Abstract
Cellulose is synthesized by cellulose synthases (CESAs) in plasma membrane-localized complexes, which act as a central component of the cell wall and influence plant growth and defense responses. Puccinia striiformis f. sp. tritici (Pst) is an airborne fungus that causes stripe rust to seriously endanger wheat production. In this study, a CESA gene, TaCESA7, was identified to be significantly up-regulated during Pst infection in wheat (Triticum aestivum L.). TaCESA7 was localized on the plasma membrane in dimeric form, and the dimers interact to assemble into CESA complexes. Stable overexpression of TaCESA7 weakened the resistance of wheat to Pst. Knockdown of TaCESA7 by RNA interference (RNAi) and virus-induced gene silencing led to restricted hyphal spread, increased necrotic area, and simultaneously promotes reactive oxygen species (ROS) accumulation and the expression of pathogenesis-related (PR) genes. Transcriptome analysis of TaCESA7-RNAi plants revealed that the up-regulated genes were significantly enriched in the phenylpropanoid biosynthesis and plant-pathogen interaction pathways. Moreover, silencing TaCESA7 promoted the deposition of lignin and the expression of genes related to lignin synthesis. CRISPR-Cas9-mediated inactivation of TaCESA7 in wheat could confer broad-spectrum resistance against Pst without affecting agronomic traits. These findings provide valuable candidate gene resources and guidance for molecular breeding to improve the resistance of wheat to fungal disease.
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Claims 8
Claim · model-written summary
TaCESA7 was significantly up-regulated during Pst infection in wheat.
- Taxon
- wheat
Triticum aestivume1 - Interactor
- Puccinia striiformis f. sp. tritici
Puccinia striiformis f. sp. triticie0pathogen - Gene
- TaCESA7e1
- Outcome
- significantly up-regulatede1
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Puccinia striiformis f. sp. tritici (Pst) is an airborne fungus that causes stripe rust”
e1“a CESA gene, TaCESA7, was identified to be significantly up-regulated during Pst infection in wheat (Triticum aestivum L.)”
Claim · model-written summary
Stable overexpression of TaCESA7 weakened the resistance of wheat to Pst.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Stable overexpression of TaCESA7e2
- Interactor
- Pste2pathogen
- Gene
- TaCESA7e2
- Outcome
- resistance of wheat to Pste2
- Direction
- weakenede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a CESA gene, TaCESA7, was identified to be significantly up-regulated during Pst infection in wheat (Triticum aestivum L.)”
e2“Stable overexpression of TaCESA7 weakened the resistance of wheat to Pst”
Claim · model-written summary
Knockdown of TaCESA7 restricted hyphal spread of Pst in wheat.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Knockdown of TaCESA7 by RNA interference (RNAi) and virus-induced gene silencinge3
- Interactor
- Pste1pathogen
- Gene
- TaCESA7e3
- Outcome
- hyphal spreade3
- Direction
- restrictede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a CESA gene, TaCESA7, was identified to be significantly up-regulated during Pst infection in wheat (Triticum aestivum L.)”
e3“Knockdown of TaCESA7 by RNA interference (RNAi) and virus-induced gene silencing led to restricted hyphal spread, increased necrotic area, and simultaneously promotes reactive oxygen species (ROS) accumulation and the expression of pathogenesis-related (PR) genes”
Claim · model-written summary
Knockdown of TaCESA7 increased the necrotic area on wheat leaves infected with Pst.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Knockdown of TaCESA7 by RNA interference (RNAi) and virus-induced gene silencinge3
- Gene
- TaCESA7e3
- Outcome
- necrotic areae3
- Direction
- increasede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a CESA gene, TaCESA7, was identified to be significantly up-regulated during Pst infection in wheat (Triticum aestivum L.)”
e3“Knockdown of TaCESA7 by RNA interference (RNAi) and virus-induced gene silencing led to restricted hyphal spread, increased necrotic area, and simultaneously promotes reactive oxygen species (ROS) accumulation and the expression of pathogenesis-related (PR) genes”
Claim · model-written summary
Knockdown of TaCESA7 promoted reactive oxygen species accumulation and the expression of pathogenesis-related genes.
- Taxon
- wheatTriticum aestivume1
- Treatment
- Knockdown of TaCESA7 by RNA interference (RNAi) and virus-induced gene silencinge3
- Gene
- TaCESA7e3
- Outcome
- reactive oxygen species (ROS) accumulation and the expression of pathogenesis-related (PR) genese3
- Direction
- promotese3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a CESA gene, TaCESA7, was identified to be significantly up-regulated during Pst infection in wheat (Triticum aestivum L.)”
e3“Knockdown of TaCESA7 by RNA interference (RNAi) and virus-induced gene silencing led to restricted hyphal spread, increased necrotic area, and simultaneously promotes reactive oxygen species (ROS) accumulation and the expression of pathogenesis-related (PR) genes”
Claim · model-written summary
Up-regulated genes in TaCESA7-RNAi wheat plants were significantly enriched in the phenylpropanoid biosynthesis and plant-pathogen interaction pathways.
- Taxon
- wheatTriticum aestivume1
- Gene
- TaCESA7-RNAie4
- Outcome
- enriched in the phenylpropanoid biosynthesis and plant-pathogen interaction pathwayse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a CESA gene, TaCESA7, was identified to be significantly up-regulated during Pst infection in wheat (Triticum aestivum L.)”
e4“Transcriptome analysis of TaCESA7-RNAi plants revealed that the up-regulated genes were significantly enriched in the phenylpropanoid biosynthesis and plant-pathogen interaction pathways”
Claim · model-written summary
Silencing TaCESA7 promoted lignin deposition and the expression of lignin-synthesis genes in wheat.
- Taxon
- wheatTriticum aestivume1
- Treatment
- silencing TaCESA7e5
- Gene
- TaCESA7e5
- Outcome
- deposition of lignin and the expression of genes related to lignin synthesise5
- Direction
- promotede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a CESA gene, TaCESA7, was identified to be significantly up-regulated during Pst infection in wheat (Triticum aestivum L.)”
e5“silencing TaCESA7 promoted the deposition of lignin and the expression of genes related to lignin synthesis”
Claim · model-written summary
CRISPR-Cas9-mediated inactivation of TaCESA7 could confer broad-spectrum resistance against Pst in wheat without affecting agronomic traits.
- Taxon
- wheatTriticum aestivume1
- Treatment
- CRISPR-Cas9-mediated inactivation of TaCESA7e6
- Gene
- TaCESA7e6
- Outcome
- broad-spectrum resistance against Pste6
- Direction
- confere6
- Qualifier
- without affecting agronomic traitse6
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e1“a CESA gene, TaCESA7, was identified to be significantly up-regulated during Pst infection in wheat (Triticum aestivum L.)”
e6“CRISPR-Cas9-mediated inactivation of TaCESA7 in wheat could confer broad-spectrum resistance against Pst without affecting agronomic traits”
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See all 177 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.