutagenic treatments exhibited significantly reduced germination rates when compared to the control. The most substantial reduction, at 70%, were observed in seeds treated (T4)”
t0“Okra Seedling”
Claim · model-written summary
Chlorophyll increased to a reported mean of 1.47.
- Taxon
- OkraAbelmoschus esculentust0
- Outcome
- chlorophylle0
- Direction
- increasee0
- Value
- 1.47e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The increase chlorophyll (mean 1.47)”
t0“Okra Seedling”
Claim · model-written summary
RWC had a reported mean of 47.06.
- Taxon
- OkraAbelmoschus esculentust0
- Outcome
- RWCe0
- Value
- 47.06e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“RWC (mean 47.06)”
t0“Okra Seedling”
Claim · model-written summary
DNA content and quality varied across treatments.
- Taxon
- OkraAbelmoschus esculentust0
- Treatment
- different treatmentse0
- Outcome
- DNA content and qualitye0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genetic purity assessments exposed variations in DNA content and quality across different treatments”
t0“Okra Seedling”
Claim · model-written summary
Two SSR markers differentiated hybrid seed and pollen alleles of 260 and 300 bp.
- Taxon
- OkraAbelmoschus esculentust0
- Organ / stage
- seed and pollene0
- Outcome
- allelic sizee0
- Value
- 260 and 300 bpe0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Among the two SSR marker differentiated allelic size of 260 and 300 bp. The allelic sizes are specific to the seed and pollen of the hybrid”
t0“Okra Seedling”
The same questions in other papers
sodium azide · growth · Abelmoschus esculentus (okra)Results differ
1 paper in the same direction as this paper · 1 in the opposite direction
- positive
- negative
ethyl methanesulfonate · growth · Abelmoschus esculentus (okra)Results agree
2 papers in the same direction as this paper
- negative
- negative
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.