Abstract
• ScCRT and ScCNX family members from the sugarcane cultivar XTT22 were identified. • CNXs interacts with potyviral 6K2, whereas CRTs do not. • Transient expression of ScCNX1-1 or silencing of NbCNXs inhibited TuMV infection. • ScCNX1-1 is a potential target for engineering SCMV resistance in sugarcane. Sugarcane mosaic disease, primarily caused by sugarcane mosaic virus (SCMV), seriously affects sugarcane production. The 6K2 protein is crucial for potyvirus infection. Calnexin (CNX) and calreticulin (CRT) play key roles in plants growth, development, and response to stresses. However, the CRT and CNX families have not been studied in sugarcane. Here, we screened CNX from a sugarcane cDNA yeast library with 6K2 of SCMV as bait. We identified 65 ScCRTs and 11 ScCNXs from the sugarcane cultivar XTT22, which were phylogenetically classified into three groups. The promoter regions of these genes contain multiple cis -elements related to growth and development, environmental stress, plant hormone responses, and light responses. Transcriptomic analysis revealed differential spatiotemporal expression of ScCRTs and ScCNXs . We cloned four CRT and one CNX genes from XTT22, which localized to the endoplasmic reticulum. ScCNX1-1 was differentially expressed in sugarcane tissues and was upregulated upon SCMV infection. Protein–protein assays demonstrated that only ScCNX1-1 interacts with SCMV-6K2. Transient expression of ScCNX1-1 in Nicotiana benthamiana suppressed turnip mosaic virus (TuMV) infection. Moreover, all four CNXs, but not the CRTs from N. benthamiana , interacted with TuMV-6K2. Transient NbCNX silencing inhibited TuMV infection. These findings indicate that the interaction of CNX with 6K2 is a conserved mechanism for potyvirus infection, and that CNX expression is fine-tuned and tightly controlled upon viral infection. This study sheds light on the roles of ScCRT and ScCNX genes in sugarcane growth, development, and response to stresses, and provides a potential molecular target for engineering SCMV resistance in sugarcane.
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Claims 13
Claim · model-written summary
The sugarcane cultivar XTT22 had 65 ScCRTs.
Verbatim
e0“We identified 65 ScCRTs and 11 ScCNXs from the sugarcane cultivar XTT22”
Claim · model-written summary
The sugarcane cultivar XTT22 had 11 ScCNXs.
Verbatim
e0“We identified 65 ScCRTs and 11 ScCNXs from the sugarcane cultivar XTT22”
Claim · model-written summary
The identified genes were classified into three phylogenetic groups.
Verbatim
e0“which were phylogenetically classified into three groups”
t0“sugarcane”
Claim · model-written summary
The promoters contained cis-elements for growth, stress, hormone and light responses.
Verbatim
e0“The promoter regions of these genes contain multiple cis -elements related to growth and development, environmental stress, plant hormone responses, and light responses”
t0“sugarcane”
Claim · model-written summary
ScCRTs and ScCNXs showed differential spatiotemporal expression.
Verbatim
e0“Transcriptomic analysis revealed differential spatiotemporal expression of ScCRTs and ScCNXs”
t0“sugarcane”
Claim · model-written summary
Cloned CRT and CNX genes from XTT22 localized to the endoplasmic reticulum.
Verbatim
e0“We cloned four CRT and one CNX genes from XTT22, which localized to the endoplasmic reticulum”
t0“sugarcane”
Claim · model-written summary
ScCNX1-1 was differentially expressed across sugarcane tissues.
Verbatim
e0“ScCNX1-1 was differentially expressed in sugarcane tissues”
Claim · model-written summary
SCMV infection upregulated ScCNX1-1.
- Taxon
- sugarcanee0
- Treatment
- SCMV infectione0
- Interactor
- SCMV
Sugarcane mosaic viruse0pathogen - Gene
- ScCNX1-1e0
- Outcome
- ScCNX1-1e0
- Direction
- upregulatede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“ScCNX1-1 was differentially expressed in sugarcane tissues and was upregulated upon SCMV infection”
Claim · model-written summary
Only ScCNX1-1 interacted with SCMV-6K2 in protein assays.
- Taxon
- sugarcanet0
- Interactor
- SCMVSugarcane mosaic viruse0pathogen
- Gene
- ScCNX1-1e0
- Outcome
- interacts with SCMV-6K2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“only ScCNX1-1 interacts with SCMV-6K2”
t0“sugarcane”
Claim · model-written summary
Transient ScCNX1-1 expression suppressed TuMV infection in Nicotiana benthamiana.
- Taxon
- Nicotiana benthamiana
Nicotiana benthamianae0 - Treatment
- Transient expression of ScCNX1-1e0
- Interactor
- turnip mosaic virus (TuMV)
Turnip mosaic viruse0pathogen - Gene
- ScCNX1-1e0
- Outcome
- turnip mosaic virus (TuMV) infectione0
- Direction
- suppressede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient expression of ScCNX1-1 in Nicotiana benthamiana suppressed turnip mosaic virus (TuMV) infection”
Claim · model-written summary
All four CNXs from N. benthamiana interacted with TuMV-6K2.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Interactor
- TuMVTurnip mosaic viruse0pathogen
- Gene
- CNXse0
- Outcome
- interacted with TuMV-6K2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all four CNXs, but not the CRTs from N. benthamiana , interacted with TuMV-6K2”
Claim · model-written summary
CRTs from N. benthamiana did not interact with TuMV-6K2.
- Taxon
- N. benthamianaNicotiana benthamianae0
- Interactor
- TuMVTurnip mosaic viruse0pathogen
- Gene
- CRTse0
- Outcome
- not the CRTs from N. benthamiana , interacted with TuMV-6K2e0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“all four CNXs, but not the CRTs from N. benthamiana , interacted with TuMV-6K2”
Claim · model-written summary
Transient NbCNX silencing inhibited TuMV infection.
- Taxon
- N. benthamianaNicotiana benthamianae1
- Treatment
- Transient NbCNX silencinge0
- Interactor
- TuMVTurnip mosaic viruse0pathogen
- Gene
- NbCNXe0
- Outcome
- TuMV infectione0
- Direction
- inhibitede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“Transient NbCNX silencing inhibited TuMV infection”
e1“the CRTs from N. benthamiana”
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Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.