Abstract
The ABA receptor pyrabactin resistance 1-like (PYR/PYL) protein family is among the key regulators of plant growth and development, participating in ABA signaling. However, information on this family in strawberry (Fragaria × ananassa), an important fleshy fruit crop worldwide, is limited. In the present work, seven homologs of Arabidopsis PYL were first identified and characterized from Fragaria × ananassa. Furthermore, a comprehensive analysis of the FaPYLs was performed, including their phylogenetic relationships, gene structures, chromosomal locations, and conserved domains. The qRT–PCR analysis showed that FaPYL genes had differential expression patterns between fruit ripening stages, and most of them were transcribed in the SG (small green fruits,) and Wh (white fruits) stages of fruit ripening. Treatment of strawberry fruits with ABA and JA changed the expression of all FaPYLs in a gene-specific manner, depending on the berry ripening stage; at the turning stage, only the expression levels of FaPYL3 and FaPYL4 increased in response to ABA. Promoter analysis showed that most FaPYL genes contain cis-acting elements that respond to stress, light, and phytohormones and are associated with tissue-specific expression. Additionally, FaPYL3 and FaPYL4 were further functionally characterized using transient expression technology. Overexpression of FaPYL3 and FaPYL4 promoted strawberry fruit ripening, which positively regulated FaSnRK2.2 and FaNCED1 transcripts. Our results highlight the great potential of FaPYL genes in breeding genetically modified strawberry varieties with accelerated fruit ripening.
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Claims 8
Claim · model-written summary
Seven Arabidopsis PYL homologs were identified in strawberry.
- Taxon
- Fragaria × ananassa
_single2.jpg?width=60)
Fragaria ×ananassae0 - Gene
- Arabidopsis PYLe0
- Outcome
- homologs of Arabidopsis PYLe0
- Value
- seven homologse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_single2.jpg?width=500)
Verbatim
e0“seven homologs of Arabidopsis PYL were first identified and characterized from Fragaria × ananassa”
Claim · model-written summary
FaPYL expression differed among strawberry fruit ripening stages, with most transcribed at SG and Wh stages.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- fruit ripening stagese0
- Gene
- FaPYL genese0
- Outcome
- differential expression patternse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“FaPYL genes had differential expression patterns between fruit ripening stages, and most of them were transcribed in the SG (small green fruits,) and Wh (white fruits) stages of fruit ripening”
t0“Strawberry”
Claim · model-written summary
ABA and JA treatments changed FaPYL expression according to gene and ripening stage.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- ABA and JAe0
- Gene
- FaPYLse0
- Outcome
- expression of all FaPYLse0
- Direction
- changede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Treatment of strawberry fruits with ABA and JA changed the expression of all FaPYLs in a gene-specific manner, depending on the berry ripening stage”
Claim · model-written summary
At the turning stage, ABA increased only FaPYL3 and FaPYL4 expression.
- Taxon
- strawberryFragaria ×ananassat0
- Organ / stage
- turning stagee0
- Treatment
- ABAe0
- Gene
- FaPYL3 and FaPYL4e0
- Outcome
- expression levels of FaPYL3 and FaPYL4e0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“at the turning stage, only the expression levels of FaPYL3 and FaPYL4 increased in response to ABA”
t0“Strawberry”
Claim · model-written summary
Most FaPYL genes contained cis-acting elements responsive to stress, light and phytohormones and associated with tissue-specific expression.
- Taxon
- strawberryFragaria ×ananassat0
- Gene
- FaPYL genese0
- Outcome
- cis-acting elementse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“most FaPYL genes contain cis-acting elements that respond to stress, light, and phytohormones and are associated with tissue-specific expression”
t0“Strawberry”
Claim · model-written summary
FaPYL3 overexpression promoted strawberry fruit ripening.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- Overexpression of FaPYL3e0
- Gene
- FaPYL3e0
- Outcome
- fruit ripeninge0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of FaPYL3 and FaPYL4 promoted strawberry fruit ripening, which positively regulated FaSnRK2.2 and FaNCED1 transcripts”
Claim · model-written summary
FaPYL4 overexpression promoted strawberry fruit ripening.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- FaPYL4e0
- Gene
- FaPYL4e0
- Outcome
- fruit ripeninge0
- Direction
- promotede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of FaPYL3 and FaPYL4 promoted strawberry fruit ripening, which positively regulated FaSnRK2.2 and FaNCED1 transcripts”
Claim · model-written summary
FaPYL3 and FaPYL4 overexpression positively regulated FaSnRK2.2 and FaNCED1 transcripts.
- Taxon
- strawberryFragaria ×ananassae0
- Treatment
- Overexpression of FaPYL3 and FaPYL4e0
- Gene
- FaSnRK2.2 and FaNCED1e0
- Outcome
- FaSnRK2.2 and FaNCED1 transcriptse0
- Direction
- positively regulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Overexpression of FaPYL3 and FaPYL4 promoted strawberry fruit ripening, which positively regulated FaSnRK2.2 and FaNCED1 transcripts”
The same questions in other papers
(+)-abscisic acid · molecular: expression · Fragaria x ananassa (strawberry)Positive and negative results
7 papers in the same direction as this paper · 2 in the opposite direction
- no effect
- no effect
- positive
6 papers more in this group
See all 10 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.