Abstract
Salt stress affects plant growth and development to a great extent in establishing biochemical imbalances and inducing oxidative damage. The effort was undertaken to assess the relative effectiveness of chitosan pre- and post-treatments in relieving salt stress in Brassica napus L., a moderately salt-sensitive oilseed crop, with respect to the expression patterns of P5CS, PIP, and APX genes along with proline and protein accumulation. One factorial experiment was performed under controlled conditions, where the plants were exposed to varying NaCl concentrations (50, 100, 150, and 200 mM), following either the pre-treatment of chitosan (50 mg/L) or post-treatment of chitosan (50 and 100 mg/L). Gene expression analysis by means of qRT-PCR showed a significant (p < 0.05) upregulation under pre-treatment of P5CS and APX, thereby enhancing osmoprotection and antioxidant defense; however, under post-treatment, the response of P5CS and APX was only moderate. Post-treatment levels of proline and total protein were markedly higher as well. Interestingly, proline content increased by approximately 743% in pre-treated plants at 200 mM of NaCl compared to the control (p < 0.05), indicating enhanced stress tolerance. Besides, the plants subjected to pre-treatment were perfectly grown at 200 mM NaCl whereas those subjected to post-treatment were unable to survive at 150 mM. These findings reveal that the salt tolerance mechanism is better gained by chitosan pre-treatment due to osmotic adjustment enhancement and antioxidative mechanism. This study presents the potential of chitosan to be developed as a strategic biostimulant in promoting crop resistance to saline conditions.
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Claims 7
Claim · model-written summary
Chitosan pre-treatment significantly upregulated P5CS under salinity.
- Taxon
- Brassica napus L.
_Brassica_napus_-_Habit.jpg?width=60)
Brassica napuse1 - Treatment
- pre-treatmente0
- Gene
- P5CSe0
- Outcome
- upregulatione0
- Direction
- upregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
_Brassica_napus_-_Habit.jpg?width=500)
Verbatim
e0“Gene expression analysis by means of qRT-PCR showed a significant (p < 0.05) upregulation under pre-treatment of P5CS and APX”
e1“Brassica napus L.”
Claim · model-written summary
Chitosan pre-treatment significantly upregulated APX under salinity.
- Taxon
- Brassica napus L.Brassica napuse1
- Treatment
- pre-treatmente0
- Gene
- APXe0
- Outcome
- upregulatione0
- Direction
- upregulatione0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Gene expression analysis by means of qRT-PCR showed a significant (p < 0.05) upregulation under pre-treatment of P5CS and APX”
e1“Brassica napus L.”
Claim · model-written summary
Post-treatment gave only moderate P5CS and APX responses.
- Taxon
- Brassica napus L.Brassica napuse1
- Treatment
- post-treatmente0
- Outcome
- response of P5CS and APXe0
- Qualifier
- only moderatee0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“under post-treatment, the response of P5CS and APX was only moderate”
e1“Brassica napus L.”
Claim · model-written summary
Post-treatment produced markedly higher proline and total protein levels.
- Taxon
- Brassica napus L.Brassica napuse1
- Treatment
- Post-treatmente0
- Outcome
- levels of proline and total proteine0
- Direction
- highere0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Post-treatment levels of proline and total protein were markedly higher as well.”
e1“Brassica napus L.”
Claim · model-written summary
Proline increased approximately 743% in pre-treated plants at 200 mM NaCl relative to control.
- Taxon
- Brassica napus L.Brassica napuse1
- Treatment
- pre-treated plants at 200 mM of NaCle0
- Outcome
- proline contente0
- Direction
- increasede0
- Value
- approximately 743%e0
- Comparator
- controle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“proline content increased by approximately 743% in pre-treated plants at 200 mM of NaCl compared to the control (p < 0.05)”
e1“Brassica napus L.”
Claim · model-written summary
Pre-treated plants grew at 200 mM NaCl.
- Taxon
- Brassica napus L.Brassica napuse1
- Treatment
- pre-treatmente0
- Outcome
- perfectly growne0
- Qualifier
- 200 mM NaCle0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“plants subjected to pre-treatment were perfectly grown at 200 mM NaCl”
e1“Brassica napus L.”
Claim · model-written summary
Post-treated plants failed to survive at 150 mM NaCl.
- Taxon
- Brassica napus L.Brassica napuse1
- Treatment
- post-treatmente0
- Outcome
- unable to survivee0
- Qualifier
- 150 mMe0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“those subjected to post-treatment were unable to survive at 150 mM”
e1“Brassica napus L.”
The same questions in other papers
sodium chloride · physiology: osmolytes · Brassica napus (oilseed rape)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.