Paper · 2025 · Applied Sciences

Application of Difenoconazole and Trichoderma Broth Combination for Synergistic Control of Corn Leaf Blight and Stalk Rot in Straw-Returned Fields in Liaoning Province, China

Ping Wang; Lijuan Wang; Kejie Liu; et al.

7
claims
18
verbatim quotes
2
citations
2
research areas
✓
Open access · cc-by

Abstract

Maize production in Fuxin City, Liaoning Province, China, is threatened by northern corn leaf blight (NCLB) and Fusarium stalk rot, with straw return under conservation tillage exacerbating the NCLB severity by 20% in local fields. This study evaluated the efficacy of combining difenoconazole, a commonly used fungicide, with a Trichoderma bioagent for disease control in straw-incorporated soils. Field trials in Fuxin showed that applying 300 g/ha difenoconazole with 1.5 L/ha Trichoderma fermentate achieved superior results: a 72.4% reduction in the NCLB disease index and a stalk rot incidence of only 0.61%. These outcomes significantly outperformed single-component treatments like difenoconazole alone (56.2% NCLB suppression) or other fungicides (e.g., carbendazim, triadimefon). The combined treatment also outperformed the single treatments with biocontrol agent (67.1% NCLB inhibition). The results highlight the synergistic potential of integrating chemical and biological agents to manage residue-borne diseases, offering a practical strategy for sustainable disease control in conservation agriculture systems with straw return in Liaoning, China.

Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗

The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.

Claims 7

Associationobservedoriginal resultnegativemeasured

Claim · model-written summary

Straw return exacerbated northern corn leaf blight severity by 20% in local maize fields.

Taxon
MaizeZea mayse1
Treatment
straw return under conservation tillagee0
Interactor
northern corn leaf blightExserohilum turcicume1pathogen
Outcome
NCLB severitye0
Direction
exacerbatinge0
Value
20%e0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Exserohilum turcicum
Exserohilum turcicum · InteractorWanich Khampanich, Department of Agriculture, Thailand · CC BY 3.0 au · Wikimedia Commons ↗

Verbatim

e0

“straw return under conservation tillage exacerbating the NCLB severity by 20% in local fields”

✓ located verbatim in the source · abstract, characters 137–230 · show in text ↑
e1

“Maize production in Fuxin City, Liaoning Province, China, is threatened by northern corn leaf blight (NCLB)”

✓ located verbatim in the source · abstract, characters 0–107 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Difenoconazole plus Trichoderma fermentate reduced NCLB disease index by 72.4%.

Taxon
MaizeZea mayse1
Treatment
300 g/ha difenoconazole with 1.5 L/ha Trichoderma fermentatee0
Interactor
northern corn leaf blightExserohilum turcicume1pathogen
Second interactor
TrichodermaTrichodermae0biocontrol_agent
Outcome
NCLB disease indexe0
Direction
reductione0
Value
72.4%e0
Comparator
single-component treatmentse2

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Exserohilum turcicum
Exserohilum turcicum · InteractorWanich Khampanich, Department of Agriculture, Thailand · CC BY 3.0 au · Wikimedia Commons ↗
Trichoderma
Trichoderma · Second interactor · picture of the genus (one of its species)US Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“applying 300 g/ha difenoconazole with 1.5 L/ha Trichoderma fermentate achieved superior results: a 72.4% reduction in the NCLB disease index”

✓ located verbatim in the source · abstract, characters 433–573 · show in text ↑
e1

“Maize production in Fuxin City, Liaoning Province, China, is threatened by northern corn leaf blight (NCLB)”

✓ located verbatim in the source · abstract, characters 0–107 · show in text ↑
e2

“single-component treatments like difenoconazole alone”

✓ located verbatim in the source · abstract, characters 657–710 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The combined treatment yielded a stalk rot incidence of 0.61%.

Taxon
MaizeZea mayse1
Treatment
300 g/ha difenoconazole with 1.5 L/ha Trichoderma fermentatee2
Interactor
FusariumFusariume1pathogen
Second interactor
TrichodermaTrichodermae2biocontrol_agent
Outcome
stalk rot incidencee0
Direction
onlye0
Value
0.61%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Fusarium
Fusarium · Interactor · picture of the genus (one of its species)Public domain · Wikimedia Commons ↗
Trichoderma
Trichoderma · Second interactor · picture of the genus (one of its species)US Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“a stalk rot incidence of only 0.61%”

✓ located verbatim in the source · abstract, characters 578–613 · show in text ↑
e1

“Maize production in Fuxin City, Liaoning Province, China, is threatened by northern corn leaf blight (NCLB) and Fusarium stalk rot”

✓ located verbatim in the source · abstract, characters 0–130 · show in text ↑
e2

“applying 300 g/ha difenoconazole with 1.5 L/ha Trichoderma fermentate”

✓ located verbatim in the source · abstract, characters 433–502 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Difenoconazole alone suppressed NCLB by 56.2%.

