Abstract
TOPLESS (TPL) and TOPLESS-Related (TPR) corepressors are key regulatory proteins that interact with a variety of transcription factors to form specific complexes, thereby modulating a wide range of signaling pathways and metabolic processes. This study explored the function of the rice TPR gene OsTPR1. Transgenic rice lines overexpressing OsTPR1 (OsTPR1-Ox) exhibited reduced lateral root density, whereas OsTPR1 RNA interference lines (OsTPR1-Ri) showed increased lateral root density. To gain further insight, these transgenic lines were crossed with the DR5::GUS auxin reporter line. In 7-day-old seedlings, lateral root formation occurred in the differentiation zone of seminal roots, with GUS staining prominently localized in the lateral root primordia of the DR5::GUS/OsTPR1-Ri line. Similar results were observed in 45-day-old seedlings, where the DR5::GUS/OsTPR1-Ri line exhibited stronger GUS staining and a higher number of lateral roots in the crown root differentiation zones. In contrast, the DR5::GUS/OsTPR1-Ox line showed weaker GUS signals and fewer lateral roots. Additionally, the expression levels of several auxin efflux transporter genes encoding PIN-FORMED (PIN) proteins, including OsPIN1a, OsPIN1b, OsPIN1c, OsPIN2, and OsPIN5a, were increased in the OsTPR1-Ri line but decreased in the OsTPR1-Ox line. These results suggest that OsTPR1 also modulates the expression of multiple OsPIN genes, thereby potentially influencing auxin responses and lateral root development. Beyond root development, OsTPR1 overexpression led to a significant increase in tiller angle and a delay in flowering time, whereas OsTPR1-Ri plants exhibited earlier flowering. These findings indicate that OsTPR1 acts as a negative regulator of the auxin response, with its overexpression leading to reduced auxin sensitivity and altered plant architecture. Our results show that OsTPR1 modulates lateral root development, tiller angle, and flowering time, contributing to the coordinated growth and development in rice.
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Claims 13
Claim · model-written summary
OsTPR1 overexpression reduced lateral root density.
- Taxon
- rice
Oryza sativat0 - Treatment
- overexpressing OsTPR1e0
- Gene
- OsTPR1e0
- Outcome
- lateral root densitye0
- Direction
- reducede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transgenic rice lines overexpressing OsTPR1 (OsTPR1-Ox) exhibited reduced lateral root density”
t0“rice”
Claim · model-written summary
OsTPR1 RNA interference increased lateral root density.
- Taxon
- riceOryza sativat0
- Treatment
- OsTPR1 RNA interferencee0
- Gene
- OsTPR1e0
- Outcome
- lateral root densitye0
- Direction
- increasede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsTPR1 RNA interference lines (OsTPR1-Ri) showed increased lateral root density”
t0“rice”
Claim · model-written summary
In seven-day seedlings, GUS staining was prominent in lateral root primordia of the RNA interference reporter line.
- Taxon
- riceOryza sativat0
- Material
- DR5::GUS/OsTPR1-Rie0
- Organ / stage
- lateral root primordiae0
- Outcome
- GUS staining prominently localized in the lateral root primordiae0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“In 7-day-old seedlings, lateral root formation occurred in the differentiation zone of seminal roots, with GUS staining prominently localized in the lateral root primordia of the DR5::GUS/OsTPR1-Ri line”
t0“rice”
Claim · model-written summary
At 45 days, the RNA interference reporter line had stronger GUS staining in crown root differentiation zones.
- Taxon
- riceOryza sativat0
- Material
- DR5::GUS/OsTPR1-Rie0
- Organ / stage
- crown root differentiation zonese0
- Outcome
- GUS staininge0
- Direction
- strongere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the DR5::GUS/OsTPR1-Ri line exhibited stronger GUS staining and a higher number of lateral roots in the crown root differentiation zones”
t0“rice”
Claim · model-written summary
At 45 days, the RNA interference reporter line had more lateral roots in crown root differentiation zones.
