Abstract
Summary Chilling stress induces photosystem I (PSI) photoinhibition in various plants, severely impairing their growth. However, the mechanisms suppressing chilling‐induced PSI photoinhibition remain unclear. To identify factors preventing PSI photoinhibition, we compared two cucumber cultivars with different susceptibilities to PSI photoinhibition and chilling stress tolerance. Chilling stress caused uncoupling of thylakoid membrane in both cultivars, as previously reported. However, in the chilling‐sensitive cultivar, electron efflux from ferredoxin (Fd) was more restricted under chilling stress, resulting in over‐reduction of PSI. This over‐reduction was observed not only under chilling stress but also under limited CO 2 condition, suggesting that the lower alternative electron flow activity contributes to the cultivar difference in PSI photoinhibition. Indeed, the chilling‐sensitive cultivar lost the activity of the chloroplast NADH dehydrogenase‐like complex (NDH) due to destabilization of the PSI–NDH supercomplex under chilling stress, resulting in severe Fd over‐reduction. By contrast, the chilling‐tolerant cultivar maintained NDH activity and suppressed reactive oxygen species and PSI photoinhibition during chilling stress. This study provides evidence that NDH functions as a crucial electron sink to prevent PSI photoinhibition and provides new insights into the mechanisms underlying low‐temperature stress tolerance.
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Claims 8
Claim · model-written summary
Under chilling stress, electron efflux from ferredoxin was more restricted in the chilling-sensitive cultivar.
- Taxon
- cucumber
Cucumis sativuse7 - Material
- chilling‐sensitive cultivare2
- Treatment
- chilling stresse2
- Outcome
- electron efflux from ferredoxin (Fd)e2
- Direction
- more restrictede2
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“we compared two cucumber cultivars with different susceptibilities to PSI photoinhibition and chilling stress tolerance”
e2“in the chilling‐sensitive cultivar, electron efflux from ferredoxin (Fd) was more restricted under chilling stress, resulting in over‐reduction of PSI”
Claim · model-written summary
Over-reduction was observed under limited CO2 as well as under chilling stress.
- Taxon
- cucumberCucumis sativuse7
- Outcome
- over‐reductione3
- Qualifier
- under limited CO 2 conditione3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“we compared two cucumber cultivars with different susceptibilities to PSI photoinhibition and chilling stress tolerance”
e3“This over‐reduction was observed not only under chilling stress but also under limited CO 2 condition, suggesting that the lower alternative electron flow activity contributes to the cultivar difference in PSI photoinhibition”
Claim · model-written summary
Lower alternative electron flow activity may contribute to the cultivar difference in PSI photoinhibition.
- Taxon
- cucumberCucumis sativuse7
- Outcome
- PSI photoinhibitione3
- Qualifier
- the lower alternative electron flow activitye3
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“we compared two cucumber cultivars with different susceptibilities to PSI photoinhibition and chilling stress tolerance”
e3“This over‐reduction was observed not only under chilling stress but also under limited CO 2 condition, suggesting that the lower alternative electron flow activity contributes to the cultivar difference in PSI photoinhibition”
Claim · model-written summary
The chilling-sensitive cultivar lost NDH activity under chilling stress.
- Taxon
- cucumberCucumis sativuse7
- Material
- chilling‐sensitive cultivare4
- Treatment
- chilling stresse4
- Gene
- NDHe4
- Outcome
- the activity of the chloroplast NADH dehydrogenase‐like complex (NDH)e4
- Direction
- loste4
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“we compared two cucumber cultivars with different susceptibilities to PSI photoinhibition and chilling stress tolerance”
e4“Indeed, the chilling‐sensitive cultivar lost the activity of the chloroplast NADH dehydrogenase‐like complex (NDH) due to destabilization of the PSI–NDH supercomplex under chilling stress, resulting in severe Fd over‐reduction”
Claim · model-written summary
Destabilization of the PSI–NDH supercomplex under chilling stress is suggested to explain the loss of NDH activity in the sensitive cultivar.
- Taxon
- cucumberCucumis sativuse7
- Outcome
- the activity of the chloroplast NADH dehydrogenase‐like complex (NDH)e4
- Qualifier
- destabilization of the PSI–NDH supercomplex under chilling stresse4
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“we compared two cucumber cultivars with different susceptibilities to PSI photoinhibition and chilling stress tolerance”
e4“Indeed, the chilling‐sensitive cultivar lost the activity of the chloroplast NADH dehydrogenase‐like complex (NDH) due to destabilization of the PSI–NDH supercomplex under chilling stress, resulting in severe Fd over‐reduction”
Claim · model-written summary
The chilling-tolerant cultivar maintained NDH activity under chilling stress.
- Taxon
- cucumberCucumis sativuse7
- Material
- chilling‐tolerant cultivare5
- Treatment
- chilling stresse5
- Gene
- NDHe5
- Outcome
- NDH activitye5
- Direction
- maintainede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“we compared two cucumber cultivars with different susceptibilities to PSI photoinhibition and chilling stress tolerance”
e5“By contrast, the chilling‐tolerant cultivar maintained NDH activity and suppressed reactive oxygen species and PSI photoinhibition during chilling stress”
Claim · model-written summary
The chilling-tolerant cultivar suppressed reactive oxygen species and PSI photoinhibition under chilling stress.
- Taxon
- cucumberCucumis sativuse7
- Material
- chilling‐tolerant cultivare5
- Treatment
- chilling stresse5
- Outcome
- reactive oxygen species and PSI photoinhibitione5
- Direction
- suppressede5
Not reportedComparator
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“we compared two cucumber cultivars with different susceptibilities to PSI photoinhibition and chilling stress tolerance”
e5“By contrast, the chilling‐tolerant cultivar maintained NDH activity and suppressed reactive oxygen species and PSI photoinhibition during chilling stress”
Claim · model-written summary
NDH functions as a crucial electron sink that prevents PSI photoinhibition.
- Taxon
- cucumberCucumis sativuse7
- Gene
- NDHe6
- Outcome
- PSI photoinhibitione6
- Qualifier
- crucial electron sinke6
Organisms pictured · Representative pictures of the matched species, not figures from the paper.
Verbatim
e7“we compared two cucumber cultivars with different susceptibilities to PSI photoinhibition and chilling stress tolerance”
e6“This study provides evidence that NDH functions as a crucial electron sink to prevent PSI photoinhibition”
The same questions in other papers
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- no effect
- negative
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- negative
- negative
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See all 13 papers →Claims are grouped by organism, treatment and what was measured or targeted. A paper counts as positive when any of its claims in the group is positive.