Abstract
Sulfur dioxide-based preservation is the most widely used and effective method for storing table grapes ( Vitis vinifera L.), triggering a transcriptional response of the defense-related genes. In this study, we selected a previously uncharacterized SO 2 -responsive gene, VvWRKY72 , from RNA-seq data to characterize it using transgenic Arabidopsis and grape plants. The VvWRKY72 gene encodes a 605-amino acid protein with one WRKY domain belonging to the group IIb WRKY subfamily. VvWRKY72 was expressed in various grape tissues; however, higher levels of expression were detected in roots, mature leaves, and mature fruit skins. The VvWRKY72 gene responded to low temperature, phytohormone methyl jasmonate, and Botrytis cinerea . In transgenic Arabidopsis plants overexpressing VvWRKY72 , VvWRKY72-GFP was localized in the nucleus . B. cinerea caused severe yellowing and necrotic lesions in leaves of transgenic Arabidopsis plants overexpressing VvWRKY72 , which were accompanied by reduced antioxidant enzyme activities, elevated levels of H₂O₂ and malondialdehyde (MDA), and increased cell death. Meanwhile, the transgenic plants exhibited decreased callose deposition, heightened activities of plant cell wall-degrading enzymes (PCWDEs), and downregulated jasmonic acid (JA) signaling genes ( AtLOX3, AtAOC3, AtOPR3, AtORA59, AtPDF1.2 , and AtPR3 ), as well as related chitinase (CHI) activity following B. cinerea inoculation. Similarly, after inoculation with B. cinerea , the leaves of VvWRKY72 -overexpressing grape plants exhibited severe disease symptoms, along with downregulation of antioxidant and JA-responsive genes, and upregulation of genes encoding PCWDEs. Conversely, silencing of VvWRKY72 enhanced resistance of grape plant to B. cinerea . Therefore, we speculated that VvWRKY72 played negative regulatory roles in B. cinerea infection by impairing reactive oxygen species (ROS) homeostasis, JA-mediated defense and cell wall-associated defenses.
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Claims 20
Claim · model-written summary
VvWRKY72 was expressed in grape tissues, with higher levels in roots, mature leaves and mature fruit skins.
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“VvWRKY72 was expressed in various grape tissues; however, higher levels of expression were detected in roots, mature leaves, and mature fruit skins”
Claim · model-written summary
The VvWRKY72 gene encodes a 605-amino acid protein with one WRKY domain of group IIb.
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“The VvWRKY72 gene encodes a 605-amino acid protein with one WRKY domain belonging to the group IIb WRKY subfamily”
Claim · model-written summary
VvWRKY72-GFP was localized in the nucleus of transgenic Arabidopsis.
- Taxon
- Arabidopsis
Arabidopsist0 - Gene
- VvWRKY72-GFPe0
- Outcome
- localized in the nucleuse0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“VvWRKY72-GFP was localized in the nucleus”
Claim · model-written summary
Botrytis cinerea caused severe yellowing and necrotic lesions in leaves of transgenic Arabidopsis overexpressing VvWRKY72.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing VvWRKY72e0
- Interactor
- B. cinerea
Botrytis cinereae0pathogen - Outcome
- yellowing and necrotic lesionse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“B. cinerea caused severe yellowing and necrotic lesions in leaves of transgenic Arabidopsis plants overexpressing VvWRKY72”
Claim · model-written summary
Overexpressing VvWRKY72 in Arabidopsis reduced antioxidant enzyme activities after B. cinerea infection.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing VvWRKY72e0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- antioxidant enzyme activitiese1
- Direction
- reducede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“B. cinerea caused severe yellowing and necrotic lesions in leaves of transgenic Arabidopsis plants overexpressing VvWRKY72”
e1“reduced antioxidant enzyme activities, elevated levels of H₂O₂ and malondialdehyde (MDA), and increased cell death”
Claim · model-written summary
Overexpressing VvWRKY72 in Arabidopsis elevated H₂O₂ and malondialdehyde after B. cinerea infection.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing VvWRKY72e0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- H₂O₂ and malondialdehyde (MDA)e1
- Direction
- elevatede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“B. cinerea caused severe yellowing and necrotic lesions in leaves of transgenic Arabidopsis plants overexpressing VvWRKY72”
e1“reduced antioxidant enzyme activities, elevated levels of H₂O₂ and malondialdehyde (MDA), and increased cell death”
Claim · model-written summary
Overexpressing VvWRKY72 in Arabidopsis increased cell death after B. cinerea infection.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing VvWRKY72e0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- cell deathe1
- Direction
- increasede1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“B. cinerea caused severe yellowing and necrotic lesions in leaves of transgenic Arabidopsis plants overexpressing VvWRKY72”
e1“reduced antioxidant enzyme activities, elevated levels of H₂O₂ and malondialdehyde (MDA), and increased cell death”
Claim · model-written summary
Overexpressing VvWRKY72 in Arabidopsis decreased callose deposition.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing VvWRKY72e0
- Outcome
- callose depositione2
- Direction
- decreasede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“B. cinerea caused severe yellowing and necrotic lesions in leaves of transgenic Arabidopsis plants overexpressing VvWRKY72”
e2“the transgenic plants exhibited decreased callose deposition, heightened activities of plant cell wall-degrading enzymes (PCWDEs)”
Claim · model-written summary
Overexpressing VvWRKY72 in Arabidopsis heightened activities of plant cell wall-degrading enzymes.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing VvWRKY72e0
- Outcome
- activities of plant cell wall-degrading enzymes (PCWDEs)e2
- Direction
- heightenede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“B. cinerea caused severe yellowing and necrotic lesions in leaves of transgenic Arabidopsis plants overexpressing VvWRKY72”
e2“the transgenic plants exhibited decreased callose deposition, heightened activities of plant cell wall-degrading enzymes (PCWDEs)”
Claim · model-written summary
Overexpressing VvWRKY72 in Arabidopsis downregulated the JA signalling genes AtLOX3, AtAOC3 and AtOPR3.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing VvWRKY72e0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- AtLOX3, AtAOC3, AtOPR3e3
- Outcome
- jasmonic acid (JA) signaling genese3
- Direction
- downregulatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“B. cinerea caused severe yellowing and necrotic lesions in leaves of transgenic Arabidopsis plants overexpressing VvWRKY72”
e3“downregulated jasmonic acid (JA) signaling genes ( AtLOX3, AtAOC3, AtOPR3, AtORA59, AtPDF1.2 , and AtPR3 )”
Claim · model-written summary
Overexpressing VvWRKY72 in Arabidopsis downregulated the JA signalling genes AtORA59, AtPDF1.2 and AtPR3.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing VvWRKY72e0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- AtORA59, AtPDF1.2 , and AtPR3e3
- Outcome
- jasmonic acid (JA) signaling genese3
- Direction
- downregulatede3
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“B. cinerea caused severe yellowing and necrotic lesions in leaves of transgenic Arabidopsis plants overexpressing VvWRKY72”
e3“downregulated jasmonic acid (JA) signaling genes ( AtLOX3, AtAOC3, AtOPR3, AtORA59, AtPDF1.2 , and AtPR3 )”
Claim · model-written summary
Overexpressing VvWRKY72 in Arabidopsis had related chitinase activity after B. cinerea inoculation.
