Abstract
Soybean is particularly known for accumulating saponins in its seeds. This study aimed to identify a causal gene to control an increase in Ab-γg saponin in PE1607 from an EMS-treated population of the soybean cultivar Pungsannamul. Segregation analysis in F2 seeds verified that a single recessive allele controlled the increased Ab-γg saponin in PE1607. Bulk segregant analysis and mutant individuals identified the candidate region, containing the previously reported Sg-3 (Glyma.10G104700) gene, encoding a glucosyltransferase responsible for conjugating glucose as the third sugar at the C-3 position of the aglycone. NGS identified SNPs in the upstream of the Sg-3 gene, designated as the sg-3b allele. Expression analysis revealed that PE1607 exhibited a threefold decrease in Sg-3 expression in the hypocotyls compared to the Pungsannamul. Moreover, Sg-3 expressions significantly differed between the hypocotyls and cotyledons in developing seeds, with relatively low expression observed in the cotyledons. The results conclude that sg-3b allele may contribute to the reduced Sg-3 expression, resulting in an increase in Ab-γg saponin in PE1607. In addition, in the cotyledons, DDMP-βg and DDMP-βa saponins are present, containing rhamnose instead of glucose as the third sugar at the C-3 position of aglycone. This suggests that Sg-3, known as glucosyltransferase, does not significantly contribute to saponin biosynthesis in cotyledons.
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Claims 8
Claim · model-written summary
A single recessive allele controlled the increased Ab-γg saponin in mutant PE1607.
- Taxon
- Soybean
Glycine maxt0 - Material
- PE1607e1
- Gene
- single recessive allelee1
- Outcome
- increased Ab-γg saponine1
- Qualifier
- Segregation analysis in F2 seedse1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean (Glycine max (L.) Merr.)”
e1“Segregation analysis in F2 seeds verified that a single recessive allele controlled the increased Ab-γg saponin in PE1607”
Claim · model-written summary
Bulk segregant analysis identified a candidate region containing the Sg-3 glucosyltransferase gene.
- Taxon
- SoybeanGlycine maxt0
- Gene
- Sg-3e2
- Outcome
- candidate regione2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean (Glycine max (L.) Merr.)”
e2“Bulk segregant analysis and mutant individuals identified the candidate region, containing the previously reported Sg-3 (Glyma.10G104700) gene”
Claim · model-written summary
NGS identified SNPs upstream of Sg-3, designated as the sg-3b allele.
- Taxon
- SoybeanGlycine maxt0
- Gene
- sg-3b allelee3
- Outcome
- SNPse3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean (Glycine max (L.) Merr.)”
e3“NGS identified SNPs in the upstream of the Sg-3 gene, designated as the sg-3b allele”
Claim · model-written summary
Mutant PE1607 showed a threefold decrease in Sg-3 expression in hypocotyls compared with Pungsannamul.
- Taxon
- SoybeanGlycine maxt0
- Material
- PE1607e4
- Organ / stage
- hypocotylse4
- Gene
- Sg-3e4
- Outcome
- Sg-3 expressione4
- Direction
- decreasee4
- Value
- threefolde4
- Comparator
- Pungsannamule4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean (Glycine max (L.) Merr.)”
e4“Expression analysis revealed that PE1607 exhibited a threefold decrease in Sg-3 expression in the hypocotyls compared to the Pungsannamul”
Claim · model-written summary
Sg-3 expression differed significantly between hypocotyls and cotyledons of developing seeds, with relatively low expression in cotyledons.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- developing seedse5
- Gene
- Sg-3e5
- Outcome
- Sg-3 expressionse5
- Direction
- relatively lowe5
- Comparator
- hypocotylse5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean (Glycine max (L.) Merr.)”
e5“Sg-3 expressions significantly differed between the hypocotyls and cotyledons in developing seeds, with relatively low expression observed in the cotyledons”
Claim · model-written summary
The sg-3b allele may contribute to reduced Sg-3 expression, resulting in more Ab-γg saponin in PE1607.
- Taxon
- SoybeanGlycine maxt0
- Gene
- sg-3b allelee6
- Outcome
- increase in Ab-γg saponine6
- Qualifier
- reduced Sg-3 expressione6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean (Glycine max (L.) Merr.)”
e6“The results conclude that sg-3b allele may contribute to the reduced Sg-3 expression, resulting in an increase in Ab-γg saponin in PE1607”
Claim · model-written summary
In cotyledons, DDMP-βg and DDMP-βa saponins are present with rhamnose instead of glucose as the third sugar at the C-3 position of the aglycone.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- cotyledonse7
- Outcome
- third sugar at the C-3 positione7
- Qualifier
- rhamnose instead of glucosee7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean (Glycine max (L.) Merr.)”
e7“in the cotyledons, DDMP-βg and DDMP-βa saponins are present, containing rhamnose instead of glucose as the third sugar at the C-3 position of aglycone”
Claim · model-written summary
Sg-3 does not significantly contribute to saponin biosynthesis in cotyledons.
- Taxon
- SoybeanGlycine maxt0
- Organ / stage
- cotyledonse7
- Gene
- Sg-3e7
- Outcome
- saponin biosynthesise7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“Soybean (Glycine max (L.) Merr.)”
e7“This suggests that Sg-3, known as glucosyltransferase, does not significantly contribute to saponin biosynthesis in cotyledons”