Abstract
Verticillium dahliae isolates causing devastating vascular wilt in cotton plants can be divided into defoliating and nondefoliating pathotypes. The mechanisms underlying how V. dahliae uses secretory proteins to manipulate plant physiological processes and suppress immunity in cotton plants have received renewed research focus over the past several years. Here, we describe the role of a secretory protein named VdSAP (secretory aspartyl protease) from the defoliating V. dahliae strain XJ592 in virulence and defoliation. Deletion of VdSAP in strain XJ592 led to significantly reduced virulence and cotton plant defoliation. VdSAP functioned as a protease that targeted cotton plant GhARP (auxin-repressed protein), and VdSAP negatively regulated GhARP content in plants. Transient expression of GhARP in Nicotiana benthamiana enhanced the expression of plant defence signals and inhibited plant abscission signals. Furthermore, GhARP negatively regulated the ethylene (ET) signal and positively regulated the salicylic acid (SA) signal. In addition, GhARP interacted with 1-aminocyclopropane carboxylate oxidases (GhACOs), suggesting that it might function through the ET signal during V. dahliae-cotton plant interactions. These results suggest that GhARP is a molecular link between plant defence and abscission signals, and that VdSAP decreases accumulation of GhARP and enhances the virulence and defoliation caused by V. dahliae.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by-nc. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 10
Claim · model-written summary
Deletion of VdSAP significantly reduced Verticillium dahliae virulence on cotton.
- Taxon
- cotton
Gossypium hirsutume0 - Treatment
- Deletion of VdSAPe1
- Interactor
- Verticillium dahliae
Verticillium dahliaee0pathogen - Outcome
- virulencee1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Verticillium dahliae isolates causing devastating vascular wilt in cotton plants”
e1“Deletion of VdSAP in strain XJ592 led to significantly reduced virulence and cotton plant defoliation”
Claim · model-written summary
Deletion of VdSAP significantly reduced cotton plant defoliation caused by Verticillium dahliae.
- Taxon
- cottonGossypium hirsutume0
- Treatment
- Deletion of VdSAPe1
- Interactor
- Verticillium dahliaeVerticillium dahliaee0pathogen
- Outcome
- cotton plant defoliatione1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Verticillium dahliae isolates causing devastating vascular wilt in cotton plants”
e1“Deletion of VdSAP in strain XJ592 led to significantly reduced virulence and cotton plant defoliation”
Claim · model-written summary
VdSAP functions as a protease that targets cotton GhARP.
- Taxon
- cottonGossypium hirsutume0
- Gene
- GhARPe2
- Outcome
- targeted cotton plant GhARPe2
- Qualifier
- functioned as a proteasee2
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Verticillium dahliae isolates causing devastating vascular wilt in cotton plants”
e2“VdSAP functioned as a protease that targeted cotton plant GhARP”
Claim · model-written summary
VdSAP negatively regulates GhARP content in cotton plants.
- Taxon
- cottonGossypium hirsutume0
- Gene
- VdSAPe3
- Outcome
- GhARP contente3
- Direction
- negatively regulatede3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Verticillium dahliae isolates causing devastating vascular wilt in cotton plants”
e3“VdSAP negatively regulated GhARP content in plants”
Claim · model-written summary
Transient expression of GhARP in Nicotiana benthamiana enhanced expression of plant defence signals.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae4 - Treatment
- Transient expression of GhARPe4
- Gene
- GhARPe4
- Outcome
- expression of plant defence signalse4
- Direction
- enhancede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Transient expression of GhARP in Nicotiana benthamiana enhanced the expression of plant defence signals and inhibited plant abscission signals”
Claim · model-written summary
Transient expression of GhARP in Nicotiana benthamiana inhibited plant abscission signals.
- Taxon
- Nicotiana benthamianaNicotiana benthamianae4
- Treatment
- Transient expression of GhARPe4
- Gene
- GhARPe4
- Outcome
- plant abscission signalse4
- Direction
- inhibitede4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e4“Transient expression of GhARP in Nicotiana benthamiana enhanced the expression of plant defence signals and inhibited plant abscission signals”
Claim · model-written summary
GhARP negatively regulates the ethylene signal in cotton.
- Taxon
- cottonGossypium hirsutume0
- Gene
- GhARPe5
- Outcome
- ethylene (ET) signale5
- Direction
- negatively regulatede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Verticillium dahliae isolates causing devastating vascular wilt in cotton plants”
e5“GhARP negatively regulated the ethylene (ET) signal and positively regulated the salicylic acid (SA) signal”
Claim · model-written summary
GhARP positively regulates the salicylic acid signal in cotton.
- Taxon
- cottonGossypium hirsutume0
- Gene
- GhARPe5
- Outcome
- salicylic acid (SA) signale5
- Direction
- positively regulatede5
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Verticillium dahliae isolates causing devastating vascular wilt in cotton plants”
e5“GhARP negatively regulated the ethylene (ET) signal and positively regulated the salicylic acid (SA) signal”
Claim · model-written summary
GhARP interacted with 1-aminocyclopropane carboxylate oxidases (GhACOs) in cotton.
- Taxon
- cottonGossypium hirsutume0
- Gene
- GhARPe6
- Outcome
- interacted with 1-aminocyclopropane carboxylate oxidases (GhACOs)e6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Verticillium dahliae isolates causing devastating vascular wilt in cotton plants”
e6“GhARP interacted with 1-aminocyclopropane carboxylate oxidases (GhACOs)”
Claim · model-written summary
GhARP may function through the ethylene signal during Verticillium dahliae-cotton interactions.
- Taxon
- cottonGossypium hirsutume0
- Interactor
- V. dahliaeVerticillium dahliaee7pathogen
- Outcome
- function through the ET signale7
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Verticillium dahliae isolates causing devastating vascular wilt in cotton plants”
e7“suggesting that it might function through the ET signal during V. dahliae-cotton plant interactions”
The same questions in other papers
transgenic · molecular: expression · Nicotiana benthamianaPositive and negative results
19 papers in the same direction as this paper · 9 in the opposite direction
- negative
- negative
- no effect
25 papers more in this group
See all 29 papers →loss of function · Verticillium dahliae · Gossypium hirsutum (cotton)Positive and negative results
33 papers in the same direction as this paper · 59 in the opposite direction
- negative
- positive
- positive
89 papers more in this group
See all 93 papers →transgenic · yield · Nicotiana benthamianaResults agree
1 paper in the same direction as this paper
- positive
Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.