Abstract
Serotonin N-acetyltransferase (SNAT) is a crucial enzyme in the melatonin synthesis pathway, playing an essential role in both melatonin biosynthesis and plant resistance to abiotic stress. A bioinformatics approach was employed to identify the members of the citrus SNAT gene family and to analyze their physicochemical properties, gene structure, conserved domains, phylogenetic relationships, and promoter cis-acting elements. Additionally, the tissue-specific expression of trifoliate orange SNAT family members and their expression patterns under stress conditions were examined. This study identified 21 members of the SNAT gene family across five citrus genomes, distributed over five chromosomes, with the majority predicted to localize within chloroplasts. These genes were characterized by having between 1 and 8 exons, 0 and 7 introns, 1 and 2 conserved domains, and 5 and 8 motifs. Phylogenetic analysis classified the genes into four subgroups, demonstrating significant collinearity with SNAT genes in rice. Analysis of the promoter regions revealed 32 cis-acting elements, with those responsive to light, abscisic acid, and drought being the most common. Expression analysis of SNAT genes in trifoliate orange indicated tissue specificity, with the highest expression levels detected in leaves. Quantitative real-time PCR analysis showed that the PtrSNAT1 gene was notably upregulated under various stress conditions, suggesting its role in stress response. Overall, these findings provide critical insights for further functional studies of citrus SNAT genes in relation to abiotic stress responses. Moreover, the PtrSNAT1 gene represents a potential target for developing rootstocks with enhanced resistance to abiotic stress.
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Claims 13
Claim · model-written summary
The study identified 21 members of the SNAT gene family across five citrus genomes.
CitrusOrganisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“This study identified 21 members of the SNAT gene family across five citrus genomes”
t0“Citrus”
Claim · model-written summary
The citrus SNAT genes were distributed over five chromosomes, with the majority predicted to localize within chloroplasts.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“distributed over five chromosomes, with the majority predicted to localize within chloroplasts”
t0“Citrus”
Claim · model-written summary
Citrus SNAT genes had between 1 and 8 exons.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“between 1 and 8 exons”
t0“Citrus”
Claim · model-written summary
Citrus SNAT genes had between 0 and 7 introns.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“0 and 7 introns”
t0“Citrus”
Claim · model-written summary
Citrus SNAT genes had 1 to 2 conserved domains.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“1 and 2 conserved domains”
t0“Citrus”
Claim · model-written summary
Citrus SNAT genes had 5 to 8 motifs.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“5 and 8 motifs”
t0“Citrus”
Claim · model-written summary
Phylogenetic analysis classified the citrus SNAT genes into four subgroups.
- Outcome
- classified the genes into four subgroupse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Phylogenetic analysis classified the genes into four subgroups”
t0“Citrus”
Claim · model-written summary
Citrus SNAT genes showed significant collinearity with SNAT genes in rice.
- Outcome
- significant collinearity with SNAT genes in ricee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“demonstrating significant collinearity with SNAT genes in rice”
t0“Citrus”
Claim · model-written summary
Analysis of the SNAT promoter regions revealed 32 cis-acting elements.
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Analysis of the promoter regions revealed 32 cis-acting elements”
t0“Citrus”
Claim · model-written summary
Among the promoter cis-acting elements, those responsive to light, abscisic acid and drought were the most common.
- Outcome
- those responsive to light, abscisic acid, and drought being the most commone0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“those responsive to light, abscisic acid, and drought being the most common”
t0“Citrus”
Claim · model-written summary
SNAT genes in trifoliate orange showed tissue specificity, with the highest expression levels in leaves.
- Taxon
- trifoliate orange
Citrus trifoliatae0 - Organ / stage
- leavese0
- Gene
- SNATe0
- Outcome
- highest expression levelse0
- Direction
- higheste0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Expression analysis of SNAT genes in trifoliate orange indicated tissue specificity, with the highest expression levels detected in leaves”
t0“Citrus”
Claim · model-written summary
PtrSNAT1 was notably upregulated under various stress conditions.
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Quantitative real-time PCR analysis showed that the PtrSNAT1 gene was notably upregulated under various stress conditions”
t0“Citrus”
Claim · model-written summary
The authors suggest PtrSNAT1 is a potential target for developing rootstocks with enhanced resistance to abiotic stress.
- Outcome
- potential target for developing rootstocks with enhanced resistance to abiotic stresse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the PtrSNAT1 gene represents a potential target for developing rootstocks with enhanced resistance to abiotic stress”
t0“Citrus”