Paper · 2025 · Ciência Rural

Tetrazolium test and embryonic development stages in cataia seeds

Bianca Lamounier da Silva Lima; Luciana Magda de Oliveira; Isabelle da Silva Wolff

9
claims
19
verbatim quotes
0
citations
5
research areas
✓
Open access · cc-by-nc

Abstract

ABSTRACT: This study proposed a tetrazolium (TZ) test methodology for cataia (Drimys brasiliensis Miers) seeds and assess the efficiency of stratification in promoting development in dormant embryos. Fruits were collected in three municipalities in Santa Catarina State (SC), Brazil, the seeds extracted, and their moisture content determined. Different soaking times in the tetrazolium solution were tested and the seeds submitted to treatment with and without stratification, followed by germination testing. The TZ was performed on ungerminated seeds and the embryos were classified according to development stages. Seed moisture content ranged from 10.6 to 11.9%. The results indicated that soaking in 0.7% tetrazolium for 48 and 72 h was sufficient to assess seed viability, with the latter time facilitating assessment. Seed germination was 0 and 6% without and with stratification, respectively. Although, 94% of the unstratified seeds were viable, most of the embryos were immature; whereas, 89% of the stratified seeds were viable but only 3% mature. It was concluded that longitudinal sectioning and soaking in a 0.7% tetrazolium solution, at 25 °C in the dark for 72 h is an appropriate method for assessing the viability of cataia seeds. Stratification for 60 days did not overcome morphological dormancy.

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Claims 9

Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

Soaking in 0.7% tetrazolium for 48 and 72 h was sufficient to assess cataia seed viability.

Taxon
Drimys brasiliensisDrimys brasiliensise1
Treatment
soaking in 0.7% tetrazoliume2
Outcome
seed viabilitye2
Value
48 and 72 he2
Qualifier
sufficiente2

Verbatim

e1

“This study proposed a tetrazolium (TZ) test methodology for cataia (Drimys brasiliensis Miers) seeds”

✓ located verbatim in the source · abstract, characters 10–110 · show in text ↑
e2

“The results indicated that soaking in 0.7% tetrazolium for 48 and 72 h was sufficient to assess seed viability, with the latter time facilitating assessment”

✓ located verbatim in the source · abstract, characters 668–824 · show in text ↑
Method / resourceobservedoriginal resultpositivemeasured

Claim · model-written summary

A 72 h tetrazolium soak facilitated the assessment of cataia seed viability compared with 48 h.

Taxon
Drimys brasiliensisDrimys brasiliensise1
Outcome
facilitating assessmente2
Value
72 he2
Qualifier
with the latter timee2

Verbatim

e1

“This study proposed a tetrazolium (TZ) test methodology for cataia (Drimys brasiliensis Miers) seeds”

✓ located verbatim in the source · abstract, characters 10–110 · show in text ↑
e2

“The results indicated that soaking in 0.7% tetrazolium for 48 and 72 h was sufficient to assess seed viability, with the latter time facilitating assessment”

✓ located verbatim in the source · abstract, characters 668–824 · show in text ↑
Recommendationauthor interpretationoriginal resultpositivebasis n/a

Claim · model-written summary

Longitudinal sectioning and soaking in 0.7% tetrazolium at 25 °C in the dark for 72 h is recommended for assessing cataia seed viability.

Taxon
Drimys brasiliensisDrimys brasiliensise1
Treatment
longitudinal sectioning and soaking in a 0.7% tetrazolium solution, at 25 °C in the dark for 72 he3
Outcome
method for assessing the viability of cataia seedse3

Verbatim

e1

“This study proposed a tetrazolium (TZ) test methodology for cataia (Drimys brasiliensis Miers) seeds”

✓ located verbatim in the source · abstract, characters 10–110 · show in text ↑
e3

“It was concluded that longitudinal sectioning and soaking in a 0.7% tetrazolium solution, at 25 °C in the dark for 72 h is an appropriate method for assessing the viability of cataia seeds”

✓ located verbatim in the source · abstract, characters 1060–1248 · show in text ↑
Intervention effectobservedoriginal resultpositivemeasured

Claim · model-written summary

Stratification increased cataia seed germination from 0% to 6%.

