Abstract
Abiotic stress greatly inhibits crop growth and reduces yields. However, little is known about the transcriptomic changes that occur in the industrial oilseed crop, rapeseed (Brassica napus), in response to abiotic stress. In this study, we examined the physiological and transcriptional responses of rapeseed to drought (simulated by treatment with 15% (w/v) polyethylene glycol (PEG) 6000) and salinity (150 mM NaCl) stress. Proline contents in young seedlings greatly increased under both conditions after 3 h of treatment, whereas the levels of antioxidant enzymes remained unchanged. We assembled transcripts from the leaves and roots of rapeseed and performed BLASTN searches against the rapeseed genome database for the first time. Gene ontology analysis indicated that DEGs involved in catalytic activity, metabolic process, and response to stimulus were highly enriched. The Kyoto Encyclopedia of Genes and Genomes (KEGG) pathway analysis revealed that differentially expressed genes (DEGs) from the categories metabolic pathways and biosynthesis of secondary metabolites were highly enriched. We determined that myeloblastosis (MYB), NAM/ATAF1-2/CUC2 (NAC), and APETALA2/ethylene-responsive element binding proteins (AP2-EREBP) transcription factors function as major switches that control downstream gene expression and that proline plays a role under short-term abiotic stress treatment due to increased expression of synthesis and decreased expression of degradation. Furthermore, many common genes function in the response to both types of stress in this rapeseed.
Abstract from the paper’s OpenAlex record; open copy licensed cc-by. Read at the source ↗
The text the model read, with the source record’s inline markup (italics, sub- and superscripts) rendered and its character entities decoded; quote offsets refer to the raw text.
Claims 8
Claim · model-written summary
Drought treatment increased seedling proline after three hours.
- Taxon
- rapeseede2
- Treatment
- droughte1
- Outcome
- Proline contentse0
- Direction
- increasede0
- Qualifier
- after 3 h of treatmente0
Not reportedComparator
Verbatim
e0“Proline contents in young seedlings greatly increased under both conditions after 3 h of treatment”
e1“drought (simulated by treatment with 15% (w/v) polyethylene glycol (PEG) 6000)”
e2“rapeseed (Brassica napus)”
Claim · model-written summary
Salinity treatment increased seedling proline after three hours.
Not reportedComparator
Verbatim
e0“Proline contents in young seedlings greatly increased under both conditions after 3 h of treatment”
e1“salinity (150 mM NaCl) stress”
e2“rapeseed (Brassica napus)”
Claim · model-written summary
Antioxidant enzyme levels remained unchanged under both stresses.
Verbatim
e0“the levels of antioxidant enzymes remained unchanged”
e1“rapeseed (Brassica napus)”
Claim · model-written summary
Gene ontology analysis found enrichment of DEGs for catalytic activity, metabolism and stimulus response.
Verbatim
e0“DEGs involved in catalytic activity, metabolic process, and response to stimulus were highly enriched”
e1“rapeseed (Brassica napus)”
Claim · model-written summary
KEGG analysis found enrichment of DEGs for metabolic pathways and secondary metabolite biosynthesis.
Verbatim
e0“differentially expressed genes (DEGs) from the categories metabolic pathways and biosynthesis of secondary metabolites were highly enriched”
e1“rapeseed (Brassica napus)”
Claim · model-written summary
MYB, NAC and AP2-EREBP factors were inferred to control downstream gene expression.
Verbatim
e0“myeloblastosis (MYB), NAM/ATAF1-2/CUC2 (NAC), and APETALA2/ethylene-responsive element binding proteins (AP2-EREBP) transcription factors function as major switches that control downstream gene expression”
e1“rapeseed (Brassica napus)”
Claim · model-written summary
Proline accumulation under short-term stress was linked to greater synthesis and lower degradation expression.
Verbatim
e0“proline plays a role under short-term abiotic stress treatment due to increased expression of synthesis and decreased expression of degradation”
e1“rapeseed (Brassica napus)”
Claim · model-written summary
Many genes were common to responses to both stresses.
Verbatim
e0“many common genes function in the response to both types of stress in this rapeseed”
The same questions in other papers
sodium chloride · physiology: osmolytes · Brassica napus (oilseed rape)Results agree
5 papers in the same direction as this paper
- positive
- positive
- positive
2 papers more in this group
See all 6 papers →salinity · physiology: osmolytes · Brassica napus (oilseed rape)Results differ
2 papers in the same direction as this paper · 6 in the opposite direction
- negative
- negative
- negative
5 papers more in this group
See all 9 papers →drought · physiology: osmolytes · Brassica napus (oilseed rape)Results differ
7 papers in the same direction as this paper · 7 in the opposite direction
- negative
- negative
- negative
11 papers more in this group
See all 15 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.