- Taxon
- ArabidopsisArabidopsist0
- Treatment
- overexpressing VvWRKY72e0
- Interactor
- B. cinereaBotrytis cinereae4pathogen
- Outcome
- chitinase (CHI) activitye4
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“B. cinerea caused severe yellowing and necrotic lesions in leaves of transgenic Arabidopsis plants overexpressing VvWRKY72”
e4“as well as related chitinase (CHI) activity following B. cinerea inoculation”
Claim · model-written summary
After B. cinerea inoculation, VvWRKY72-overexpressing grape plants showed severe disease symptoms.
- Taxon
- grape plantse0
- Treatment
- VvWRKY72 -overexpressinge0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- severe disease symptomse0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after inoculation with B. cinerea , the leaves of VvWRKY72 -overexpressing grape plants exhibited severe disease symptoms”
Claim · model-written summary
VvWRKY72-overexpressing grape plants downregulated antioxidant and JA-responsive genes after B. cinerea inoculation.
- Taxon
- grape plantse0
- Treatment
- VvWRKY72 -overexpressinge0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- antioxidant and JA-responsive genese1
- Direction
- downregulatione1
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after inoculation with B. cinerea , the leaves of VvWRKY72 -overexpressing grape plants exhibited severe disease symptoms”
e1“along with downregulation of antioxidant and JA-responsive genes”
Claim · model-written summary
VvWRKY72-overexpressing grape plants upregulated genes encoding PCWDEs after B. cinerea inoculation.
- Taxon
- grape plantse0
- Treatment
- VvWRKY72 -overexpressinge0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- genes encoding PCWDEse2
- Direction
- upregulatione2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“after inoculation with B. cinerea , the leaves of VvWRKY72 -overexpressing grape plants exhibited severe disease symptoms”
e2“upregulation of genes encoding PCWDEs”
Claim · model-written summary
Silencing VvWRKY72 enhanced resistance of grape plants to Botrytis cinerea.
- Taxon
- grape plante0
- Treatment
- silencing of VvWRKY72e0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Outcome
- resistancee0
- Direction
- enhancede0
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e0“silencing of VvWRKY72 enhanced resistance of grape plant to B. cinerea”
Claim · model-written summary
VvWRKY72 may play negative regulatory roles in B. cinerea infection of grape by impairing ROS homeostasis, JA-mediated defence and cell wall-associated defences.
- Taxon
- grape plantst0
- Interactor
- B. cinereaBotrytis cinereae0pathogen
- Gene
- VvWRKY72e0
- Outcome
- negative regulatory roles in B. cinerea infectione0
- Qualifier
- impairing reactive oxygen species (ROS) homeostasis, JA-mediated defense and cell wall-associated defensese0
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“we speculated that VvWRKY72 played negative regulatory roles in B. cinerea infection by impairing reactive oxygen species (ROS) homeostasis, JA-mediated defense and cell wall-associated defenses”
Claim · model-written summary
The VvWRKY72 gene responded to low temperature in grape.
Not reportedComparatorDirection
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“The VvWRKY72 gene responded to low temperature, phytohormone methyl jasmonate, and Botrytis cinerea”
Claim · model-written summary
The VvWRKY72 gene responded to phytohormone methyl jasmonate in grape.
Not reportedComparatorDirection
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“The VvWRKY72 gene responded to low temperature, phytohormone methyl jasmonate, and Botrytis cinerea”
Claim · model-written summary
The VvWRKY72 gene responded to Botrytis cinerea in grape.
- Taxon
- grape plantst0
- Treatment
- Botrytis cinereae0
- Interactor
- Botrytis cinereaBotrytis cinereae0pathogen
- Gene
- VvWRKY72e0
- Outcome
- respondede0
Not reportedComparatorDirection
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
t0“transgenic Arabidopsis and grape plants”
e0“The VvWRKY72 gene responded to low temperature, phytohormone methyl jasmonate, and Botrytis cinerea”
The same questions in other papers
loss of function · Botrytis cinerea · VitisPositive and negative results
2 papers in the same direction as this paper · 2 in the opposite direction
- negative
- negative
- positive
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See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.