Taxon
Drimys brasiliensisDrimys brasiliensise1
Treatment
with stratificatione4
Outcome
Seed germinatione4
Direction
increaseinferrede4
Value
0 and 6%e4
Comparator
unstratifiede5

Verbatim

e1

“This study proposed a tetrazolium (TZ) test methodology for cataia (Drimys brasiliensis Miers) seeds”

✓ located verbatim in the source · abstract, characters 10–110 · show in text ↑
e4

“Seed germination was 0 and 6% without and with stratification, respectively”

✓ located verbatim in the source · abstract, characters 826–901 · show in text ↑
e5

“Although, 94% of the unstratified seeds were viable, most of the embryos were immature”

✓ located verbatim in the source · abstract, characters 903–989 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Of unstratified cataia seeds, 94% were viable.

Taxon
Drimys brasiliensisDrimys brasiliensise1
Outcome
viablee5
Value
94%e5
Qualifier
unstratified seedse5

Verbatim

e1

“This study proposed a tetrazolium (TZ) test methodology for cataia (Drimys brasiliensis Miers) seeds”

✓ located verbatim in the source · abstract, characters 10–110 · show in text ↑
e5

“Although, 94% of the unstratified seeds were viable, most of the embryos were immature”

✓ located verbatim in the source · abstract, characters 903–989 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Most embryos of unstratified cataia seeds were immature.

Taxon
Drimys brasiliensisDrimys brasiliensise1
Organ / stage
embryose5
Outcome
immaturee5
Qualifier
unstratified seedse5

Verbatim

e1

“This study proposed a tetrazolium (TZ) test methodology for cataia (Drimys brasiliensis Miers) seeds”

✓ located verbatim in the source · abstract, characters 10–110 · show in text ↑
e5

“Although, 94% of the unstratified seeds were viable, most of the embryos were immature”

✓ located verbatim in the source · abstract, characters 903–989 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Of stratified cataia seeds, 89% were viable.

Taxon
Drimys brasiliensisDrimys brasiliensise1
Outcome
viablee6
Value
89%e6
Qualifier
stratified seedse6

Verbatim

e1

“This study proposed a tetrazolium (TZ) test methodology for cataia (Drimys brasiliensis Miers) seeds”

✓ located verbatim in the source · abstract, characters 10–110 · show in text ↑
e6

“89% of the stratified seeds were viable but only 3% mature”

✓ located verbatim in the source · abstract, characters 1000–1058 · show in text ↑
Observationobservedoriginal resultmeasured

Claim · model-written summary

Only 3% of stratified cataia seeds had mature embryos.

Taxon
Drimys brasiliensisDrimys brasiliensise1
Outcome
maturee6
Value
3%e6
Qualifier
stratified seedse6

Verbatim

e1

“This study proposed a tetrazolium (TZ) test methodology for cataia (Drimys brasiliensis Miers) seeds”

✓ located verbatim in the source · abstract, characters 10–110 · show in text ↑
e6

“89% of the stratified seeds were viable but only 3% mature”

✓ located verbatim in the source · abstract, characters 1000–1058 · show in text ↑
Intervention effectobservedoriginal resultnegativemeasured

Claim · model-written summary

Stratification for 60 days did not overcome morphological dormancy in cataia seeds.

Taxon
Drimys brasiliensisDrimys brasiliensise1
Treatment
Stratification for 60 dayse7
Outcome
morphological dormancye7
Direction
did not overcomee7

Not reportedComparator

Verbatim

e1

“This study proposed a tetrazolium (TZ) test methodology for cataia (Drimys brasiliensis Miers) seeds”

✓ located verbatim in the source · abstract, characters 10–110 · show in text ↑
e7

“Stratification for 60 days did not overcome morphological dormancy”

✓ located verbatim in the source · abstract, characters 1250–1316 · show in text ↑