Taxon
MaizeZea mayse1
Treatment
difenoconazole alonee0
Interactor
northern corn leaf blightExserohilum turcicume1pathogen
Outcome
NCLB suppressione0
Direction
suppressione0
Value
56.2%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Exserohilum turcicum
Exserohilum turcicum · InteractorWanich Khampanich, Department of Agriculture, Thailand · CC BY 3.0 au · Wikimedia Commons ↗

Verbatim

e0

“difenoconazole alone (56.2% NCLB suppression)”

✓ located verbatim in the source · abstract, characters 690–735 · show in text ↑
e1

“Maize production in Fuxin City, Liaoning Province, China, is threatened by northern corn leaf blight (NCLB)”

✓ located verbatim in the source · abstract, characters 0–107 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

The single biocontrol agent treatment inhibited NCLB by 67.1%.

Taxon
MaizeZea mayse1
Treatment
single treatments with biocontrol agente0
Interactor
northern corn leaf blightExserohilum turcicume1pathogen
Second interactor
TrichodermaTrichodermae2biocontrol_agent
Outcome
NCLB inhibitione0
Direction
inhibitione0
Value
67.1%e0

Not reportedComparator

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Exserohilum turcicum
Exserohilum turcicum · InteractorWanich Khampanich, Department of Agriculture, Thailand · CC BY 3.0 au · Wikimedia Commons ↗
Trichoderma
Trichoderma · Second interactor · picture of the genus (one of its species)US Department of Agriculture, Agricultural Research Service, Systematic Botany and Mycology Laboratory, [1] · Public domain · Wikimedia Commons ↗

Verbatim

e0

“single treatments with biocontrol agent (67.1% NCLB inhibition)”

✓ located verbatim in the source · abstract, characters 835–898 · show in text ↑
e1

“Maize production in Fuxin City, Liaoning Province, China, is threatened by northern corn leaf blight (NCLB)”

✓ located verbatim in the source · abstract, characters 0–107 · show in text ↑
e2

“with a Trichoderma bioagent”

✓ located verbatim in the source · abstract, characters 322–349 · show in text ↑
Intervention effectobservedoriginal resultpositivesignificant (as stated)measured

Claim · model-written summary

The combination significantly outperformed single components and other fungicides for disease control.

Taxon
MaizeZea mayse1
Treatment
combining difenoconazolee2
Interactor
northern corn leaf blightExserohilum turcicume1pathogen
Outcome
outcomese0
Direction
outperformede0
Comparator
single-component treatmentse0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗
Exserohilum turcicum
Exserohilum turcicum · InteractorWanich Khampanich, Department of Agriculture, Thailand · CC BY 3.0 au · Wikimedia Commons ↗

Verbatim

e0

“These outcomes significantly outperformed single-component treatments like difenoconazole alone (56.2% NCLB suppression) or other fungicides (e.g., carbendazim, triadimefon)”

✓ located verbatim in the source · abstract, characters 615–788 · show in text ↑
e1

“Maize production in Fuxin City, Liaoning Province, China, is threatened by northern corn leaf blight (NCLB)”

✓ located verbatim in the source · abstract, characters 0–107 · show in text ↑
e2

“combining difenoconazole, a commonly used fungicide, with a Trichoderma bioagent”

✓ located verbatim in the source · abstract, characters 269–349 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

The authors propose combining chemical and biological agents for residue-borne disease control.

Taxon
MaizeZea mayse1
Outcome
integrating chemical and biological agents to manage residue-borne diseasese0

Organisms pictured · Representative pictures of the matched species, not figures from the paper.

Zea mays
Zea mays · TaxonAkwugo · CC BY-SA 4.0 · Wikimedia Commons ↗

Verbatim

e0

“integrating chemical and biological agents to manage residue-borne diseases, offering a practical strategy for sustainable disease control in conservation agriculture systems with straw return in Liaoning, China”

✓ located verbatim in the source · abstract, characters 951–1162 · show in text ↑
e1

“Maize production in Fuxin City, Liaoning Province, China”

✓ located verbatim in the source · abstract, characters 0–56 · show in text ↑

difenoconazole · Fusarium · Zea mays (maize)Results agree

2 papers: 2 positive, 0 negative or no effect

1 paper in the same direction as this paper

  1. positive
See all 2 papers →

difenoconazole · Exserohilum turcicum · Zea mays (maize)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

biocontrol · Exserohilum turcicum · Zea mays (maize)Results agree

4 papers: 4 positive, 0 negative or no effect

3 papers in the same direction as this paper

  1. positive
  2. positive
  3. positive
See all 4 papers →

Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.