Abstract
Fusarium oxysporum is the causal agent of Fusarium dry rot disease (FDR), which results in postharvest potato losses. Biological control agents (BCAs) and phytohormone melatonin have antifungal effects on fungal pathogens and can be used as alternatives to synthetic chemical fungicides. The aim of the study was to evaluate the integrated effects of melatonin (MEL) and Bacillus amyloliquefaciens (Bamy) on the postharvest quality of potato tubers. Bamy was integrated with six MEL concentrations and screened against F. oxysporum in vitro and in vivo. The effects of the best performing treatment were evaluated for antioxidant activity, phenolic content, ascorbic acid content, and protein content on potato tubers. In the in vitro screening trial, treatment with Bamy + MEL100 had the highest mycelial growth inhibition percentage (59.92%), followed by Bamy + MEL15 and Bamy + MEL50 (56.12% and 55.27%, respectively). Potato tubers treated with Bamy + MEL100 had the lowest disease severity of FDR (50.61%) and a pathogen penetration value of 6.39 mm. Bamy + MEL50 showed a disease severity percentage of 59.72%. The exogenous application of melatonin at a concentration of 100 µM combined with B. amyloliquefaciens was the most effective treatment with the highest phenolic content (144.1 mg GAE/g DW) and protein content (68 mg/g DM) compared to the untreated tubers (104.4 GAE/g DW phenolic content and 50.06 mg/g DM protein content). Tubers treated with melatonin had the highest ascorbic acid content (5.48 mg AAE/100g DM) compared to the untreated tubers (4.09 mg AAE/100 g DM). Overall, tubers treated with a combination of melatonin and B. amyloliquefaciens showed less severe symptoms of FDR across all concentrations. Based on these findings, it can be concluded that B. amyloliquefaciens and melatonin at 100 µM concentration can be used in combination to inhibit the growth of F. oxysporum.
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Claims 11
Claim · model-written summary
Bamy plus MEL100 inhibited fungal mycelial growth by 59.92%.
- Taxon
- Potato Tuberst0
- Treatment
- Bamy + MEL100e0
- Outcome
- mycelial growth inhibition percentagee0
- Direction
- higheste0
- Value
- 59.92%e0
Not reportedComparator
Verbatim
e0“treatment with Bamy + MEL100 had the highest mycelial growth inhibition percentage (59.92%)”
t0“Potato Tubers”
Claim · model-written summary
Bamy plus MEL15 inhibited mycelial growth by 56.12%.
- Taxon
- Potato Tuberst0
- Treatment
- Bamy + MEL15e0
- Outcome
- mycelial growth inhibition percentagee0
- Direction
- lowerinferrede0
- Value
- 56.12%e0
Not reportedComparator
Verbatim
e0“treatment with Bamy + MEL100 had the highest mycelial growth inhibition percentage (59.92%), followed by Bamy + MEL15 and Bamy + MEL50 (56.12% and 55.27%, respectively)”
t0“Potato Tubers”
Claim · model-written summary
Bamy plus MEL50 inhibited mycelial growth by 55.27%.
- Taxon
- Potato Tuberst0
- Treatment
- Bamy + MEL50e0
- Outcome
- mycelial growth inhibition percentagee0
- Direction
- lowerinferrede0
- Value
- 55.27%e0
Not reportedComparator
Verbatim
e0“treatment with Bamy + MEL100 had the highest mycelial growth inhibition percentage (59.92%), followed by Bamy + MEL15 and Bamy + MEL50 (56.12% and 55.27%, respectively)”
t0“Potato Tubers”
Claim · model-written summary
Bamy plus MEL100 gave the lowest dry rot severity at 50.61%.
- Taxon
- Potato tuberse0
- Treatment
- Bamy + MEL100e0
- Outcome
- disease severity of FDRe0
- Direction
- loweste0
- Value
- 50.61%e0
Not reportedComparator
Verbatim
e0“Potato tubers treated with Bamy + MEL100 had the lowest disease severity of FDR (50.61%)”
Claim · model-written summary
Bamy plus MEL100 gave pathogen penetration of 6.39 mm.
Verbatim
e0“a pathogen penetration value of 6.39 mm”
t0“Potato Tubers”
Claim · model-written summary
Bamy plus MEL50 gave dry rot severity of 59.72%.
Verbatim
e0“Bamy + MEL50 showed a disease severity percentage of 59.72%”
t0“Potato Tubers”
Claim · model-written summary
Melatonin and Bacillus at 100 µM gave the highest phenolic content of 144.1 mg GAE/g DW.
- Taxon
- Potato Tuberst0
- Treatment
- melatonin at a concentration of 100 µM combined with B. amyloliquefaciense0
- Outcome
- phenolic contente0
- Direction
- higheste0
- Value
- 144.1 mg GAE/g DWe0
Not reportedComparator
Verbatim
e0“melatonin at a concentration of 100 µM combined with B. amyloliquefaciens was the most effective treatment with the highest phenolic content (144.1 mg GAE/g DW)”
t0“Potato Tubers”
Claim · model-written summary
Melatonin and Bacillus at 100 µM gave protein content of 68 mg/g DM.
Verbatim
e0“melatonin at a concentration of 100 µM combined with B. amyloliquefaciens was the most effective treatment with the highest phenolic content (144.1 mg GAE/g DW) and protein content (68 mg/g DM) compared to the untreated tubers”
t0“Potato Tubers”
Claim · model-written summary
Melatonin-treated tubers had the highest ascorbic acid content of 5.48 mg AAE/100g DM.
Verbatim
e0“Tubers treated with melatonin had the highest ascorbic acid content (5.48 mg AAE/100g DM) compared to the untreated tubers”
t0“Potato Tubers”
Claim · model-written summary
Combined treatment reduced dry rot symptoms at all concentrations.
- Taxon
- Potato Tuberst0
- Treatment
- combination of melatonin and B. amyloliquefaciense0
- Outcome
- symptoms of FDRe0
- Direction
- less severee0
- Qualifier
- across all concentrationse0
Not reportedComparator
Verbatim
e0“tubers treated with a combination of melatonin and B. amyloliquefaciens showed less severe symptoms of FDR across all concentrations”
t0“Potato Tubers”
Claim · model-written summary
The authors conclude that combined Bacillus and melatonin at 100 µM can inhibit Fusarium growth.
Verbatim
e0“B. amyloliquefaciens and melatonin at 100 µM concentration can be used in combination to inhibit the growth of F. oxysporum”
t0“Potato Tubers”
The same questions in other papers
Bacillus amyloliquefaciens · disease · Solanum tuberosum (potato)Positive and negative results
1 paper in the same direction as this paper · 1 in the opposite direction
- no effect
- positive
melatonin · quality: phytochemicals · Solanum tuberosum (potato)Results agree
1 paper in the same direction as this paper
- positive
melatonin · disease · Solanum tuberosum (potato)Results agree
4 papers in the same direction as this paper
- positive
- positive
- positive
1 paper more in this group
See all 5 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.