- Taxon
- riceOryza sativat0
- Material
- DR5::GUS/OsTPR1-Rie0
- Outcome
- number of lateral rootse0
- Direction
- highere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the DR5::GUS/OsTPR1-Ri line exhibited stronger GUS staining and a higher number of lateral roots in the crown root differentiation zones”
t0“rice”
Claim · model-written summary
The OsTPR1 overexpression reporter line showed weaker GUS signals.
- Taxon
- riceOryza sativat0
- Material
- DR5::GUS/OsTPR1-Oxe0
- Outcome
- GUS signalse0
- Direction
- weakere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the DR5::GUS/OsTPR1-Ox line showed weaker GUS signals and fewer lateral roots”
t0“rice”
Claim · model-written summary
The OsTPR1 overexpression reporter line showed fewer lateral roots.
- Taxon
- riceOryza sativat0
- Material
- DR5::GUS/OsTPR1-Oxe0
- Outcome
- lateral rootse0
- Direction
- fewere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the DR5::GUS/OsTPR1-Ox line showed weaker GUS signals and fewer lateral roots”
t0“rice”
Claim · model-written summary
The RNA interference line increased expression of five named OsPIN genes.
- Taxon
- riceOryza sativat0
- Material
- OsTPR1-Rie0
- Gene
- OsPIN1a, OsPIN1b, OsPIN1c, OsPIN2, and OsPIN5ae0
- Outcome
- expression levelse0
- Direction
- increasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression levels of several auxin efflux transporter genes encoding PIN-FORMED (PIN) proteins, including OsPIN1a, OsPIN1b, OsPIN1c, OsPIN2, and OsPIN5a, were increased in the OsTPR1-Ri line”
t0“rice”
Claim · model-written summary
The overexpression line decreased expression of the named OsPIN genes.
- Taxon
- riceOryza sativat0
- Material
- OsTPR1-Oxe0
- Gene
- OsPIN1a, OsPIN1b, OsPIN1c, OsPIN2, and OsPIN5ae0
- Outcome
- expression levelse0
- Direction
- decreasede0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“the expression levels of several auxin efflux transporter genes encoding PIN-FORMED (PIN) proteins, including OsPIN1a, OsPIN1b, OsPIN1c, OsPIN2, and OsPIN5a, were increased in the OsTPR1-Ri line but decreased in the OsTPR1-Ox line”
t0“rice”
Claim · model-written summary
OsTPR1 overexpression significantly increased tiller angle.
- Taxon
- riceOryza sativat0
- Treatment
- OsTPR1 overexpressione0
- Gene
- OsTPR1e0
- Outcome
- tiller anglee0
- Direction
- increasee0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsTPR1 overexpression led to a significant increase in tiller angle and a delay in flowering time”
t0“rice”
Claim · model-written summary
OsTPR1 overexpression delayed flowering.
- Taxon
- riceOryza sativat0
- Treatment
- OsTPR1 overexpressione0
- Gene
- OsTPR1e0
- Outcome
- flowering timee0
- Direction
- delaye0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsTPR1 overexpression led to a significant increase in tiller angle and a delay in flowering time”
t0“rice”
Claim · model-written summary
OsTPR1 RNA interference produced earlier flowering.
- Taxon
- riceOryza sativat0
- Material
- OsTPR1-Rie0
- Outcome
- floweringe0
- Direction
- earliere0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsTPR1-Ri plants exhibited earlier flowering”
t0“rice”
Claim · model-written summary
The authors interpret OsTPR1 as a negative regulator of auxin response.
- Taxon
- riceOryza sativat0
- Gene
- OsTPR1e0
- Outcome
- auxin responsee0
- Qualifier
- negative regulatore0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“OsTPR1 acts as a negative regulator of the auxin response, with its overexpression leading to reduced auxin sensitivity”
t0“rice”
The same questions in other papers
loss of function · growth · Oryza sativa (rice)Positive and negative results
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