Abstract
Spike length (SL) is an important factor affecting yield in wheat ( Triticum aestivum L.). Here, a recombinant inbred line (RIL) population derived from a cross between Shannong 4155 (SN4155) and Shimai 12 (SM12) was used to map quantitative trait loci (QTL) controlling SL. A QTL, qSL2B , on chromosome 2B was identified in all experiments and explained 9.92%–12.71% of the phenotypic variation. Through transcriptome and gene expression analysis, we identified a gene encoding Elongation Factor 1-alpha ( TaeEF1A ) as the candidate gene for qSL2B . Genome editing of TaeEF1A demonstrated that TaeEF1A positively regulates SL, spikelet number per spike (SNS), and grain number per spike (GN). Transcriptome analysis showed that TaeEF1A may affect the protein translation process and photosynthesis to regulate spike development. We used haplotype analysis of wheat germplasm to identify seven types of genetic variations in TaeEF1A , with Type I, Type II, and Type III being the major haplotypes. Screening of 428 cultivars and breeding lines identified 225 and 203 accessions as Type I and Type II haplotypes, respectively, with Type III not detected. Comparison of SL, SNS, and GN between the Type I and Type II haplotypes revealed that the Type I allele can increase SL, SNS, and GN simultaneously, and is thus preferred for use in wheat molecular breeding efforts to increase SL, SNS, and GN.
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Claims 10
Claim · model-written summary
In wheat, the QTL qSL2B on chromosome 2B was identified in all experiments and explained 9.92%-12.71% of the phenotypic variation in spike length.
- Taxon
- wheat
Triticum aestivumt0 - Gene
- qSL2Be0
- Outcome
- phenotypic variatione0
- Value
- 9.92%–12.71%e0
Not reportedDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“A QTL, qSL2B , on chromosome 2B was identified in all experiments and explained 9.92%–12.71% of the phenotypic variation”
t0“wheat”
Claim · model-written summary
TaeEF1A, encoding Elongation Factor 1-alpha, was identified as the candidate gene for qSL2B by transcriptome and gene expression analysis in wheat.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaeEF1Ae0
- Outcome
- candidate gene for qSL2Be0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Through transcriptome and gene expression analysis, we identified a gene encoding Elongation Factor 1-alpha ( TaeEF1A ) as the candidate gene for qSL2B”
t0“wheat”
Claim · model-written summary
Genome editing of TaeEF1A showed that TaeEF1A positively regulates spike length, spikelet number per spike and grain number per spike in wheat.
- Taxon
- wheatTriticum aestivumt0
- Treatment
- Genome editing of TaeEF1Ae0
- Gene
- TaeEF1Ae0
- Outcome
- SL, spikelet number per spike (SNS), and grain number per spike (GN)e0
- Direction
- positively regulatese0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Genome editing of TaeEF1A demonstrated that TaeEF1A positively regulates SL, spikelet number per spike (SNS), and grain number per spike (GN)”
t0“wheat”
Claim · model-written summary
Transcriptome analysis indicated that TaeEF1A may affect the protein translation process and photosynthesis to regulate spike development in wheat.
- Taxon
- wheatTriticum aestivumt0
- Gene
- TaeEF1Ae0
- Outcome
- spike developmente0
- Qualifier
- protein translation process and photosynthesise0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transcriptome analysis showed that TaeEF1A may affect the protein translation process and photosynthesis to regulate spike development”
t0“wheat”
Claim · model-written summary
Haplotype analysis of wheat germplasm identified seven types of genetic variations in TaeEF1A.
- Taxon
- wheatTriticum aestivume0
- Gene
- TaeEF1Ae0
- Outcome
- seven types of genetic variationse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“We used haplotype analysis of wheat germplasm to identify seven types of genetic variations in TaeEF1A”
Claim · model-written summary
Among 428 wheat cultivars and breeding lines screened, 225 accessions were Type I TaeEF1A haplotype.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- accessions as Type Ie0
- Value
- 225e0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Screening of 428 cultivars and breeding lines identified 225 and 203 accessions as Type I and Type II haplotypes, respectively, with Type III not detected”
t0“wheat”
Claim · model-written summary
Among 428 wheat cultivars and breeding lines screened, 203 accessions were Type II TaeEF1A haplotype.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- Type II haplotypese0
- Value
- 203e0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Screening of 428 cultivars and breeding lines identified 225 and 203 accessions as Type I and Type II haplotypes, respectively, with Type III not detected”
t0“wheat”
Claim · model-written summary
The Type III TaeEF1A haplotype was not detected among the 428 wheat cultivars and breeding lines screened.
- Taxon
- wheatTriticum aestivumt0
- Outcome
- Type III not detectede0
Not reportedGene
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Screening of 428 cultivars and breeding lines identified 225 and 203 accessions as Type I and Type II haplotypes, respectively, with Type III not detected”
t0“wheat”
Claim · model-written summary
In wheat, the TaeEF1A Type I allele increased spike length, spikelet number per spike and grain number per spike simultaneously compared with the Type II haplotype.
- Taxon
- wheatTriticum aestivumt0
- Material
- Type I allelee0
- Outcome
- SL, SNS, and GNe0
- Direction
- increasee0
- Comparator
- Type II haplotypese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Comparison of SL, SNS, and GN between the Type I and Type II haplotypes revealed that the Type I allele can increase SL, SNS, and GN simultaneously”
t0“wheat”
Claim · model-written summary
The authors recommend the Type I allele for use in wheat molecular breeding to increase spike length, spikelet number and grain number per spike.
- Taxon
- wheatTriticum aestivume0
- Outcome
- preferred for use in wheat molecular breeding effortse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“is thus preferred for use in wheat molecular breeding efforts to increase SL, SNS, and GN”
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