Abstract
Plant fungal diseases remain a major threat to global agricultural production, necessitating eco-friendly and sustainable strategies. Conventional chemical fungicides often lead to the development of resistant pathogen strains and cause environmental contamination. Therefore, the development of biocontrol agents is particularly important. In this study, we identified Burkholderia stagnalis YJ-2 from the rhizosphere soil of Woodsia ilvensis as a promising biocontrol strain using 16S rRNA and whole-genome sequencing. This strain demonstrated broad-spectrum antifungal activity against plant fungal pathogens, with its bioactive extracts maintaining high stability across a temperature range of 25–100 °C and pH range of 2–12. We used in vitro assays to further show that the metabolites of B. stagnalis YJ-2 disrupted the hyphal morphology of Valsa mali, resulting in swelling, reduced branching, and increased pigmentation. Fluorescence labeling confirmed that B. stagnalis YJ-2 stably colonized the roots and stems of tomato and wheat plants. Furthermore, various formulations of microbial agents based on B. stagnalis YJ-2 were evaluated for their efficacy against plant pathogens. The seed-coating formulation notably protected tomato seedlings from Alternaria solani infection without affecting germination (p > 0.1), while the wettable powder exhibited significant control effects on early blight in tomatoes, with the preventive treatment showing better efficacy than the therapeutic treatment. Additionally, the B. stagnalis YJ-2 bone glue agent showed a substantial inhibitory effect on apple tree canker. Whole-genome analysis of B. stagnalis YJ-2 revealed a 7,705,355 bp genome (67.68% GC content) with 6858 coding genes and 20 secondary metabolite clusters, including three clusters (YJ-2_GM002015-YJ-2_GM002048, YJ-2_GM0020090-YJ-2_GM002133, and YJ-2_GM06534-YJ-2_GM006569) that are related to the antifungal activity of YJ-2 and are homologous to the biosynthetic gene clusters of known secondary metabolites, such as icosalide, ornibactin, and sinapigladioside. We further knocked out core biosynthetic genes of two secondary metabolic gene clusters and found that only the YJ-2_GM006534-YJ-2_GM006569 gene cluster had a corresponding function in two potential antifungal gene clusters. In contrast to the wild-type strain YJ-2, only deletion of the YJ-2_GM006563 gene reduced the antifungal activity of B. stagnalis YJ-2 by 8.79%. These findings highlight the biocontrol potential of B. stagnalis YJ-2, supporting a theoretical foundation for its development as a biocontrol agent against plant fungal diseases and thereby promoting sustainable agricultural disease management.
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Claims 7
Claim · model-written summary
B. stagnalis YJ-2 stably colonized tomato roots and stems.
- Taxon
- tomato
Solanum lycopersicume0 - Interactor
- B. stagnalis YJ-2Burkholderia stagnalise0biocontrol_agente1
- Outcome
- stably colonized the roots and stemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fluorescence labeling confirmed that B. stagnalis YJ-2 stably colonized the roots and stems of tomato and wheat plants”
e1“In this study, we identified Burkholderia stagnalis YJ-2 from the rhizosphere soil of Woodsia ilvensis as a promising biocontrol strain”
Claim · model-written summary
B. stagnalis YJ-2 stably colonized wheat roots and stems.
- Taxon
- wheat
Triticum aestivume0 - Interactor
- B. stagnalis YJ-2Burkholderia stagnalise0biocontrol_agente1
- Outcome
- stably colonized the roots and stemse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Fluorescence labeling confirmed that B. stagnalis YJ-2 stably colonized the roots and stems of tomato and wheat plants”
e1“In this study, we identified Burkholderia stagnalis YJ-2 from the rhizosphere soil of Woodsia ilvensis as a promising biocontrol strain”
Claim · model-written summary
YJ-2 seed coating protected tomato seedlings from Alternaria solani infection.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- seed-coating formulatione0
- Interactor
- Alternaria solani
Alternaria solanie0pathogen - Second interactor
- Burkholderia stagnalis YJ-2Burkholderia stagnalise1biocontrol_agent
- Outcome
- Alternaria solani infectione0
- Direction
- protectede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The seed-coating formulation notably protected tomato seedlings from Alternaria solani infection without affecting germination (p > 0.1)”
e1“In this study, we identified Burkholderia stagnalis YJ-2 from the rhizosphere soil of Woodsia ilvensis as a promising biocontrol strain”
Claim · model-written summary
YJ-2 seed coating did not significantly change tomato germination.
- Taxon
- tomatoSolanum lycopersicume0
- Treatment
- seed-coating formulatione0
- Interactor
- Burkholderia stagnalis YJ-2Burkholderia stagnalise1biocontrol_agent
- Outcome
- germinatione0
- Direction
- without affectinge0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“The seed-coating formulation notably protected tomato seedlings from Alternaria solani infection without affecting germination (p > 0.1)”
e1“In this study, we identified Burkholderia stagnalis YJ-2 from the rhizosphere soil of Woodsia ilvensis as a promising biocontrol strain”
Claim · model-written summary
YJ-2 wettable powder significantly controlled early blight in tomatoes.
- Taxon
- tomatoesSolanum lycopersicume0
- Treatment
- wettable powdere0
- Interactor
- Burkholderia stagnalis YJ-2Burkholderia stagnalise1biocontrol_agent
- Outcome
- early blighte0
- Direction
- control effectse0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the wettable powder exhibited significant control effects on early blight in tomatoes, with the preventive treatment showing better efficacy than the therapeutic treatment”
e1“In this study, we identified Burkholderia stagnalis YJ-2 from the rhizosphere soil of Woodsia ilvensis as a promising biocontrol strain”
Claim · model-written summary
Preventive wettable powder treatment controlled tomato early blight better than therapeutic treatment.
- Taxon
- tomatoesSolanum lycopersicume0
- Treatment
- preventive treatmente0
- Interactor
- Burkholderia stagnalis YJ-2Burkholderia stagnalise1biocontrol_agent
- Outcome
- efficacye0
- Direction
- bettere0
- Comparator
- therapeutic treatmente0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the wettable powder exhibited significant control effects on early blight in tomatoes, with the preventive treatment showing better efficacy than the therapeutic treatment”
e1“In this study, we identified Burkholderia stagnalis YJ-2 from the rhizosphere soil of Woodsia ilvensis as a promising biocontrol strain”
Claim · model-written summary
YJ-2 bone glue agent substantially inhibited apple tree canker.
- Taxon
- apple tree
Malus domesticae0 - Treatment
- B. stagnalis YJ-2 bone glue agente0
- Interactor
- B. stagnalis YJ-2Burkholderia stagnalise0biocontrol_agente1
- Outcome
- apple tree cankere0
- Direction
- inhibitorye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the B. stagnalis YJ-2 bone glue agent showed a substantial inhibitory effect on apple tree canker”
e1“In this study, we identified Burkholderia stagnalis YJ-2 from the rhizosphere soil of Woodsia ilvensis as a promising biocontrol strain”
The same questions in other papers
seed treatment · Alternaria solani · Solanum lycopersicum (tomato)Results agree
6 papers in the same direction as this paper
- positive
- positive
- positive
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See all 7 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.